LOCUS       sequence-18325-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Shin DH, Lou Y, Jancarik J, Yokota H, Kim R, Kim SH
  TITLE     Crystal structure of TM1457 from Thermotoga maritima.
  JOURNAL   J Struct Biol. 2005 Nov . 152(2):113-7.
  PUBMED    16242963
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 2005 Nov . 152(2):113-7."
COMMENT     Sequence Label: pHM6g.TM1457
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             374..391
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(444..462)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      458..505
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(629..648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctttacttc cgggccatag cggcggcggc ggcggcggcc atatgataaa agtaactgtg
       61 actaattctt tttttgaagt aacgggtcat gcaccagaca aaacactgtg cgcttcagtt
      121 agtcttttga cgcagcatgt ggcgaacttt ctgaaagccg agaaaaaagc gaaaataaag
      181 aaggaatccg gttatctgaa agtgaaattt gaagaactcg aaaactgcga agtgaaggtg
      241 ctagcggcta tggtgaggtc tttgaaagaa ctcgaacaga agtttccttc tcagatcaga
      301 gtggaggtga tcgacaatgg ctcataagga tccgaattcg agctccgtcg acaagcttgc
      361 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      421 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      481 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      541 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      601 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      661 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      721 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtantggg
      781 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacg
//
