LOCUS       sequence-183142-        9340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentiviral tet-inducible expression of miRNA and Dendra2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116."
COMMENT     Sequence Label: miR-vector plix4.03
FEATURES             Location/Qualifiers
     source          1..9340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      29..617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     518..537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     771..788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    905..926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(941..946,947..964,965..971)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    979..995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     984..1006
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1003..1019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1003..1019
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1038..1058
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1040..1058
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1086..1312
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1313..1493
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1540..1665
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2158..2391
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2576..2620
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2833..2950
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3004..3318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3012..3030
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3048..3066
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3083..3101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3119..3137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3155..3173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3190..3208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3226..3244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3291..3315
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3334..3343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4307..4999
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             5005..5031
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5047..5064
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        5074..5584
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5503..5521
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5594..6190
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5594..5613)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6090..6110
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6191..6244
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6245..6862,6863..6868,6869..6904,6905..6907,6908..6943,
                     6944..6946,6947..6982,6983..6991)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7031..7051)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7449..7460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7665..7898
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7970..8091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8007..8026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8061..8080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8131..8266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8266..8337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8338..8406,8407..9198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8556..8575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
       61 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      121 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      181 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      241 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      301 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      361 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      421 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      481 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      541 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      601 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      661 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      721 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
      781 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      841 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      901 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      961 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     1021 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     1081 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     1141 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     1201 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     1261 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     1321 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     1381 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     1441 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1501 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     1561 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     1621 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     1681 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     1741 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     1801 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     1861 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     1921 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     1981 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     2041 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     2101 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     2161 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     2221 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     2281 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     2341 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     2401 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     2461 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2521 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2581 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2641 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2701 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2761 gtttcagacc cacctcccaa ccccgagggg acaattctcg acctcgagac aaatggcagt
     2821 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2881 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2941 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc gaatcgatat
     3001 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     3061 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
     3121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     3181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     3241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
     3301 gtgaaccgtc agatcgcctg gagaattggc taggccacca tggatgagga aatcgctgcc
     3361 ctggtcgttg acaacggctc cggtatgtgc aaagccggtt ttgctggaga tgatgcccct
     3421 cgtgctgttt tcccctccat tgttggacga cccagacatc aggtacattt aataaaccat
     3481 cctaataaca tgttaatcat attgaaccac attaaactct catttgtgca ttttacatct
     3541 tttaaaaccg atttatattc gtttttagat aatgaagttt attaaagtgt taattcacag
     3601 ctttattgct tgttttgaga atctgcttgc ataagtgatt tttaaacttg atctgatttt
     3661 cgtttgtttc gaagttcttc agctttgaaa gtcagatctt caattcacag ccatgccata
     3721 gttttaggat acagtataag tagacgaatt gggatgcatc gtagatttta tgctgtgttt
     3781 gttatggttt ttcagtggta gagattttca gagtattatt agggttatag tcagtgtcag
     3841 gtttatttat cgtcagcgtg atgggcaaaa aacagctggc agtacgggct atgtcttcgt
     3901 ctagaagaag acaaagccaa ctgctgttac tctcgcccag aaatctgcgc caacatggac
     3961 acacccagtg gagcaagcca tctgattttc atggtcagat tgtaaagaag caattactct
     4021 tggacagttt aagcagaaaa gccaccaagt caagtcactg gtcatatgat gaactcgaag
     4081 atctaataaa agttaattta agcgttcatt acaataaatt caagtgaatc tgttttaagc
     4141 tagcctttaa cgtttttaaa ttccttcaaa caaattagtt cctctttgca gttcctcatt
     4201 tctcggtgag ctggtgcttt aatcccttta ctttttcttc ctctagggag tgatggttgg
     4261 catgggacga attaattcct gcagcccggg ggtaccggtc gccaccatga acaccccggg
     4321 aattaacctg atcaaggagg acatgcgcgt gaaggtgcac atggagggca acgtgaacgg
     4381 ccacgccttc gtgatcgagg gcgagggcaa gggcaagccc tacgagggca cccagaccgc
     4441 caacctgacc gtgaaggagg gcgcccccct gcccttcagc tacgacatcc tgaccaccgc
     4501 cgtgcactac ggcaaccggg tgttcaccaa gtaccccgag gacatccccg actacttcaa
     4561 gcagagcttc cccgagggct acagctggga gcgcaccatg accttcgagg acaagggcat
     4621 ctgcaccatc cgcagcgaca tcagcctgga gggcgactgc ttcttccaga acgtgcgctt
     4681 caagggcacc aacttccccc ccaacggccc cgtgatgcag aagaaaaccc tgaagtggga
     4741 gcccagcacc gagaagctgc acgtgcgcga cggcctgctg gtgggcaaca tcaacatggc
     4801 cctgctgctg gagggcggcg gccactacct gtgcgacttc aagaccacct acaaggccaa
     4861 gaaggtggtg cagctgcccg acgcccactt cgtggaccac cgcatcgaga tcctgggcaa
     4921 cgacagcgac tacaacaagg tgaagctgta cgagcacgcc gtggcccgct acagccccct
     4981 gcccagccag gtgtggtaac cggttatccg tatgatgtgc cggattatgc gtaaacgcgt
     5041 ccggtccacc accaccacca ccactaagga tccggggttg gggttgcgcc ttttccaagg
     5101 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     5161 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     5221 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     5281 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     5341 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     5401 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     5461 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     5521 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     5581 ccagcaattc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5641 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     5701 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     5761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     5821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     5881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     5941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6181 gcccggtgcc gaaggtagag gttctctcct cacttgtggt gatgttgaag aaaaccctgg
     6241 tccaatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     6301 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     6361 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     6421 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     6481 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     6541 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     6601 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     6661 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     6721 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     6781 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     6841 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     6901 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     6961 cgattttgac cttgacatgc tccccgggta actaagtaag gatcgatcca agatatcgta
     7021 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7081 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7141 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7201 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7261 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7321 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     7381 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7441 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7501 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7561 ccccgcctgt ttcgcctcgg cgtccggact agaggtacct ttaagaccaa tgacttacaa
     7621 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7681 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7741 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7801 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7861 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7921 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8101 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8161 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8221 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctttc aaatatgtat
     8281 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8341 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8401 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8461 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8521 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8581 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8641 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8701 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8761 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8821 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8881 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8941 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9001 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9061 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9121 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9181 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9241 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9301 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
//
