LOCUS       sequence-183086-        9039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Y2H Gal4 DNA binding domain for Prp28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pGBKT7-PRP28
FEATURES             Location/Qualifiers
     source          1..9039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3074..3121
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3148..3335
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3203..3227)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3359..3375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3359..3375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3372..3394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3383..3399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3407..3413,3414..3431,3432..3437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3452..3473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3590..3607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3761..4349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3841..3860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4678..4725
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4750..4769
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4957..5751)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4957..5751)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5068..5087)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5678..5697
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5752..5856)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5883..7225
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6846..6893)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7266..7284
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7322..7344)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7444..7463
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7484..7765
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7766..8440
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8543..8998)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8680..8701)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8892..8911
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcaa gaccaatcga tgttagtcag ctgattgctg
     1321 gtataaacaa gaaaaaagga ctagatgaaa atacatcagg taaaatcagc aagcctaggt
     1381 ttttgaataa acaagaaaga tctaagcaag aacgtttgaa ggagaacgaa gagagtttga
     1441 cgcctacgca aagtgatagc gctaaggtgg agattaaaaa ggtaaactct agagatgaca
     1501 gttttttcaa tgaaacaaat gataagaaga gaaatccatc aaagcaaaat ggctctaaat
     1561 ttcacttttc ttggaatgaa agcgaagata ctctgagcgg atatgatcct atcgtttcaa
     1621 ctagagctat agatttgcta tggaagggaa agactcctaa aaatgctgca gaatcttcat
     1681 atatggggaa gcattggact gagaagtcct tgcacgaaat gaatgagaga gattggagga
     1741 tactaaaaga agactacgct attgttacaa aaggtgggac agtagaaaat ccattgagaa
     1801 attgggaaga gctcaatata ataccaaggg acttgttacg tgttattata caggagctgc
     1861 gatttccttc accaactcct attcaaagga ttactattcc aaacgtttgc aatatgaaac
     1921 aatatagaga ttttcttggt gttgcctcta caggttcggg taagactttg gcctttgtaa
     1981 taccgatact aatcaagatg agtaggtcac ctccaaggcc accatctttg aagattattg
     2041 atggccccaa agcgttaatt ttagctccaa caagggaatt agtgcagcaa attcaaaaag
     2101 aaacacaaaa agttacaaaa atatggtcca aagagagcaa ctatgactgc aaagtaattt
     2161 ctattgttgg tggacactcc ttggaggaaa tttcattttc tctatcggag ggttgtgaca
     2221 tcctggtggc cactccagga cgtttaattg actctttaga aaatcactta cttgtgatga
     2281 aacaggtgga gacattagta ctagatgaag ctgacaaaat gattgattta ggttttgagg
     2341 accaagtaac aaacatttta actaaagtcg atataaatgc tgactctgct gtgaatagac
     2401 aaaccctgat gtttaccgct acaatgacac ccgttataga aaaaattgca gcaggataca
     2461 tgcaaaagcc tgtttatgca accattgggg ttgagacggg ttctgaacct ttgattcaac
     2521 aggttgtgga atatgcagat aatgacgaag acaaattcaa aaagttgaag ccaattgtcg
     2581 ctaaatatga tccaccaata ataattttta tcaattacaa acaaactgct gattggttag
     2641 cagaaaagtt tcaaaaggaa accaacatga aggtgacgat tttacatggt tctaaatcgc
     2701 aagagcaaag ggaacattct ttgcaattat ttcgcaccaa caaagtccaa atcatgatcg
     2761 caacaaacgt tgccgcaaga ggattggata tccctaatgt ttccttggta gtcaatttcc
     2821 agatttccaa aaaaatggat gactacatcc atagaattgg tagaacaggt cgtgcagcaa
     2881 atgagggcac tgctgtctcc tttgttagtg ccgctgaaga tgaatcattg atacgtgaac
     2941 tttacaaata cgtcaggaaa catgatccct tgaatagcaa tatcttttcc gaagccgtaa
     3001 agaacaaata taacgtcggc aaacagctca gtaatgagat tatatattaa gtcgacctgc
     3061 agcggccgca taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     3121 gcgcgcttgc agccaagcta attccgggcg aatttcttat gatttatgat ttttattatt
     3181 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     3241 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
     3301 catgaggtcg ctcttattga ccacacctct accggcatgc aagcttggcg taatcatggt
     3361 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3421 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     3481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     3541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3721 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3781 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3961 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4081 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4141 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4201 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4261 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4321 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4381 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4441 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4501 agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt
     4561 cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat
     4621 ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa
     4681 caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc
     4741 cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact
     4801 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     4861 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
     4921 tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     4981 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     5041 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     5101 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     5161 catgatattc ggcaagcagg catcgccatg agtcacgacg agatcctcgc cgtcgggcat
     5221 gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     5281 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     5341 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     5401 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     5461 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     5521 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt
     5581 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     5641 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     5701 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tactcttcct
     5761 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5821 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5881 tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt
     5941 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat
     6001 tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct
     6061 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc
     6121 gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga
     6181 gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa
     6241 tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt
     6301 ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac
     6361 tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg
     6421 atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc
     6481 agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta
     6541 cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta
     6601 aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa
     6661 ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga
     6721 gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag
     6781 tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc
     6841 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt
     6901 ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc
     6961 acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag
     7021 tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt
     7081 ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac
     7141 taccctttag ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg
     7201 ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg acattaacct
     7261 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     7321 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7381 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     7441 atgcggcatc agagcagatt gtactgagag tgcaccatag atcaacgaca ttactatata
     7501 tataatatag gaagcattta atagaacagc atcgtaatat atgtgtactt tgcagttatg
     7561 acgccagatg gcagtagtgg aagatattct ttattgaaaa atagcttgtc accttacgta
     7621 caatcttgat ccggagcttt tctttttttg ccgattaaga attaattcgg tcgaaaaaag
     7681 aaaaggagag ggccaagagg gagggcattg gtgactattg agcacgtgag tatacgtgat
     7741 taagcacaca aaggcagctt ggagtatgtc tgttattaat ttcacaggta gttctggtcc
     7801 attggtgaaa gtttgcggct tgcagagcac agaggccgca gaatgtgctc tagattccga
     7861 tgctgacttg ctgggtatta tatgtgtgcc caatagaaag agaacaattg acccggttat
     7921 tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat agttcaggca ctccgaaata
     7981 cttggttggc gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg tcaatgatta
     8041 cggcattgat atcgtccaac tgcatggaga tgagtcgtgg caagaatacc aagagttcct
     8101 cggtttgcca gttattaaaa gactcgtatt tccaaaagac tgcaacatac tactcagtgc
     8161 agcttcacag aaacctcatt cgtttattcc cttgtttgat tcagaagcag gtgggacagg
     8221 tgaacttttg gattggaact cgatttctga ctgggttgga aggcaagaga gccccgaaag
     8281 cttacatttt atgttagctg gtggactgac gccagaaaat gttggtgatg cgcttagatt
     8341 aaatggcgtt attggtgttg atgtaagcgg aggtgtggag acaaatggtg taaaagactc
     8401 taacaaaata gcaaatttcg tcaaaaatgc taagaaatag gttattactg agtagtattt
     8461 atttaagtat tgtttgtgca cttgccgatc tatgcggtgt gaaataccgc acagatgcgt
     8521 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     8581 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     8641 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     8701 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     8761 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     8821 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     8881 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     8941 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     9001 attcgccatt caggctgcgc aactgttggg aagggcgat
//
