LOCUS       sequence-183087-        9582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Y2H Gal4 DNA binding domain for Prp43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pGBKT7-PRP43
FEATURES             Location/Qualifiers
     source          1..9582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3617..3664
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3691..3878
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3746..3770)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3902..3918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3902..3918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3915..3937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3926..3942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3950..3956,3957..3974,3975..3980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3995..4016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4133..4150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4304..4892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4384..4403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5221..5268
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5293..5312
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5500..6294)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5500..6294)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5611..5630)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6221..6240
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6295..6399)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6426..7768
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7389..7436)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7809..7827
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7865..7887)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7987..8006
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8027..8308
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8309..8983
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(9086..9541)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9223..9244)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9435..9454
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatgggtt ccaaaagaag attctcgtcc gaacacccgg
     1321 atccagttga gacctctatt cctgagcagg ccgccgagat tgcggaagaa ttatccaaac
     1381 agcatcctct tccatccgaa gagccacttg tccatcatga tgctggtgaa ttcaagggct
     1441 tgcaacgtca ccatacttca gcagaagagg cccaaaaact tgaagatgga aaaataaatc
     1501 catttaccgg tagggagttt acaccaaaat atgttgatat cctgaaaatt agaagagaat
     1561 tgccagtaca tgcccagaga gatgagtttt taaaacttta tcaaaataac cagattatgg
     1621 tttttgttgg tgaaacaggt tccggtaaaa cgacacaaat tcctcaattc gttttattcg
     1681 atgagatgcc acacttagag aacactcaag ttgcatgtac tcaacctcgt cgtgtcgcag
     1741 caatgtctgt tgcacagaga gttgctgagg aaatggatgt caagttgggc gaagaagttg
     1801 gttattctat cagattcgaa aataaaactt ctaataaaac gatattgaag tatatgactg
     1861 atggtatgtt gttgagagag gcaatggaag accatgactt gtcgcgttat tcatgtatca
     1921 ttctggatga agcgcacgaa cgtacactag ctactgatat tttgatgggt cttttgaagc
     1981 aagtagtcaa gaggagacct gatttgaaga taattattat gtctgctact ctggatgcag
     2041 agaagttcca acgctacttc aacgatgctc cattacttgc cgttccgggc agaacatatc
     2101 cagttgagct atactataca ccggaatttc aaagggacta tttagattct gccattcgta
     2161 ctgttttgca aattcatgcc actgaagagg ctggcgatat tttgttattt ttaactggtg
     2221 aagatgaaat tgaagacgct gtcagaaaga tttccttaga aggtgatcaa ttggttagag
     2281 aagagggatg cggaccttta tccgtatacc cactgtatgg ttcattacca ccgcatcaac
     2341 aacaaagaat attcgagcct gctccagagt cacataatgg tagaccaggc aggaaagttg
     2401 ttatctccac gaacatcgct gaaacttctt taacaattga tggtattgtc tacgtcgttg
     2461 acccaggatt ttcaaagcaa aaagtttaca atccaagaat tagagttgag tctttacttg
     2521 tttcccctat ctccaaggct tctgcccaac aaagagctgg tcgtgctggt cgtacaaggc
     2581 ctggtaaatg tttcagatta tacactgaag aggcattcca aaaggaactg atagagcaaa
     2641 gttacccaga aattttacgt tccaatttat cctccaccgt tctagaacta aaaaaattag
     2701 gtattgatga cttggtccat ttcgatttta tggatccacc ggctccggaa acaatgatga
     2761 gagcccttga agaactaaat tatttggcat gtctggacga cgaaggtaac ttaacacccc
     2821 taggtagatt ggcatcccag tttcccttgg atcctatgct agcggtgatg ttaattgggt
     2881 cctttgagtt ccaatgttct caagaaatac taactatagt ggccatgcta tcggttccaa
     2941 acgtgtttat tcgcccaaca aaggacaaga agcgagcaga tgatgccaag aatatctttg
     3001 ctcatccaga cggtgatcat atcaccttac taaatgtata tcatgcgttc aaatcggatg
     3061 aagcttatga atatggtatc cataagtggt gccgtgacca ctatctaaat tacaggtccc
     3121 tttccgctgc tgataatatc cgctcccaat tagaaagatt aatgaaccgt tacaacctag
     3181 aattaaacac tacagactat gaaagcccta aatactttga caacatcaga aaggctcttg
     3241 cgtctgggtt tttcatgcaa gtagcaaaaa aaagatcggg agctaaagga tacatcacag
     3301 ttaaagataa ccaagatgtt ctgatccatc ctagtacggt tcttggccac gacgcagaat
     3361 gggtcattta caatgaattt gtgctcacat cgaagaacta cataagaact gtgacctcgg
     3421 tcaggcccga atggctgatt gaaatagcgc ctgcatacta cgacttaagc aatttccaaa
     3481 aaggtgatgt caaattatct ttggaaagga tcaaggaaaa agttgataga ttaaacgagt
     3541 tgaaacaagg taaaaacaaa aagaagagta agcactccaa gaaataggga tccgtcgacc
     3601 tgcagcggcc gcataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     3661 tttgcgcgct tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt
     3721 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     3781 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     3841 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg gcgtaatcat
     3901 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3961 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4021 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4081 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4141 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4201 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4261 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4321 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4381 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4441 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4501 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4561 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4621 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4681 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4741 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4801 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4861 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4921 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4981 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5041 atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc
     5101 cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc
     5161 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
     5221 gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg
     5281 ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga
     5341 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     5401 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     5461 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     5521 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     5581 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     5641 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     5701 caccatgata ttcggcaagc aggcatcgcc atgagtcacg acgagatcct cgccgtcggg
     5761 catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5821 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5881 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5941 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     6001 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     6061 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc
     6121 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     6181 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     6241 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatactctt
     6301 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6421 acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa
     6481 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc
     6541 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc
     6601 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag
     6661 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg
     6721 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta
     6781 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac
     6841 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat
     6901 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata
     6961 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg
     7021 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt
     7081 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt
     7141 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt
     7201 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa
     7261 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta
     7321 cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa
     7381 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag
     7441 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     7501 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     7561 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     7621 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     7681 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     7741 atgctatcat ttcctttgat attggatcat actaagaaac cattattatc atgacattaa
     7801 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     7861 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7921 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     7981 actatgcggc atcagagcag attgtactga gagtgcacca tagatcaacg acattactat
     8041 atatataata taggaagcat ttaatagaac agcatcgtaa tatatgtgta ctttgcagtt
     8101 atgacgccag atggcagtag tggaagatat tctttattga aaaatagctt gtcaccttac
     8161 gtacaatctt gatccggagc ttttcttttt ttgccgatta agaattaatt cggtcgaaaa
     8221 aagaaaagga gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt
     8281 gattaagcac acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg
     8341 tccattggtg aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc
     8401 cgatgctgac ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt
     8461 tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa
     8521 atacttggtt ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga
     8581 ttacggcatt gatatcgtcc aactgcatgg agatgagtcg tggcaagaat accaagagtt
     8641 cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca tactactcag
     8701 tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag caggtgggac
     8761 aggtgaactt ttggattgga actcgatttc tgactgggtt ggaaggcaag agagccccga
     8821 aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag
     8881 attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga
     8941 ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta
     9001 tttatttaag tattgtttgt gcacttgccg atctatgcgg tgtgaaatac cgcacagatg
     9061 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     9121 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     9181 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     9241 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     9301 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     9361 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     9421 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     9481 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     9541 tccattcgcc attcaggctg cgcaactgtt gggaagggcg at
//
