LOCUS       sequence-183118-        9238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral tet-inducible expression of miR-722and Dendra 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116."
COMMENT     Sequence Label: miR-722 plix4.03
FEATURES             Location/Qualifiers
     source          1..9238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..50
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     47..71
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1246..1938
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             1945..1962
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        1972..2482
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2401..2419
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2492..3088
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2492..2511)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2988..3008
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3089..3142
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3143..3760,3761..3766,3767..3802,3803..3805,3806..3841,
                     3842..3844,3845..3880,3881..3889)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3929..3949)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4347..4358)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4563..4796
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4868..4989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4905..4924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4959..4978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5029..5164
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5164..5235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5236..5304,5305..6096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5454..5473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6267..6855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6756..6775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7009..7026
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7143..7164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7179..7184,7185..7202,7203..7209)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7217..7233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7222..7244
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7241..7257
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7241..7257
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7276..7296
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7278..7296
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7324..7550
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7551..7731
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7778..7903
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8396..8629
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8814..8858
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9071..9188
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
       61 accgtcagat cgcctggaga attggctagc gccaccatgg atgaggaaat cgctgccctg
      121 gtcgttgaca acggctccgg tatgtgcaaa gccggttttg ctggagatga tgcccctcgt
      181 gctgttttcc cctccattgt tggacgaccc agacatcagg tacatttaat aaaccatcct
      241 aataacatgt taatcatatt gaaccacatt aaactctcat ttgtgcattt tacatctttt
      301 aaaaccgatt tatattcgtt tttagataat gaagtttatt aaagtgttaa ttcacagctt
      361 tattgcttgt tttgagaatc tgcttgcata agtgattttt aaacttgatc tgattttcgt
      421 ttgtttcgaa gttcttcagc tttgaaagtc agatcttcaa ttcacagcca tgccatagtt
      481 ttaggataca gtataagtag acgaattggg atgcatcgta gattttatgc tgtgtttgtt
      541 atggtttttc agtggtagag attttcagag tattattagg gttatagtca gtgtcaggtt
      601 tatttatcgt cagcgtgatg ggcaaaaaac agctggcagt acgggctaat caggactgtg
      661 ttgctgtctg tgtggctgcg cagatgttgg ggacgtccag cggagacctg tgtattggaa
      721 cggagtggaa tttgaaacgt tttggccaaa aatgtagcca tggcaaaggg gttttttgca
      781 gaaacgtttc agatttcgct ccgttctccg gcttcggaaa aaacctgctg ctgccgagag
      841 gaagacgaag aggagccaac tgctgttact ctcgcccaga aatctgcgcc aacatggaca
      901 cacccagtgg agcaagccat ctgattttca tggtcagatt gtaaagaagc aattactctt
      961 ggacagttta agcagaaaag ccaccaagtc aagtcactgg tcatatgatg aactcgaaga
     1021 tctaataaaa gttaatttaa gcgttcatta caataaattc aagtgaatct gttttaagct
     1081 agcctttaac gtttttaaat tccttcaaac aaattagttc ctctttgcag ttcctcattt
     1141 ctcggtgagc tggtgcttta atccctttac tttttcttcc tctagggagt gatggttggc
     1201 atgggacgaa ttaattcctg cagcccgggg gtaccggtcg ccaccatgaa caccccggga
     1261 attaacctga tcaaggagga catgcgcgtg aaggtgcaca tggagggcaa cgtgaacggc
     1321 cacgccttcg tgatcgaggg cgagggcaag ggcaagccct acgagggcac ccagaccgcc
     1381 aacctgaccg tgaaggaggg cgcccccctg cccttcagct acgacatcct gaccaccgcc
     1441 gtgcactacg gcaaccgggt gttcaccaag taccccgagg acatccccga ctacttcaag
     1501 cagagcttcc ccgagggcta cagctgggag cgcaccatga ccttcgagga caagggcatc
     1561 tgcaccatcc gcagcgacat cagcctggag ggcgactgct tcttccagaa cgtgcgcttc
     1621 aagggcacca acttcccccc caacggcccc gtgatgcaga agaaaaccct gaagtgggag
     1681 cccagcaccg agaagctgca cgtgcgcgac ggcctgctgg tgggcaacat caacatggcc
     1741 ctgctgctgg agggcggcgg ccactacctg tgcgacttca agaccaccta caaggccaag
     1801 aaggtggtgc agctgcccga cgcccacttc gtggaccacc gcatcgagat cctgggcaac
     1861 gacagcgact acaacaaggt gaagctgtac gagcacgccg tggcccgcta cagccccctg
     1921 cccagccagg tgtggtaacc ggtccaccac caccaccacc actaaggatc cggggttggg
     1981 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     2041 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     2101 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     2161 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     2221 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     2281 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     2341 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     2401 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     2461 aatcaccgac ctctctcccc agcaattcac catgaccgag tacaagccca cggtgcgcct
     2521 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     2581 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     2641 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     2701 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     2761 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     2821 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     2881 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     2941 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     3001 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     3061 ctggtgcatg acccgcaagc ccggtgccga aggtagaggt tctctcctca cttgtggtga
     3121 tgttgaagaa aaccctggtc caatgtctag actggacaag agcaaagtca taaacggagc
     3181 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     3241 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     3301 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     3361 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     3421 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     3481 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     3541 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     3601 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     3661 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     3721 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     3781 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     3841 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga
     3901 tcgatccaag atatcgtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3961 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4021 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4081 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4141 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4201 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4261 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4321 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4381 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4441 tctccctttg ggccgcctcc ccgcctgttt cgcctcggcg tccggactag aggtaccttt
     4501 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4561 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4621 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4681 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4741 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     4801 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     4861 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4921 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4981 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     5041 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5101 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5161 ggcctttcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5221 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5281 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5341 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5401 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5461 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5521 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5581 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5641 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5701 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5761 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5821 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5881 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5941 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6001 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6061 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6121 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6181 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6241 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6301 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6361 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6421 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6481 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6541 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6601 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6661 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6721 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6781 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6841 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6901 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6961 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7021 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7081 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7141 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7201 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7261 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     7321 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     7381 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     7441 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     7501 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     7561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     7741 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     7801 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     7861 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     7921 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     7981 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     8041 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     8101 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     8161 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     8221 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     8281 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     8341 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     8401 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     8461 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8521 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8581 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     8641 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     8701 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     8761 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     8821 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     8881 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     8941 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9001 ttcagaccca cctcccaacc ccgaggggac aattctcgac ctcgagacaa atggcagtat
     9061 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     9121 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     9181 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcga atcgatat
//
