LOCUS       sequence-183098-       11112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector with CaMKIIa promoter and upstream CMV promoter 
            expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor
            membrane localization of the C1C2 opsin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Bedbrook CN, Kato M, Ravindra Kumar S, Lakshmanan A, Nath RD, Sun F,
            Sternberg PW, Arnold FH, Gradinaru V
  TITLE     Genetically Encoded Spy Peptide Fusion System to Detect Plasma 
            Membrane-Localized Proteins In Vivo.
  JOURNAL   Chem Biol. 2015 Aug 20;22(8):1108-21. doi: 
            10.1016/j.chembiol.2015.06.020. Epub 2015 Jul 23.
  PUBMED    26211362
REFERENCE   2  (bases 1 to 11112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2015.06.020"; journalName: "Chem Biol"; date: 
            "2015-08-20- 20"; volume: "22"; issue: "8"; pages: "1108-21"
COMMENT     Sequence Label: pLenti-CaMKIIa-SpyTag-C1C2-mCherry
FEATURES             Location/Qualifiers
     source          1..11112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        95..474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        476..674
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     629..649
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             692..872
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    919..1044
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1537..1770
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1955..1999
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2297..2414
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2459..3747
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             3846..3884
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             4929..5636
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5071..5089)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5339..5358
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5661..6249
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5714..5734)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6132..6143)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6252..6268)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6253..6269)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6774..6954
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6980..6997)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6986..7210
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7256..7684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7343..7362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7553..7574
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7693..7713)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7698..8027
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7878..8013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8075..8122
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8141..8515
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8645..8766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8682..8701)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8736..8755
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8815..8831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8815..8831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8828..8850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8839..8855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8863..8869,8870..8887,8888..8893))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8908..8929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9046..9063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9217..9805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9297..9316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9976..10767,10768..10836))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10599..10618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10837..10941)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11016..11035)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
       61 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      121 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      241 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      301 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      361 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      421 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      481 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      541 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      601 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      661 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
      721 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      781 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      841 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
      901 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
      961 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     1021 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     1081 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     1141 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     1201 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1261 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     1321 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     1381 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1441 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1501 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1561 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1621 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1681 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1741 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1801 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1861 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1921 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1981 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     2041 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     2101 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     2161 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     2221 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     2281 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2341 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2401 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaaca
     2461 ttatggcctt aggtcacttc atctccatgg ggttcttctt ctgattttct agaaaatgag
     2521 atgggggtgc agagagcttc ctcagtgacc tgcccagggt cacatcagaa atgtcagagc
     2581 tagaacttga actcagatta ctaatcttaa attccatgcc ttgggggcat gcaagtacga
     2641 tatacagaag gagtgaactc attagggcag atgaccaatg agtttaggaa agaagagtcc
     2701 agggcagggt acatctacac cacccgccca gccctgggtg agtccagcca cgttcacctc
     2761 attatagttg cctctctcca gtcctacctt gacgggaagc acaagcagaa actgggacag
     2821 gagccccagg agaccaaatc ttcatggtcc ctctgggagg atgggtgggg agagctgtgg
     2881 cagaggcctc aggaggggcc ctgctgctca gtggtgacag ataggggtga gaaagcagac
     2941 agagtcattc cgtcagcatt ctgggtctgt ttggtacttc ttctcacgct aaggtggcgg
     3001 tgtgatatgc acaatggcta aaaagcaggg agagctggaa agaaacaagg acagagacag
     3061 aggccaagtc aaccagacca attcccagag gaagcaaaga aaccattaca gagactacaa
     3121 gggggaaggg aaggagagat gaattagctt cccctgtaaa ccttagaacc cagctgttgc
     3181 cagggcaacg gggcaatacc tgtctcttca gaggagatga agttgccaga gtaactacat
     3241 cctgtctttc tcaaggacca tcccagaatg tggcacccac tagccgttac catagcaact
     3301 gcctctttgc cccacttaat cccatcccgt ctgttaaaag ggccctatag ttggaggtgg
     3361 gggaggtagg aagagcgatg atcacttgtg gactaagttt gttcgcatcc ccttctccaa
     3421 ccccctcagt acatcaccct gggggaacag ggtccacttg ctcctgggcc cacacagtcc
