LOCUS       sequence-183091-        7089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is the P1 part of the Bcl-2 promoter with a luciferase reporter
            in pGL3Basic plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Bredow S, Juri DE, Cardon K, Tesfaigzi Y
  TITLE     Identification of a novel Bcl-2 promoter region that counteracts in 
            a p53-dependent manner the inhibitory P2 region.
  JOURNAL   Gene. 2007 Dec 1;404(1-2):110-6. Epub 2007 Sep 14.
  PUBMED    17913397
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2007-12-1"; volume: "404(1-2)"; pages: "110-6. Epub 2007 Sep 
            14"
COMMENT     Sequence Label: P1
FEATURES             Location/Qualifiers
     source          1..7089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    2122..2213
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(2154..2173)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    2227..2275
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2406..2861)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2543..2564)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2755..2774
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2888..2992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2993..3061,3062..3853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3211..3230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4024..4612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4513..4532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4766..4783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5031..5152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5068..5087)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5122..5141
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(5193..6845)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     6761..6779
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      6842..6851
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             7022..7037
                     /estimated_length=16
ORIGIN
        1 cgggccggcg gcagggccgc gtgcggactt ggtggtcgct ggggtccgcg acggggtggg
       61 ggctcccggg gaaccgcacg cggccgggcc gggcggacga cggagtgcgg aggggggcgg
      121 ctggcgggag ggtgcgccat gaaaacaagg gctggaaaag cgccgggaac cgcctggacc
      181 ctttctggcc gtgtgagtgt gtgtggagtg tgtgtgtgtg tgtgtgtgtg tgtgtctcgc
      241 ctggaccttt tctagccgtg tatgtgggag tgtgtgtgtc gcctggaccc tttctagccg
      301 tgtatgagag tgtgtacacg cgcctacaca cacacacgtt gtgttaccgg cgctcggccg
      361 ccgggggaag acccaggcca atgccgcccc ccaccgcccc cagcagtgag acctcagcgc
      421 tgccctgctg tgaagacagg tgactctgca cgttttaagc aatgtctagg gacgccccga
      481 gcgtggtgtt tactttcaag tagcttccta ggtgtccgcg cactacacac gcacgcgcat
      541 ccccgcccgt gtccacctga acacctagtc cgtggcccag gccatgcaga actcagcgct
      601 ccagggaagg ggtttatcaa gggctttacg acagtttaag tcaatgtttt ccctctgtcc
      661 ctaacacctt ttacactggt ttagtgctac acgatgagga cttccatata gtaactttca
      721 ggcccaccgt cctaacgctg gggtgggtgg gctcctaaag gtctccacct ttgcctcgta
      781 gccaatccta gttggccgca ctttctcaaa tgaggtacat agatacacac atacacattc
      841 acactcgcag acgtgtgtac gcgcaagcag acagttttaa attaatcagc atgaatagcc
      901 cctagcaaaa aaggacaaga ggacaaacaa gttgcacgtg tgtattttta tctccaagag
      961 tctttgacaa aaagcaccaa gaaaagaaaa aggtcttcta gaagcacagc ggcttactta
     1021 atagggctcg agtgcaaata tatagggaca ctggattatt ggggtcaaga aagatgacaa
     1081 atgagtacta caatgtgtac aaatatgcta tgactttttt tttttttttg agacagagtc
     1141 ttgctctgtt gtatcttgga ggctggtgtg cagtggtgcg atctcagctc actgcaacct
     1201 ctgcctccca ggctcaagca attctgcctc agcctcctga gtagctggga ttacacgtgc
     1261 acgccactac gctctgctaa tttttgtatt tttagtagag acggggtttc gccatactgg
     1321 ccaggctggt ctcgaactcc tgacctcgtt atccgcctgc ctcagcctcc caaagtgctg
     1381 ggattacaga catgagccac tgtgccctgc ctgacatctt tattttgtct cattcctact
     1441 ctaaaatcaa aacattcttt aaaaagccag aaaagcacac caaattatga tttttttaat
     1501 ttttacttag ttggcctttt tttttttttt ttaagagatg gcttttgcta tgttgcccag
     1561 gctggactcg aacttctggg ctcgaaggat tctcccacct cagcctcatg aatagctggg
     1621 actatacgca ctgccatctc tcttgggtgt ttttatttta tttcttcatt tattgtattc
     1681 catcagccca acaagccaac ttctctcaca taggaatggg ggcctgggta caaatttctt
     1741 atccaggcaa gaggggatga aaggagatcc tccttcttgc ccttctgatg gttgggacag
     1801 agtccctttt atgcgcgtgg gaggtgtttt ggtctggata gacactgggt atagattgac
     1861 ctagaataaa tgttctcaca taaattcata ccaattgcac aacagtatgg tttagaaatc
     1921 ttgaaagcgt attttaaagc cccaaaatta cctaagagga attgaaaggc ttcaagctcc
