LOCUS       sequence-183090-        7768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is the P1-M-P2 portion of the Bcl-2 promoter with a luciferase 
            reporter in pGL3Basic plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Bredow S, Juri DE, Cardon K, Tesfaigzi Y
  TITLE     Identification of a novel Bcl-2 promoter region that counteracts in 
            a p53-dependent manner the inhibitory P2 region.
  JOURNAL   Gene. 2007 Dec 1;404(1-2):110-6. Epub 2007 Sep 14.
  PUBMED    17913397
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2007-12-1"; volume: "404(1-2)"; pages: "110-6. Epub 2007 Sep 
            14"
COMMENT     Sequence Label: P1-M-P2
FEATURES             Location/Qualifiers
     source          1..7768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    2127..2218
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(2159..2178)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    2232..2280
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2411..2866)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2548..2569)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2760..2779
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2893..2997
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2998..3066,3067..3858)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3216..3235)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4029..4617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4518..4537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4771..4788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5036..5157
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5073..5092)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5127..5146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(5198..6850)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     6766..6784
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      6847..6856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggggccttct gctcaggcgt gcggcaggcc gcgtgcggac ttggtggtcg ctggggtccg
       61 cgacggggtg ggggctcccg gggaaccgca cgcggccggg ccgggcggac gacggagtgc
      121 ggaggggggc ggctggcggg agggtgcgcc atgaaaacaa gggctggaaa agcgccggga
      181 accgcctgga ccctttctgg ccgtgtgagt gtgtgtggag tgtgtgtgtg tgtgtgtgtg
      241 tgtgtgtctc gcctggacct tttctagccg tgtatgtggg agtgtgtgtg tcgcctggac
      301 cctttctagc cgtgtatgag agtgtgtaca cgcgcctaca cacacacacg ttgtgttacc
      361 ggcgctcggc cgccggggga agacccaggc caatgccgcc ccccaccgcc cccagcagtg
      421 agacctcagc gctgccctgc tgtgaagaca ggtgactctg cacgttttaa gcaatgtcta
      481 gggacgcccc gagcgtggtg tttactttca agtagcttcc taggtgtccg cgcactacac
      541 acgcacgcgc atccccgccc gtgtccacct gaacacctag tccgtggccc aggccatgca
      601 gaactcagcg ctccagggaa ggggtttatc aagggcttta cgacagttta agtcaatgtt
      661 ttccctctgt ccctaacacc ttttacactg gtttagtgct acacgatgag gacttccata
      721 tagtaacttt caggcccacc gtcctaacgc tggggtgggt gggctcctaa aggtctccac
      781 ctttgcctcg tagccaatcc tagttggccg cactttctca aatgaggtac atagatacac
      841 acatacacat tcacactcgc agacgtgtgt acgcgcaagc agacagtttt aaattaatca
      901 gcatgaatag cccctagcaa aaaaggacaa gaggacaaac aagttgcacg tgtgtatttt
      961 tatctccaag agtctttgac aaaaagcacc aagaaaagaa aaaggtcttc tagaagcaca
     1021 gcggcttact taatagggct cgagtgcaaa tatataggga cactggatta ttggggtcaa
     1081 gaaagatgac aaatgagtac tacaatgtgt acaaatatgc tatgactttt tttttttttt
     1141 tgagacagag tcttgctctg ttgtatcttg gaggctggtg tgcagtggtg cgatctcagc
     1201 tcactgcaac ctctgcctcc caggctcaag caattctgcc tcagcctcct gagtagctgg
     1261 gattacacgt gcacgccact acgctctgct aatttttgta tttttagtag agacggggtt
     1321 tcgccatact ggccaggctg gtctcgaact cctgacctcg ttatccgcct gcctcagcct
     1381 cccaaagtgc tgggattaca gacatgagcc actgtgccct gcctgacatc tttattttgt
     1441 ctcattccta ctctaaaatc aaaacattct ttaaaaagcc agaaaagcac accaaattat
     1501 gattttttta atttttactt agttggcctt tttttttttt ttttaagaga tggcttttgc
     1561 tatgttgccc aggctggact cgaacttctg ggctcgaagg attctcccac ctcagcctca
     1621 tgaatagctg ggactatacg cactgccatc tctcttgggt gtttttattt tatttcttca
     1681 tttattgtat tccatcagcc caacaagcca acttctctca cataggaatg ggggcctggg
     1741 tacaaatttc ttatccaggc aagaggggat gaaaggagat cctccttctt gcccttctga
     1801 tggttgggac agagtccctt ttatgcgcgt gggaggtgtt ttggtctgga tagacactgg
     1861 gtatagattg acctagaata aatgttctca cataaattca taccaattgc acaacagtat
     1921 ggtttagaaa tcttgaaagc gtattttaaa gccccaaaat tacctaagag gaattgaaag
     1981 gcttcaagct cctaatactc agcatatgag tgaggaaatg ataggacaaa cctgcaaaag
     2041 aaactgtact tctgcaaagg gggcagccag aaggaaacgg atctcgagcc cgggctagca
     2101 cgcgtaagag ctcggtacct atcgatagag aaatgttctg gcacctgcac ttgcactggg
     2161 gacagcctat tttgctagtt tgttttgttt cgttttgttt tgatggagag cgtatgttag
     2221 tactatcgat tcacacaaaa aaccaacaca cagatgtaat gaaaataaag atattttatt
     2281 gcggccgctc caagtacctc ccgtacctta atattactta cttatcatgg tagcttgggc
