LOCUS       sequence-183255-       10017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Accessory Plasmid for PACE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Packer MS, Rees HA, Liu DR
  TITLE     Phage-assisted continuous evolution of proteases with altered 
            substrate specificity.
  JOURNAL   Nat Commun. 2017 Oct 16;8(1):956. doi: 10.1038/s41467-017-01055-9.
  PUBMED    29038472
REFERENCE   2  (bases 1 to 10017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-16"; volume: "8(1):956. doi"; pages: " 
            10.1038/s41467-017-01055-9"
COMMENT     Sequence Label: MSP832
FEATURES             Location/Qualifiers
     source          1..10017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..951)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      999..1221
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     1785..1804
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1985..3259
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             3259..4338
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             4402..5382
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     CDS             complement(8212..8664)
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDRG"
     terminator      complement(8849..8895)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(8946..9737,9738..9806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9569..9588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9807..9911)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9979..9997
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
     1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctactaatac gactcactat
     1801 agggagaaag aaggagcgac attgctccgt gtattcactc gttggaatga atacacagtg
     1861 cagtgtttat tctgttattt atgccaaaaa taaaggccac tatcaggcag ctttgttgtt
     1921 ctgtttacca agttcaggag gtaactcata agaaagacct gcaggtgcag taaaggaaaa
     1981 aaaaatgaaa aaattattat tcgcaattcc tttagttgtt cctttctatt ctcactccgc
     2041 tgaaactgtt gaaagttgtt tagcaaaacc ccatacagaa aattcattta ctaacgtctg
     2101 gaaagacgac aaaactttag atcgttacgc taactatgag ggctgtctgt ggaatgctac
     2161 aggcgttgta gtttgtactg gtgacgaaac tcagtgttac ggtacatggg ttcctattgg
     2221 gcttgctatc cctgaaaatg agggtggtgg ctctgagggt ggcggttctg agggtggcgg
     2281 ttctgagggt ggcggtacta aacctcctga gtacggtgat acacctattc cgggctatac
     2341 ttatatcaac cctctcgacg gcacttatcc gcctggtact gagcaaaacc ccgctaatcc
     2401 taatccttct cttgaggagt ctcagcctct taatactttc atgtttcaga ataataggtt
     2461 ccgaaatagg cagggggcat taactgttta tacgggcact gttactcaag gcactgaccc
     2521 cgttaaaact tattaccagt acactcctgt atcatcaaaa gccatgtatg acgcttactg
     2581 gaacggtaaa ttcagagact gcgctttcca ttctggcttt aatgaggatc cattcgtttg
     2641 tgaatatcaa ggccaatcgt ctgacctgcc tcaacctcct gtcaatgctg gcggcggctc
     2701 tggtggtggt tctggtggcg gctctgaggg tggtggctct gagggtggcg gttctgaggg
     2761 tggcggctct gagggaggcg gttccggtgg tggctctggt tccggtgatt ttgattatga
