LOCUS       sequence-183112-        5979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5979)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Keratin-17
FEATURES             Location/Qualifiers
     source          1..5979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..702
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     596..619
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    827..948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(864..883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     918..937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(955..1410)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1092..1113)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1304..1323
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1437..1541
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1543..1900
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1543..1734
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1566..1586)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1751..1886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1935..2729
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1935..2729
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1989..2008)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2599..2618
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2917..2936)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2961..3008
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3337..3925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3826..3845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4100..4403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4404..4607
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4558..4578
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
      241 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
      361 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      421 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      481 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
      541 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
      601 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
      661 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaagcggc cgcgactcta
      721 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      781 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      841 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      901 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
      961 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     1021 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     1081 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     1141 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     1201 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     1261 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     1321 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     1381 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     1441 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1501 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     1561 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     1621 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     1681 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     1741 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     1801 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     1861 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     1921 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     1981 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     2041 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     2101 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     2161 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     2221 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     2281 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     2341 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     2401 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     2461 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     2521 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     2581 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     2641 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     2701 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     2761 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     2821 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     2881 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     2941 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     3001 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     3061 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     3121 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     3181 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3241 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3301 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3361 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3421 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3481 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3541 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3601 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3661 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3721 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3781 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3841 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3901 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3961 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4021 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
     4081 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4141 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4201 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4261 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4321 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4381 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4441 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4501 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4561 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     4621 gtcagatccg ctagcaccat gagcttcacc actcgctcca ccttctccac caactaccgg
     4681 tccctgggct ctgtccaggc gcccagctac ggcgcccggc cggtcagcag cgcggccagc
     4741 gtctatgcag gcgctggggg ctctggttcc cggatctccg tgtcccgctc caccagcttc
     4801 aggggcggca tggggtccgg gggcctggcc accgggatag ccgggggtct ggcaggaatg
     4861 ggaggcatcc agaacgagaa ggagaccatg caaagcctga acgaccgcct ggcctcttac
     4921 ctggacagag tgaggagcct ggagaccgag aaccggaggc tggagagcaa aatccgggag
     4981 cacttggaga agaagggacc ccaggtcaga gactggagcc attacttcaa gatcatcgag
     5041 gacctgaggg ctcagatctt cgcaaatact gtggacaatg cccgcatcgt tctgcagatt
     5101 gacaatgccc gtcttgctgc tgatgacttt agagtcaagt atgagacaga gctggccatg
     5161 cgccagtctg tggagaacga catccatggg ctccgcaagg tcattgatga caccaatatc
     5221 acacgactgc agctggagac agagatcgag gctctcaagg aggagctgct cttcatgaag
     5281 aagaaccacg aagaggaagt aaaaggccta caagcccaga ttgccagctc tgggttgacc
     5341 gtggaggtag atgcccccaa atctcaggac ctcgccaaga tcatggcaga catccgggcc
     5401 caatatgacg agctggctcg gaagaaccga gaggagctag acaagtactg gtctcagcag
     5461 attgaggaga gcaccacagt gctcaccaca cagtctgctg aggttggagc tgctgagacg
     5521 acgctcacag agctgagacg tacagtccag tccttggaga tcgacctgga ctccatgaga
     5581 aatctgaagg ccagcttgga gaacagcctg agggaggtgg aggcccgcta cgccctacag
     5641 atggagcagc tcaacgggat cctgctgcac cttgagtcag agctggcaca gacccgggca
     5701 gagggacagc gccaggccca ggagtatgag gccctgctga acatcaaggt caagctggag
     5761 gctgagatcg ccacctaccg ccgcctgctg gaagatggcg aggactttaa tcttggtgat
     5821 gccttggaca gcagcaactc catgcaaacc atccaaaaga ccaccacccg ccggatagtg
     5881 gatggcaaag tggtgtctga gaccaatgac accaaagttc tgaggcatcg aattctgcag
     5941 tcgacggtac cgcgggcccg ggatccaccg gtcgccacc
//
