LOCUS       sequence-183165-        5790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5790)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Actin-3
FEATURES             Location/Qualifiers
     source          1..5790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2590..2711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2627..2646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2681..2700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2718..3173)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2855..2876)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3067..3086
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3200..3304
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3329..3349)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3373..3583
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3395..3591
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3442..3577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3626..4420
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3626..4420
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3680..3699)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4290..4309
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4608..4627)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4652..4699
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5028..5616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5517..5536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc
      601 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      661 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      721 ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa
      781 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      841 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
      901 aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg
      961 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat
     1021 attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1081 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1141 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1201 cattctggat tgcctgacaa tgccagacga tccggactca gatctcgagc tcaagcttcg
     1261 aattctgcag tcgacggtac cgcgggcccg ggatctggca gcggtggagg cagcgcatcc
     1321 ggcggaagcg gaagcgatga tgatatcgcc gcgctcgtcg tcgacaacgg ctccggcatg
     1381 tgcaaggccg gcttcgcggg cgacgatgcc ccccgggccg tcttcccctc catcgtgggg
     1441 cgccccaggc accagggcgt gatggtgggc atgggtcaga aggattccta tgtgggcgac
     1501 gaggcccaga gcaagagagg catcctcacc ctgaagtacc ccatcgagca cggcatcgtc
     1561 accaactggg acgacatgga gaaaatctgg caccacacct tctacaatga gctgcgtgtg
     1621 gctcccgagg agcaccccgt gctgctgacc gaggcccccc tgaaccccaa ggccaaccgc
     1681 gagaagatga cccagatcat gtttgagacc ttcaacaccc cagccatgta cgttgctatc
     1741 caggctgtgc tatccctgta cgcctctggc cgtaccactg gcatcgtgat ggactccggt
     1801 gacggggtca cccacactgt gcccatctac gaggggtatg ccctccccca tgccatcctg
     1861 cgtctggacc tggctggccg ggacctgact gactacctca tgaagatcct caccgagcgc
     1921 ggctacagct tcaccaccac ggccgagcgg gaaatcgtgc gtgacattaa ggagaagctg
     1981 tgctacgtcg ccctggactt cgagcaagag atggccacgg ctgcttccag ctcctccctg
     2041 gagaagagct acgagctgcc tgacggccag gtcatcacca ttggcaatga gcggttccgc
     2101 tgccctgagg cactcttcca gccttccttc ctgggcatgg agtcctgtgg catccacgaa
     2161 actaccttca actccatcat gaagtgtgac gtggacatcc gcaaagacct gtacgccaac
     2221 acagtgctgt ctggcggcac caccatgtac cctggcattg ccgacaggat gcagaaggag
     2281 atcactgccc tggcacccag cacaatgaag atcaagatca ttgctcctcc tgagcgcaag
     2341 tactccgtgt ggatcggcgg ctccatcctg gcctcgctgt ccaccttcca gcagatgtgg
     2401 atcagcaagc aggagtatga cgagtccggc ccctccatcg tccaccgcaa atgcttctag
     2461 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     2521 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2581 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2701 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     2761 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     2821 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     2881 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     2941 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3001 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3061 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3121 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     3181 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3241 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3301 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     3361 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3421 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3481 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3541 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     3601 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     3661 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3721 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3781 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     3841 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     3901 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     3961 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4021 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4081 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4141 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4201 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4261 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4321 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4381 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     4441 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     4501 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     4561 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     4621 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4681 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4741 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4801 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     4861 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4921 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4981 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5041 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5101 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5161 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5221 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5281 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5341 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5401 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5461 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5521 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5581 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5641 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5701 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     5761 attagttcat agcccatata tggagttccg
//