     2821 aaagatggca aacgctaata agggggctat gaccgaaaat gccgatgaaa acgcgctaca
     2881 gtctgacgct aaaggcaaac ttgattctgt cgctactgat tacggtgctg ctatcgatgg
     2941 tttcattggt gacgtttccg gccttgctaa tggtaatggt gctactggtg attttgctgg
     3001 ctctaattcc caaatggctc aagtcggtga cggtgataat tcacctttaa tgaataattt
     3061 ccgtcaatat ttaccttccc tccctcaatc ggttgaatgt cgcccttttg tctttggcgc
     3121 tggtaaacct tacgagttca gtatcgactg cgataagatc aacctgttcc gcggtgtctt
     3181 tgcgtttctt ttatatgttg ccacctttat gtatgtattt tctacgtttg ctaacatact
     3241 gcgtaataag gagtcttaat gaaatttgga aactttttgc ttacatacca acctccccaa
     3301 ttttcccaaa cagaggtaat gaaacgtttg gttaaattag gtcgcatctc tgaggagtgt
     3361 ggttttgata ccgtatggtt actggagcat catttcacgg agtttggttt gcttggtaac
     3421 ccttatgtcg ctgctgcata tttacttggc gcgactaaaa aattgaatgt aggaactgcc
     3481 gctattgttc ttcccacagc ccatccagta cgccaacttg aagatgtgaa tttattggat
     3541 caaatgtcaa aaggacgatt tcggtttggt atttgccgag ggctttacaa caaggacttt
     3601 cgcgtattcg gcacagatat gaataacagt cgcgccttag cggaatgctg gtacgggctg
     3661 ataaagaatg gcatgacaga gggatatatg gaagctgata atgaacatat caagttccat
     3721 aaggtaaaag taaaccccgc ggcgtatagc agaggtggcg caccggttta tgtggtggct
     3781 gaatcagctt cgacgactga gtgggctgct caatttggcc taccgatgat attaagttgg
     3841 attataaata ctaacgaaaa gaaagcacaa cttgagcttt ataatgaagt ggctcaagaa
     3901 tatgggcacg atattcataa tatcgaccat tgcttatcat atataacatc tgtagatcat
     3961 gactcaatta aagcgaaaga gatttgccgg aaatttctgg ggcattggta tgattcttat
     4021 gtgaatgcta cgactatttt tgatgattca gaccaaacaa gaggttatga tttcaataaa
     4081 gggcagtggc gtgactttgt attaaaagga cataaagata ctaatcgccg tattgattac
     4141 agttacgaaa tcaatcccgt gggaacgccg caggaatgta ttgacataat tcaaaaagac
     4201 attgatgcta caggaatatc aaatatttgt tgtggatttg aagctaatgg aacagtagac
     4261 gaaattattg cttccatgaa gctcttccag tctgatgtca tgccatttct taaagaaaaa
     4321 caacgttcgc tattatatta tggcggtggc ggtagcggcg gtggcggtag cggcggtggc
     4381 ggtagcggcg gtggcggtag caaatttgga ttgttcttcc ttaacttcat caattcaaca
     4441 actgttcaag aacagagtat agttcgcatg caggaaataa cggagtatgt tgataagttg
     4501 aattttgaac agattttagt gtatgaaaat catttttcag ataatggtgt tgtcggcgct
     4561 cctctgactg tttctggttt tctgctcggt ttaacagaga aaattaaaat tggttcatta
     4621 aatcacatca ttacaactca tcatcctgtc cgcatagcgg aggaagcttg cttattggat
     4681 cagttaagtg aagggagatt tattttaggg tttagtgatt gcgaaaaaaa agatgaaatg
     4741 cattttttta atcgcccggt tgaatatcaa cagcaactat ttgaagagtg ttatgaaatc
     4801 attaacgatg ctttaacaac aggctattgt aatccagata acgattttta tagcttccct
     4861 aaaatatctg taaatcccca tgcttatacg ccaggcggac ctcggaaata tgtaacagca
     4921 accagtcatc atattgttga gtgggcggcc aaaaaaggta ttcctctcat ctttaagtgg
     4981 gatgattcta atgatgttag atatgaatat gctgaaagat ataaagccgt tgcggataaa
     5041 tatgacgttg acctatcaga gatagaccat cagttaatga tattagttaa ctataacgaa
     5101 gatagtaata aagctaaaca agagacgcgt gcatttatta gtgattatgt tcttgaaatg
