LOCUS       sequence-183170-        6444 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6444)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..6444
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..693
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     polyA_signal    2620..2741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2657..2676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2711..2730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2748..3203)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2885..2906)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3097..3116
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3230..3334
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3336..3693
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3336..3527
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3359..3379)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3544..3679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3728..4522
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3728..4522
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3782..3801)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4392..4411
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4710..4729)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4754..4801
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5130..5718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5619..5638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5893..6196
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6197..6400
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6351..6371
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgagcgagc tgatcaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
       61 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      121 atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      181 agcttcatgt acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt
      241 aagcagtcct tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc
      301 gtgctgaccg ctacccagga caccagcttc cagaacggct gcatcatcta caacgtcaag
      361 atcaacgggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac acgcggctgg
      421 gaggccaaca ccgagatgct gtaccccgct gacggcggcc tgagaggcca cagccagatg
      481 gccctgaagc tcgtgggcgg gggctacctg cactgctcct tcaagaccac atacagatcc
      541 aagaaacccg ctaagaacct caagatgccc ggcttccact tcgtggacca cagactggaa
      601 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag
      661 tactgcgacc tccctagcaa actggggcac agagatgaat ccggactcag atctcgagct
      721 caagcttcga tggcgactgc gacccccgtg ccgccgcgga tgggcagccg cgctggcggc
      781 cccaccacgc cgctgagccc cacgcgcctg tcgcggctcc aggagaagga ggagctgcgc
      841 gagctcaatg accggctggc ggtgtacatc gacaaggtgc gcagcctgga gacggagaac
      901 agcgcgctgc agctgcaggt gacggagcgc gaggaggtgc gcggccgtga gctcaccggc
      961 ctcaaggcgc tctacgagac cgagctggcc gacgcgcgac gcgcgctcga cgacacggcc
     1021 cgcgagcgcg ccaagctgca gatcgagctg ggcaagtgca aggcggaaca cgaccagctg
     1081 ctcctcaact atgctaagaa ggaatctgat cttaatggcg cccagatcaa gcttcgagaa
     1141 tatgaagcag cactcaattc gaaagatgca gctcttgcta ctgcacttgg tgacaaaaaa
     1201 agtttagagg gagatttgga ggatctgaag gatcagattg cccagttgga agcctcctta
     1261 gctgcagcca aaaaacagtt agcagatgaa actttactta aagtagattt ggagaatcgt
     1321 tgtcagagcc ttactgagga cttggagttt cgcaaaagca tgtatgaaga ggagattaac
     1381 gagaccagaa ggaagcatga aacgcgcttg gtagaggtgg attctgggcg tcaaattgag
     1441 tatgagtaca agctggcgca agcccttcat gagatgagag agcaacatga tgcccaagtg
     1501 aggctgtata aggaggagct ggagcagact taccatgcca aacttgagaa tgccagactg
     1561 tcatcagaga tgaatacttc tactgtcaac agtgccaggg aagaactgat ggaaagccgc
     1621 atgagaattg agagcctttc atcccagctt tctaatctac agaaagagtc tagagcatgt
     1681 ttggaaagga ttcaagaatt agaggacttg cttgctaaag aaaaagacaa ctctcgtcgc
     1741 atgctgacag acaaagagag agagatggcg gaaataaggg atcaaatgca gcaacagctg
     1801 aatgactatg aacagcttct tgatgtaaag ttagccctgg acatggaaat cagtgcttac
     1861 aggaaactct tagaaggcga agaagagagg ttgaagctgt ctccaagccc ttcttcccgt
     1921 gtgacagtat cccgagcatc ctcaagtcgt agtgtacgta caactagagg aaagcggaag
     1981 agggttgatg tggaagaatc agaggcgagt agtagtgtta gcatctctca ttccgcctca
     2041 gccactggaa atgtttgcat cgaagaaatt gatgttgatg ggaaatttat ccgcttgaag
     2101 aacacttctg aacaggatca accaatggga ggctgggaga tgatcagaaa aattggagac
     2161 acatcagtca gttataaata tacctcaaga tatgtgctga aggcaggcca gactgttaca
     2221 atttgggctg caaacgctgg tgtcacagcc agccccccaa ctgacctcat ctggaagaac
     2281 cagaactcgt ggggcactgg cgaagatgtg aaggttatat tgaaaaattc tcagggagag
     2341 gaggttgctc aaagaagtac agtctttaaa acaaccatac ctgaagaaga ggaggaggag
     2401 gaagaagcag ctggagtggt tgttgaggaa gaacttttcc accagcaggg aaccccaaga
     2461 gcatccaata gaagctgtgc aattatgtag ggatccaccg gatctagata actgatcata
     2521 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2581 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2701 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     2761 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     2821 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     2881 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     2941 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3001 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3061 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3121 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3181 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3241 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3301 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     3361 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3421 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3481 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3541 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3601 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3661 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     3721 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     3781 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     3841 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     3901 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     3961 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4021 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4081 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4141 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4201 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4261 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4321 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4381 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4441 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4501 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4561 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4621 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4681 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     4741 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     4801 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4861 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     4921 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     4981 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5041 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5521 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5761 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5821 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     5881 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     5941 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     6001 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     6061 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     6121 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     6181 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     6241 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     6301 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     6361 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
     6421 ccgctagcgc taccggtcgc cacc
//
