LOCUS       sequence-183244-        8120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Maf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Mi D, Li Z, Lim L, Li M, Moissidis M, Yang Y, Gao T, Hu TX, Pratt T,
            Price DJ, Sestan N, Marin O
  TITLE     Early emergence of cortical interneuron diversity in the mouse 
            embryo.
  JOURNAL   Science. 2018 Feb 22. pii: science.aar6821. doi: 
            10.1126/science.aar6821.
  PUBMED    29472441
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 22. pii: science.aar6821. doi: 10.1126/science.aar6821."
COMMENT     Sequence Label: RV-Cag-Dio-EYFP-P2A-cMaf
FEATURES             Location/Qualifiers
     source          1..8120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     331..353
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     415..434
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(477..510)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(561..594)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1155..1172)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      1712..1721
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1731..1787)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(1803..2513,2514..2516,2517..2519))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1845..1866)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2194..2213
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2453..2474
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2516..2525
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2537..2570
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    2621..2654
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2664..3252
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2717..2737)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3135..3146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3447..3916
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4046..4063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4217..4805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4297..4316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4965..5756,5757..5825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5588..5607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        5977..6356
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6357..6560
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6511..6531
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6561..6736
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    6799..6998
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7199..7615
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7545..7567
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7583..7599
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        7629..8008
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
       61 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
      121 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
      181 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
      241 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
      301 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
      361 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
      421 tgctggttat tgtgctgtct catcattttg gcaaagaatt cgctagcgga tccggaataa
      481 cttcgtatag gatactttat acgaagttat gcagaatggt agctggattg tagctgctat
      541 tagcaatatg aaacctctta ataacttcgt atagcataca ttatacgaag ttatttaatt
      601 aactacatga agaactcggg gctgctaggg ttgtcgctgg agctgccgtt ctctctgaag
      661 ccgttggaca ccagcttttc gtacttctct ttgtaggcgt ccctttcgcg cacgagtctg
      721 ctgatctctt gcttcaggtg gtcgacctgc tgcagaagct ggttcttctc gctttccagc
      781 acgtgtctct gctgcactct cttgaatctg cagctctggg cgtagcctct gttcttcagg
      841 gtccgccttt tctgcttcag ccggatcact tcttctttgg acacgcctct cagctgtctg
      901 ttcagctcgc gcacgctcat ggtgaccagc tgctcgtcag agaatctgtc gtcgaagtgc
      961 agtccgcctg cagcatgatg agggtgaagt gctccgcctg caccagctgt tcctccgcca
     1021 cctccgccgc caccgccacc accggcagat gcaggtcctc cgccgccagc tccgcctgct
     1081 ccgctagcag aagcagaagc tccgccagca gcacctggag caccagctgt aggatgatga
     1141 tggtggccgg cagcatggtg atggtggtgg tgatagtgag gagcagcgcc agactgtgca
     1201 gcagctgcag caatcacagc agacaccaca gcagcggcag gtcccatttc ctcgccagat
     1261 cctccaagag aagcaccagc accagcgcct gcagcagcag ccagctgctg tgcgcctcta
     1321 gcatatccat cgaagccgcc ttgcagctgg tggctgttgc tgatcagggc ttccacagca
     1381 tcctcagggc taaagcccag agcctcaggg ttcagctgct gagggtagcc tgtcatccag
     1441 tagtaatctt ccaggtgagc cttctgctcg ctgccagagc caggagatgg agcagagaag
     1501 cttggagaag gtggcacgct gctgcaaggt gtgctcatag gggtgctgct cagagatccg
     1561 ccagcgatca gtctgccgca ctggctgatg attctgtctg tctcgacggg ctctttcttc
     1621 acttcgaact tcatcaggtc gaagtcgttc acgtattcca tggccagagg gcttgtaggc
     1681 aggtcgctgt tgttcatggc cagttcagat gccatggtgg cctccggtcc aggcccgggg
     1741 ttttcttcaa catcacctgc ttgctttagc agagagaagt ttgtggcgcc ggatcctctg
     1801 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     1861 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta gctcaggtag
     1921 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     1981 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     2041 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     2101 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     2161 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     2221 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     2281 ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt agccgaaggt ggtcacgagg
     2341 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     2401 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     2461 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
     2521 gtggcggcgc gccgcgataa cttcgtataa agtatcctat acgaagttat ttgccttaac
     2581 ccagaaatta tcactgttat tctttagaat ggtgcaaaga ataacttcgt ataatgtatg
     2641 ctatacgaag ttatgtttaa accaatcaac ctctggatta caaaatttgt gaaagattga
     2701 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2761 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2821 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2881 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2941 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3001 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     3061 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3121 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3181 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3241 ccgcctcccc gcctggaatt cgagctcgag cttgttaaca tcgataaaat aaaagatttt
     3301 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     3361 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
     3421 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     3481 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     3541 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     3601 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     3661 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     3721 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     3781 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     3841 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     3901 cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
     3961 tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4021 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4621 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4861 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc
     4921 cgcaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4981 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5041 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5101 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5161 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5221 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5281 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5341 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5401 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5461 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5521 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5581 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5641 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5701 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5761 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5821 ctcatactct tcctttttca atgatccggc cattagccat attattcatt ggttatatag
     5881 cataaatcaa tattggctat tggccattgc atacgttgta tccatatcat aatatgtaca
     5941 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
     6001 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     6061 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     6121 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     6181 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     6241 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     6301 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     6361 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     6421 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     6481 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     6541 aggtctatat aagcagagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     6601 ctccgattga ctgagtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca
     6661 tccgaatcgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccacg
     6721 acgggggtct ttcatttggg ggctcgtccg ggatttggag acccctgccc agggaccacc
     6781 gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt
     6841 gtctatgttt gatgttatgc gcctgcgtct gtactagtta gctaactagc tctgtatctg
     6901 gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc
     6961 cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc
     7021 gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc
     7081 gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg
     7141 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg
     7201 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     7261 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     7321 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     7381 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     7441 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     7501 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     7561 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat
     7621 tcgatgtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     7681 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     7741 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     7801 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     7861 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     7921 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     7981 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     8041 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     8101 tgcagcgatg ggggcggggg
//