     2341 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2401 ggcgaatggc aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     2461 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     2521 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     2581 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     2641 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     2701 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     2761 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     2821 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     2881 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2941 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3001 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3061 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3121 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3181 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3241 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3301 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3361 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3421 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3481 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3541 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3601 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3661 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3721 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3781 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3841 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3901 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3961 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4021 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4081 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4141 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4201 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4261 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4321 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4381 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4441 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4501 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4561 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4621 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4681 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4741 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4801 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
     4861 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     4921 cggtcgacgg atccttatcg attttaccac atttgtagag gttttacttg ctttaaaaaa
     4981 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5041 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5101 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5161 tgtctgctcg aagcggccgg ccgccccgac tctagaatta cacggcgatc tttccgccct
     5221 tcttggcctt tatgaggatc tctctgattt ttcttgcgtc gagttttccg gtaagacctt
     5281 tcggtacttc gtccacaaac acaactcctc cgcgcaactt tttcgcggtt gttacttgac
     5341 tggcgacgta atccacgatc tctttttccg tcatcgtctt tccgtgctcc aaaacaacaa
     5401 cggcggcggg aagttcaccg gcgtcatcgt cgggaagacc tgcgacacct gcgtcgaaga
     5461 tgttggggtg ttggagcaag atggattcca attcagcggg agccacctga tagcctttgt
     5521 acttaatcag agacttcagg cggtcaacga tgaagaagtg ttcgtcttcg tcccagtaag
     5581 ctatgtctcc agaatgtagc catccatcct tgtcaatcaa ggcgttggtc gcttccggat
     5641 tgtttacata accggacata atcataggac ctctcacaca cagttcgcct ctttgattaa
     5701 cgcccagcgt tttcccggta tccagatcca caaccttcgc ttcaaaaaat ggaacaactt
     5761 taccgaccgc gcccggttta tcatccccct cgggtgtaat cagaatagct gatgtagtct
     5821 cagtgagccc atatccttgc ctgatacctg gcagatggaa cctcttggca accgcttccc
     5881 cgacttcctt agagagggga gcgccaccag aagcaatttc gtgtaaatta gataaatcgt
     5941 atttgtcaat cagagtgctt ttggcgaaga aggagaatag ggttggcacc agcagcgcac
     6001 tttgaatctt gtaatcctga aggctcctca gaaacagctc ttcttcaaat ctatacatta
     6061 agacgactcg aaatccacat atcaaatatc cgagtgtagt aaacattcca aaaccgtgat
     6121 ggaatggaac aacacttaaa atcgcagtat ccggaatgat ttgattgcca aaaataggat
     6181 ctctggcatg cgagaatctc acgcaggcag ttctatgagg cagagcgaca cctttaggca
     6241 gaccagtaga tccagaggag ttcatgatca gtgcaattgt cttgtcccta tcgaaggact
     6301 ctggcacaaa atcgtattca ttaaaaccgg gaggtagatg agatgtgacg aacgtgtaca
     6361 tcgactgaaa tccctggtaa tccgttttag aatccatgat aataattttt tggatgattg
     6421 ggagcttttt ttgcacgttc aaaatttttt gcaacccctt tttggaaacg aacaccacgg
     6481 taggctgcga aatgcccata ctgttgagca attcacgttc attataaatg tcgttcgcgg
     6541 gcgcaactgc aactccgata aataacgcgc ccaacaccgg cataaagaat tgaagagagt
     6601 tttcactgca tacgacgatt ctgtgatttg tattcagccc atatcgtttc atagcttctg
     6661 ccaaccgaac ggacatttcg aagtactcag cgtaagtgat gtccacctcg atatgtgcat
     6721 ctgtaaaagc aattgttcca ggaaccaggg cgtatctctt catagcctta tgcagttgct
     6781 ctccagcggt tccatcttcc agcggataga atggcgccgg gcctttcttt atgtttttgg
     6841 cgtcttccat ggtggcttta ccaacagtac cggaatgcca agctttcctg tgcagagaac
     6901 ttacttgtat tttttaagta cagcatgatc ctctgtcaag tttccttttt gtaaaaccaa
     6961 aacaaatgca taaggcaacg atcccatcaa tcttcagcac tctccagtta tagctgattt
     7021 gaaacttccc aatgaatcag gagtcggggg gagagggagt aaaaattagg agggtttcca
     7081 gatcgattcc cagacttctg cttcacagaa atgtcaatcc gcaggaatcc caaccggaga
     7141 tctcaagagc tcgagaaaaa aaaaaggcag cggcggcggc agatgaatta caattttcag
     7201 tccggtattc gcagaagtcc tgtgatgttt tccccttctc ggcaatttac acgcgcgcac
     7261 acacgcgcgg gcacaggcat gaatctctat ccacgggacc gcttcacgcc tccccaggag
     7321 agagacaggg gagatgggac gatgaaggag ccggggacgg aggcaggaat cctcttctga
     7381 ttaaactccg aacagcaaat gcattttccg aaaagctgct ggataaatga aggcaggacg
     7441 cgcctggccc gccggtgccg agcgctagaa gcccgcgctg tgtgtggtgc ggcgaggggt
     7501 ggggagaagg aggtggtggg ggagggtttt attttttccc tcttttccta aaaaggatga
     7561 ctgctacgaa gttctccccc ctggaccccc tcttccgctg caccccaccg gcgcaccccg
     7621 cctccgggct gcgcaccctt tctcctcctc ctggtcctgc gcggcggcgc tggctacggc
     7681 cgcctcccgg agctcccgcc gcgcagcccg ctccgagcgc tgacggccgc cggcagggag
     7741 ggcccggagc cccggcacct tcgctggc
//
