LOCUS       sequence-183161-        7664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with CLDN5 (Ple261) promoter driving expression of 
            iCre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Baumann BH, Shu W, Song Y, Simpson EM, Lakhal-Littleton S, Dunaief 
            JL
  TITLE     Ferroportin-mediated iron export from vascular endothelial cells in 
            retina and brain.
  JOURNAL   Exp Eye Res. 2019 Jul 16:107728. doi: 10.1016/j.exer.2019.107728.
  PUBMED    31323276
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Eye 
            Res. 2019 Jul 16:107728. doi: 10.1016/j.exer.2019.107728."
COMMENT     Sequence Label: pEMS1982
FEATURES             Location/Qualifiers
     source          1..7664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    60..81
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(96..101,102..119,120..126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    134..150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     139..161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     158..174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     158..174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   212..341
                     /label=AAV2 ITR
     intron          3422..3554
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3537..3556
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    4635..4756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4646..4665)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4700..4719
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4925..5054
                     /label=AAV2 ITR
     primer_bind     complement(5073..5090)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5073..5089)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5082..5104)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5231..5686
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5318..5337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5528..5549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5712..5816
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5817..5885,5886..6677)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6035..6054)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6848..7436
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7337..7356
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
       61 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      121 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      181 tacgccagat ttaattaagg ccttaattag gctgcgcgct cgctcgctca ctgaggccgc
      241 ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
      301 gcgcagagag ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc
      361 gccatgctac ttatctacgt agccatgctc taggaagatc ggaattccct aggccggcca
      421 cgcgtttttt ttctcgtttt actcacaaat acttaagtat gtaagtagat acacttcacc
      481 cacataatag agaatactct atacatgtat tcatgggtca aaggggtccc tgagatacac
      541 acaattcata ctcatccctc tgaacactgc aagcgtcgtg tggagccatt tgctttccca
      601 cacactcgct ttctgccgca gcaaggctgg accctgctgg agtggtgatg tgcaggaact
      661 gcctcatagc tcaggtggta aagttatttg gctgggaact gagtctctcc ccacatgctg
      721 tcacaccctt cccggccctg acaaacacaa agagatgcat cccattgagt cacccacttg
      781 gctgaaccgt gtacctggta acctccttgg gaggttcttt ggagggtatt tttctcctct
      841 acacaaagca tgcctgcgaa gtaagctgag aatgacaaaa acaggaaagc ccaatcttgc
      901 agagacagag gggccaaggc ctgcatgata atgcggccca aattcatagc tcagacccaa
      961 aagaaaacaa atgttccatt ctttctgtgt ccacctctaa actaagcaat acagtgaggg
     1021 cagtaaattg caattacaag taagaacttt agcagttgct gatcataaaa tgaaaaggga
     1081 caaatatgtt tatccttaac agggtcacaa ataattccac agctgttaat tttggcagcc
     1141 acgactctgc aggcagtatg ggctcatggt atactatctt cattgtcacc ttggacaaag
     1201 atcctgttgg ccaaaggcac acaggcatct tcaggaaaac tagatttaaa tggtaagatt
     1261 acctatgctt atgtttttaa gctccaaaga ctactggttc tgggaaggac tgggtcacta
     1321 tgtgaccttg tgtaggtctc ttttggccct gagtccagga gggctctgtg actccaggtt
     1381 gtggctgcgt ctggcagcta acaattgttt agtttacagt gtccttcctc ttcatcttgt
     1441 ttgcattcag ttgcccatgc ctgcctctgg ggggaggtgg gaaggaatgt ggctcctggg
     1501 aggagcccag ctccatgtgc cattctgagg agccaggctg cacgcccctg ggccatagct
     1561 gctggtggat ggatgagtac actggcagag gcactgggaa gggaagacag aattgttggc
     1621 caatagtccc cttttactga ttttcttgtc atttttgtgt ttgttttatt ataaagataa
     1681 cagctgtatt tgagaaagaa agctcaaact ataaagaaaa tttaaaattg ccctgtctta
     1741 ctccaactcc attccctgac taggtgagcc agacgctggc cttatctcat ttaccatctc
     1801 agagccatct gaagggggag aagggaaccg ggccccagga gggagaaagt catcaaacct
     1861 cccacatctg tgaccagcct cagtgccata ctttttctat ggagggccct gtccaatgga
     1921 actgagcaca gaccagataa aagaactggg cacccagtgg cctcagtcca gggcctggag
     1981 ttcaaacttt actggaaaca aaggggccga gagagactgg ggaaagaact actagaaagg
     2041 ggctggtgcc cccatggggc tgtgggtttt ggagccgcgt gcccccacct gagcctcagg
     2101 gggcccggag tgtccacacc agtggacctt tcgagaaatg gctgggccat tgtgcagaag
     2161 aatgcccgga aatcccgcgc ctccctcctc cagcaaggat gggggctctt cctcctggcc
     2221 aggaaactcc aagttggctt ccggagggtg gcctgggggc tggggtgcca gggacaccat
     2281 cgccactggt gggagggcag ggcacagccc ctccgtgtcc ctttgtctct cctgtctgaa
     2341 ggccagagca ggctgctagg cctggggcca ccactgcccc tgggtgctac acccagtgtg
     2401 ctgggtcact gggaacttcc tgaagtggtg tcacctgaac tggaccccca aggatggggt
     2461 gcgggcagta ccgcaggaag aggagcagcc cctgtgaaga ttgagaggtc tgggaagccc
     2521 ctgcggcttg ggagagtggg ggtcgccagg cagggggaaa gcccctgtgc caccgctttt
     2581 tgccagagac tcaggctcca gagaggcagt gagtggcatg gggggtgagg ctggggccct
