LOCUS       sequence-183202-       11307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HOXA9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, 
            Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu 
            TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley 
            GQ
  TITLE     Haematopoietic stem and progenitor cells from human pluripotent stem
            cells.
  JOURNAL   Nature. 2017 May 17. doi: 10.1038/nature22370.
  PUBMED    28514439
REFERENCE   2  (bases 1 to 11307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 May 17. doi: 10.1038/nature22370."
COMMENT     Sequence Label: pINDUCER-21-HOXA9
FEATURES             Location/Qualifiers
     source          1..11307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(1..19,20..42,43..61,62..84,85..103,104..126,127..145,
                     146..168,169..187,188..210,211..229,230..252,253..271)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        304..342
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     339..363
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    472..496
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             751..768
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(1322..1346)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1399..1425
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1645..2823
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1876..2814
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2771..2791
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2893..2926
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(2933..3550,3551..3556,3557..3592,3593..3595,3596..3631,
                     3632..3634,3635..3670,3671..3679)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3717..4290
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3884..3901)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4111..4130
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4306..4308,4309..4311,4312..5025)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4306..4308,4309..4311,4312..5022)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4351..4372)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4612..4631)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4959..4980
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5161..5180
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5468..6056
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5521..5541)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5939..5950)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6131..6364
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6483..6501)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6569..6673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6674..6742,6743..7534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6892..6911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7705..8293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8194..8213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8447..8464
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8704..8725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8740..8745,8746..8763,8764..8770)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8778..8794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8783..8805
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8802..8818
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8802..8818
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8868..8887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9079..9712
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9759..9884
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10381..10614
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10799..10843
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11136..11253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt
      301 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      361 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      421 agcctccgcg gccccgaact agtccagtgt ggtggaattc tgcagatatc aacaagtttg
      481 tacaaaaaag caggctccac catggccacc actggggccc tgggcaacta ctacgtggac
      541 tcgttcctgc tgggcgccga cgccgcggat gagctgagcg ttggccgcta tgcgccgggg
      601 accctgggcc agcctccccg gcaggcggcg acgctggccg agcaccccga cttcagcccg
      661 tgcagcttcc agtccaaggc gacggtgttt ggcgcctcgt ggaacccagt gcacgcggcg
      721 ggcgccaacg ctgtacccgc tgcggtgtac caccaccatc accaccaccc ctacgtgcac
      781 ccccaggcgc ccgtggcggc ggcggcgccg gacggcaggt acatgcgctc ctggctggag
      841 cccacgcccg gtgcgctctc cttcgcgggc ttgccctcca gccggcctta tggcattaaa
      901 cctgaaccgc tgtcggccag aaggggtgac tgtcccacgc ttgacactca cactttgtcc
      961 ctgactgact atgcttgtgg ttctcctcca gttgatagag aaaaacaacc cagcgaaggc
     1021 gccttctctg aaaacaatgc tgagaatgag agcggcggag acaagccccc catcgatccc
     1081 aataacccag cagccaactg gcttcatgcg cgctccactc ggaaaaagcg gtgcccctat
     1141 acaaaacacc agaccctgga actggagaaa gagtttctgt tcaacatgta cctcaccagg
     1201 gaccgcaggt acgaggtggc tcgactgctc aacctcaccg agaggcaggt caagatctgg
     1261 ttccagaacc gcaggatgaa aatgaagaaa atcaacaaag accgagcaaa agacgagtag
     1321 gacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta
     1381 gagggcccgc ggttcgaata cccatacgac gtcccagact acgcttagta atgattaatt
     1441 aaactagaaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagtaaccca
     1501 cccaagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     1561 gcgagccgcg taagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     1621 tcctcacggc gagcctagtc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     1681 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     1741 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     1801 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     1861 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     1921 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     1981 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     2041 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     2101 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     2161 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     2221 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     2281 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     2341 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     2401 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     2461 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     2521 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     2581 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     2641 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     2701 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     2761 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     2821 tgaagcggcc gccatgctag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     2881 ttagacggat ccataacttc gtataatgta tgctatacga agttatgcca ccatgtctag
     2941 actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga
     3001 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     3061 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     3121 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     3181 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     3241 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     3301 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     3361 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     3421 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga
     3481 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     3541 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     3601 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     3661 tgacatgctc cccgggtaac taagtaagga tccgcggccg cactagagga attccgcccc
     3721 tctccccccc ccncccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     3781 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     3841 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     3901 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     3961 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     4021 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     4081 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     4141 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     4201 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     4261 gtggttttcc tttgaaaaac acgatgataa gcttaccggt ccaccatggt gagcaagggc
     4321 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4381 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4441 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4501 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4561 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4621 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4681 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4741 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     4801 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4861 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4921 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4981 accgccgccg ggatcactct cggcatggac gagctgtaca agtaagcatg cccgacggcg
     5041 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5101 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5161 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5221 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5281 agttcttctg atgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     5341 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5401 aaaatgaatg caattgttgt tgtttagtcc ctcccaattc gatatcaagc ttatcgcgat
     5461 agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5521 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5581 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5641 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5701 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5761 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5821 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     5881 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5941 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6001 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga
     6061 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6121 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6181 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6241 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6301 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6361 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6421 tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa
     6481 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     6541 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6601 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6661 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6721 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6781 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6841 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6901 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     6961 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7021 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7081 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7141 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7201 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7261 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7321 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7381 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7441 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7501 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7561 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7621 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7681 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7741 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7801 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7861 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7921 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7981 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8041 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8101 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8161 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8221 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8281 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8341 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8401 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8461 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     8521 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8581 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8641 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8701 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8761 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     8821 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8881 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     8941 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     9001 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     9061 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     9121 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     9181 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     9241 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     9301 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     9361 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     9421 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     9481 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     9541 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9601 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9661 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     9721 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     9781 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     9841 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     9901 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     9961 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    10021 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    10081 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    10141 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    10201 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
    10261 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    10321 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    10381 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    10441 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    10501 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    10561 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    10621 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    10681 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    10741 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    10801 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    10861 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    10921 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    10981 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
    11041 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
    11101 tcgccgaatt cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
    11161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    11221 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    11281 ccagtttgga ctaggatcct ttaccac
//