     1981 taatactcag catatgagtg aggaaatgat aggacaaacc tgcaaaagaa actgtacttc
     2041 tgcaaagggg gcagccagaa ggaaacggat ctgcagcccg ggggatccac tagcacgcgt
     2101 aagagctcgg tacctatcga tagagaaatg ttctggcacc tgcacttgca ctggggacag
     2161 cctattttgc tagtttgttt tgtttcgttt tgttttgatg gagagcgtat gttagtacta
     2221 tcgattcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattgcggc
     2281 cgctccaagt acctcccgta ccttaatatt acttacttat catggtagct tgggctggcg
     2341 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2401 atggcaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2461 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2521 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2581 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2641 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2701 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2761 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2821 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     2881 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2941 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3001 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3061 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3181 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3301 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3541 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3601 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3721 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3781 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3841 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4681 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4741 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgctg
     4801 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     4861 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     4921 gacggatcct tatcgatttt accacatttg tagaggtttt acttgcttta aaaaacctcc
     4981 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5161 gctcgaagcg gccggccgcc ccgactctag aattacacgg cgatctttcc gcccttcttg
     5221 gcctttatga ggatctctct gatttttctt gcgtcgagtt ttccggtaag acctttcggt
     5281 acttcgtcca caaacacaac tcctccgcgc aactttttcg cggttgttac ttgactggcg
     5341 acgtaatcca cgatctcttt ttccgtcatc gtctttccgt gctccaaaac aacaacggcg
     5401 gcgggaagtt caccggcgtc atcgtcggga agacctgcga cacctgcgtc gaagatgttg
     5461 gggtgttgga gcaagatgga ttccaattca gcgggagcca cctgatagcc tttgtactta
     5521 atcagagact tcaggcggtc aacgatgaag aagtgttcgt cttcgtccca gtaagctatg
     5581 tctccagaat gtagccatcc atccttgtca atcaaggcgt tggtcgcttc cggattgttt
     5641 acataaccgg acataatcat aggacctctc acacacagtt cgcctctttg attaacgccc
     5701 agcgttttcc cggtatccag atccacaacc ttcgcttcaa aaaatggaac aactttaccg
     5761 accgcgcccg gtttatcatc cccctcgggt gtaatcagaa tagctgatgt agtctcagtg
     5821 agcccatatc cttgcctgat acctggcaga tggaacctct tggcaaccgc ttccccgact
     5881 tccttagaga ggggagcgcc accagaagca atttcgtgta aattagataa atcgtatttg
     5941 tcaatcagag tgcttttggc gaagaaggag aatagggttg gcaccagcag cgcactttga
     6001 atcttgtaat cctgaaggct cctcagaaac agctcttctt caaatctata cattaagacg
     6061 actcgaaatc cacatatcaa atatccgagt gtagtaaaca ttccaaaacc gtgatggaat
     6121 ggaacaacac ttaaaatcgc agtatccgga atgatttgat tgccaaaaat aggatctctg
     6181 gcatgcgaga atctcacgca ggcagttcta tgaggcagag cgacaccttt aggcagacca
     6241 gtagatccag aggagttcat gatcagtgca attgtcttgt ccctatcgaa ggactctggc
     6301 acaaaatcgt attcattaaa accgggaggt agatgagatg tgacgaacgt gtacatcgac
     6361 tgaaatccct ggtaatccgt tttagaatcc atgataataa ttttttggat gattgggagc
     6421 tttttttgca cgttcaaaat tttttgcaac ccctttttgg aaacgaacac cacggtaggc
     6481 tgcgaaatgc ccatactgtt gagcaattca cgttcattat aaatgtcgtt cgcgggcgca
     6541 actgcaactc cgataaataa cgcgcccaac accggcataa agaattgaag agagttttca
     6601 ctgcatacga cgattctgtg atttgtattc agcccatatc gtttcatagc ttctgccaac
     6661 cgaacggaca tttcgaagta ctcagcgtaa gtgatgtcca cctcgatatg tgcatctgta
     6721 aaagcaattg ttccaggaac cagggcgtat ctcttcatag ccttatgcag ttgctctcca
     6781 gcggttccat cttccagcgg atagaatggc gccgggcctt tctttatgtt tttggcgtct
     6841 tccatggtgg ctttaccaac agtaccggaa tgccaagctt gatatcgaat tcctgcagcc
     6901 ccgccgcgca gcccgctccg agcgctgacg gccgccggca gggagggccc ggagccccgg
     6961 caccttcgct ggcagcggcg gcggcggcag cgcggcgggg ccacggagag cggcgggcgg
     7021 gnnnnnnnnn nnnnnnnggg caggcggcgc ggaggggcgg gcgcgggagg aagggggcgg
     7081 gagcggggc
//
