LOCUS       sequence-183107-        6339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of hnRNPC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6339)
  TITLE     Dreyfuss Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1+ hnRNPC myc
FEATURES             Location/Qualifiers
     source          1..6339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     87..106
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             241..1035
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(295..314)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     905..924
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1211..1332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1248..1267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1302..1321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1381..1397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1381..1397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1394..1416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1405..1421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1429..1435,1436..1453,1454..1459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1474..1495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1612..1629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1783..2371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1863..1882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2542..3333,3334..3402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3165..3184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3403..3507)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3582..3601)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3773..4152
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4153..4356
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4307..4327
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4401..4420
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4401..4419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5381..5410
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5426..5443
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5466..5483)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5472..5696
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5742..6170
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5829..5848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6039..6060
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6179..6199)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(6184..6339,1..174)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
       61 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
      121 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
      181 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
      241 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
      301 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
      361 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
      421 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
      481 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
      541 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
      601 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
      661 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
      721 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
      781 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
      841 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
      901 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
      961 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     1021 gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa
     1081 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     1141 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     1201 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1261 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1321 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     1381 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1441 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1501 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1561 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1621 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1681 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1741 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1801 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1861 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1921 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1981 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2041 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2101 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2161 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2221 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2281 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2341 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2401 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2461 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2521 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2581 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2641 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2701 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2761 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2821 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2881 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2941 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3001 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3061 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3121 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3181 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3241 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3301 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3361 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3421 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3481 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     3541 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     3601 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     3661 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     3721 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     3781 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     3841 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     3901 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     3961 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4021 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4081 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4141 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4201 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4261 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4321 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4381 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagtta
     4441 agcttacgat ggccagcaac gttaccaaca agacagatcc tcgctccatg aactcccgtg
     4501 tattcattgg gaatctcaac actcttgtgg tcaagaaatc tgatgtggag gcaatctttt
     4561 cgaagtatgg caaaattgtg ggctgctctg ttcataaggg ctttgccttc gttcagtatg
     4621 ttaatgagag aaatgcccgg gctgctgtag caggagagga tggcagaatg attgctggcc
     4681 aggttttaga tattaacctg gctgcagagc caaaagtgaa ccgaggaaaa gcaggtgtga
     4741 aacgatctgc agcggagatg tacggctcct cttttgactt ggactatgac tttcaacggg
     4801 actattatga taggatgtac agttacccag cacgtgtacc tcctcctcct cctattgctc
     4861 gggctgtagt gccctcgaaa cgtcagcgtg tatcaggaaa cacttcacga aggggcaaaa
     4921 gtggcttcaa ttctaagagt ggacagcggg gatcttccaa gtctggaaag ttgaaaggag
     4981 atgaccttca ggccattaag aaggagctga cccagataaa acaaaaagtg gattctctcc
     5041 tggaaaacct ggaaaaaatt gaaaaggaac agagcaaaca agcagtagag atgaagaatg
     5101 ataagtcaga agaggagcag agcagcagct ccgtgaagaa agatgagact aatgtgaaga
     5161 tggagtctga ggggggtgca gatgactctg ctgaggaggg ggacctactg gatgatgatg
     5221 ataatgaaga tcggggggat gaccagctgg agttgatcaa ggatgatgaa aaagaggctg
     5281 aggaaggaga ggatgacaga gacagcgcca atggcgagga tgactcgaat tctgcagata
     5341 tccagcacag tggcggccgc tcgagtctag agggcccttc gaacaaaaac tcatctcaga
     5401 agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt aaacccgctg
     5461 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5521 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5581 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5641 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5701 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5761 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5821 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5881 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5941 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6001 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6061 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6121 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     6181 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6241 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6301 agaagtatgc aaagcatgca tctcaattag tcagcaacc
//