     3481 tgcagtattg tgtatataag gccagggcaa agaggagcag gttttaaagt gaaaggcagg
     3541 caggtgttgg ggaggcagtt accggggcaa cgggaacagg gcgtttcgga ggtggttgcc
     3601 atggggacct ggatgctgac gaaggctcgc gaggctgtga gcagccacag tgccctgctc
     3661 agaagcccca agctcgtcag tcaagccggt tctccgtttg cactcaggag cacgggcagg
     3721 cgagtggccc ctagttctgg gggcagcggg ggatccccgg gtaccggtag gccaccatgt
     3781 cgcggaggcc atggcttctt gccctagcgc tggcagtggc gctggcggcc ggcagcgcag
     3841 gagccgccca catcgtgatg gtggacgcct acaagcccac caagtcgact ggcagtgacg
     3901 cgacggtgcc ggtcgcgact caggatggcc ccgactacgt tttccaccgt gcccacgagc
     3961 gcatgctctt ccaaacctca tacactcttg agaacaatgg ttctgttatt tgcatcccga
     4021 acaacggcca gtgcttctgc ttggcttggc ttaaatccaa cggaacaaat gccgagaagt
     4081 tggctgccaa cattctgcag tggattactt ttgcgctttc agcgctctgc ctgatgttct
     4141 acggctacca gacctggaag tctacttgcg gctgggagga gatttacgtg gccacgatcg
     4201 agatgatcaa gttcatcatc gagtatttcc atgagtttga cgaacctgcg gtgatctact
     4261 catccaacgg caacaagacc gtgtggcttc gttacgcgga gtggctgctg acctgccctg
     4321 tcattcttat ccatctgagc aaccttacgg gtctggcgaa cgactataac aagcgtacca
     4381 tgggtctgct ggtgtcagat atcggcacga tcgtgtgggg caccacggcc gcgctgtcca
     4441 agggatacgt ccgtgtcatt ttcttcctga tgggcctgtg ctacggcatc tacacattct
     4501 tcaacgcagc caaggtctac attgaggcgt accacaccgt gccgaagggc cggtgtcgcc
     4561 aggtggtgac tggcatggct tggctcttct tcgtatcatg gggtatgttc cccatcctgt
     4621 tcatcctcgg ccccgagggc ttcggcgtcc tgagcgtgta cggctccacc gtcggccaca
     4681 ccatcattga cctgatgtcg aagaactgct ggggtctgct cggccactac ctgcgcgtgc
     4741 tgatccacga gcatatcctc atccacggcg acattcgcaa gaccaccaaa ttgaacattg
     4801 gtggcactga gattgaggtc gagacgctgg tggaggacga ggccgaggct ggcgcggtcg
     4861 cggccgccaa gagcaggatc accagcgagg gcgagtacat ccccctggac cagatcgaca
     4921 tcaacgtggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     4981 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     5041 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     5101 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     5161 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     5221 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     5281 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     5341 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     5401 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     5461 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     5521 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     5581 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagaat
     5641 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5701 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5761 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5821 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5881 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5941 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6001 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6061 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6121 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6181 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6241 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     6301 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     6361 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6421 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6481 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6541 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6601 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     6661 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     6721 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6781 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6841 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6901 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6961 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7021 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7081 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7141 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     7201 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     7261 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7321 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7381 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7441 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7501 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7561 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7621 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7681 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7801 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7921 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7981 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     8041 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     8101 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     8161 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     8221 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     8281 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     8341 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     8401 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8461 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     8521 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8581 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8641 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8701 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8761 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8821 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8881 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8941 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9001 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9061 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9121 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9181 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9241 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9301 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9361 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9421 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9481 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9541 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9601 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9661 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9721 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9781 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9961 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10021 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10081 cttaccatct ggacccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10141 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10201 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10261 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10321 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10381 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10441 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10501 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10561 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10621 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10681 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10741 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10801 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10861 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10921 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10981 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    11041 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    11101 atttaagcta ca
//