     2641 gggcctgacc tccacacgcc tgcctggcct ctctgtttgc catgggatga gagagacagt
     2701 gctgggactc agagcggggc tggagagtga gagtgcgaga aagggcctgg gtggggcttg
     2761 gaccccgggg cgggctttct ggagagcccc cctacgaggg cctctacggc ggtgacgggg
     2821 tggggggctt ctgcaaacct tggtcaggga agtggagctg gctcgagtgg aagagaccac
     2881 ccggctcagt cggggatgtg ggagtggact gggtggtgca gactgggggt cgagcgcctt
     2941 ctgaagtgac ggggccggga cgcgcaggga ggcggcccaa gaagcgcgcc ctaggccagc
     3001 ccagaatgcg ctcggccgcg actaggacaa cggcgggtgg ggctgggggc ggctgccggg
     3061 cggggagcgg tcccgcgccc tcagctaccc ctcaagagcc gttgtttccc taacttcagc
     3121 tgccagaggc tctgtgattg gctgcggcac gatgacccgc gcacggattg gctgcttcgg
     3181 gccggggggc cgggcccggg ggacagaatc cgcccccgaa ccttcaaaga gggtaccccc
     3241 cggcaggagc tggcagaccc aggaggtgcg acagacccgc ggggcaaacg gactggggcc
     3301 aagagccggg agcgcgggcg caaaggcacc agggcccgcc cagggcgccg cgcagcacgg
     3361 ccttgggggt tctgcgggcc ttcgggtggc gcgccgaagt tggtcgtgag gcactgggca
     3421 ggtaagtatc aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag
     3481 acagagaaga ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc
     3541 ctttctctcc acaggtgtcc actcccaggc ggccgccacc atgtccaacc tgctgactgt
     3601 gcaccaaaac ctgcctgccc tccctgtgga tgccacctct gatgaagtca ggaagaacct
     3661 gatggacatg ttcagggaca ggcaggcctt ctctgaacac acctggaaga tgctcctgtc
     3721 tgtgtgcaga tcctgggctg cctggtgcaa gctgaacaac aggaaatggt tccctgctga
     3781 acctgaggat gtgagggact acctcctgta cctgcaagcc agaggcctgg ctgtgaagac
     3841 catccaacag cacctgggcc agctcaacat gctgcacagg agatctggcc tgcctcgccc
     3901 ttctgactcc aatgctgtgt ccctggtgat gaggagaatc agaaaggaga atgtggatgc
     3961 tggggagaga gccaagcagg ccctggcctt tgaacgcact gactttgacc aagtcagatc
     4021 cctgatggag aactctgaca gatgccagga catcaggaac ctggccttcc tgggcattgc
     4081 ctacaacacc ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg acatctcccg
     4141 caccgatggt gggagaatgc tgatccacat tggcaggacc aagaccctgg tgtccacagc
     4201 tggtgtggag aaggccctgt ccctgggggt taccaagctg gtggagagat ggatctctgt
     4261 gtctggtgtg gctgatgacc ccaacaacta cctgttctgc cgggtcagaa agaatggtgt
     4321 ggctgcccct tctgccacct cccaactgtc cacccgggcc ctggaaggga tctttgaggc
     4381 cacccaccgc ctgatctatg gtgccaagga tgactctggg cagagatacc tggcctggtc
     4441 tggccactct gccagagtgg gtgctgccag ggacatggcc agggctggtg tgtccatccc
     4501 tgaaatcatg caggctggtg gctggaccaa tgtgaacata gtgatgaact acatcagaaa
     4561 cctggactct gagactgggg ccatggtgag gctgctcgag gatggggact gagcggccgc
     4621 gatcgcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     4681 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     4741 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     4801 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtagtc gaaattcccg
     4861 ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt aatcattaac
     4921 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     4981 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     5041 gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca acgtcgtgac
     5101 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     5161 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     5221 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5281 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5341 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5401 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5461 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5521 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5581 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5641 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     5701 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5761 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5821 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5881 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5941 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6001 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6061 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6121 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6181 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6241 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6301 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6361 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6421 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6481 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6541 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6601 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6661 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6721 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6781 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6841 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6901 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6961 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7021 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7081 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7141 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7201 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7261 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7321 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7381 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7441 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7501 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7561 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7621 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcat
//
