LOCUS       sequence-18314-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for bacterial expression; His,GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  TITLE     Gateway Destination vectors for E. coli expression
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDest-565
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..708
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="PILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFE
                     LGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGV
                     SRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM
                     DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWXATFGGGDHPPK"
     CDS             715..732
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttaactttta agaaggagat atacatatcg agatcccatc accatcacca tcacggcgcc
       61 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
      121 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
      181 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
      241 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
      301 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
      361 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
      421 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
      481 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
      541 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
      601 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
      661 ttgcagggct ggncagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt
      721 ccgcgtggat ctgatatcac aagtttgtac aaaaag
//