     5161 caccctaatg aaaatttcga aaataaactt gaagaaataa ttgcagaaaa cgctgtcgga
     5221 aattatacgg agtgtataac tgcggctaag ttggcaattg aaaagtgtgg tgcgaaaagt
     5281 gtattgctgt cctttgaacc aatgaatgat ttgatgagcc aaaaaaatgt aatcaatatt
     5341 gttgatgata atattaagaa gtaccacacg gaatatacct aaacttaatt aacggcactc
     5401 ctcagcaaat ataatgaccc tcttgataac ccaagagggc attttttaat gcccatggcg
     5461 tcatgttgaa aatctccatt acgcgaacgc gaagtccgac tctaagatgt cacggaggtt
     5521 caagttacct ttagccggaa gtgctggcat tttgtccaat tgagactcgt gcaactggtc
     5581 agcgaactgg tcgtagaaat cagccagtac atcacaagac tcatatgtgt caaccatagt
     5641 ttcgcgcact gctttgaaca ggttcgcagc gtcagccgga atggtaccga aggagtcgtg
     5701 aatcagtgca aaagattcga ttccgtactt ctcgtgtgcc cacactacag tcttacgaag
     5761 gtggctaccg tcttggctgt gtacaaagtt aggagcgata ccagactcct gtttgtgtgc
     5821 atcaatctcg ctatctttgt tggtgttaat ggtaggctgt aagcggaact gaccgaggaa
     5881 catcaggttc aagcgcgtct gaataggctt cttgtattcc tgccacacag ggaaaccatc
     5941 aggagttacc caatgcacag cgcaacgctt gcgaagaatc tctccagtct tcttatcttt
     6001 gacctcagca gccagcagct tagcagcaga cttaagccag ttcattgctt caaccgcagc
     6061 taccaccgtc acgctcacag attcccaaat cagcttagcc atgtatccag cagcctgatt
     6121 cggctgagtg aacatcagac ccttgccgga atcaatagct ggctgaatgg tatcttccag
     6181 cactgattga cggaagccga actctttgga cccgtaagcc agcgtcatga ctgaacgctt
     6241 agtcacactg cgagtaacac cgtaagccag ccattgacca gccagtgcct tagtgcccag
     6301 cttgactttc tcagagattt caccagtgtt ctcatcggtc acggtaacta cttcgttatc
     6361 ggtcccattg attgcgtctg cttgtagaat ctcgttgact ttcttagcaa caatcccgta
     6421 gatgtcctga acggtttcac taggaagcaa gttaaccgcg cgaccaccta cctcatctcg
     6481 gagcatcgcg gagaagtgct ggatgccaga gcaagacccg tcaaacgcca gcggaaggga
     6541 gcagttatag ctcaggccgt ggtgctgtac cccagcgtac tcaaagcaga acgcaaggaa
     6601 gcagaacgga gaatcttgct cagcccacca agtgttctcc agtggagact tagcgcaagc
     6661 catgatgttc tcgtggtttt cctcaatgaa cttgatgcgc tcagggaacg gaaccttatc
     6721 gacacccgca cagtttgcac cgtggatttt cagccagtag taaccttcct taccgattgg
     6781 tttacctttc gccagcgtaa gcagtccttt ggtcatatcg ttaccttgcg ggttgaacat
     6841 tgacacagcg taaacacgac cgcgccagtc catgttgtaa gggaaccaga tggccttatg
     6901 gttagcaaac ttattggctt gctcaagcat gaactcaagg ctgatacggc gagacttgcg
     6961 agccttgtcc ttgcggtaca cagcagcggc agcacgtttc cacgcggtga gagcctcagg
     7021 attcatgtcg atgtcttccg gtttcatcgg gagttcttca cgctcaatcg cagggatgtc
     7081 ctcgaccgga caatgcttcc acttggtgat tacgttggcg accgctagga ctttcttgtt
     7141 gattttccat gcggtgtttt gcgcaatgtt aatcgctttg tacacctcag gcatgtaaac
     7201 gtcttcgtag cgcatcagtg ctttcttact gtgagtacgc accagcgcca gaggacgacg
     7261 accgttagcc caatagccac caccagtaat gccagtccac ggcttaggag gaactacgca
     7321 aggttggaac atcggagaga tgccagccag cgcacctgca cgggttgcga tagcctcagc
     7381 gtattcaggt gcgagttcga tagtctcaga gtcttgacct actacgccag cattttggcg
     7441 gtgtaagcta accattccgg ttgactcaat gagcatctcg atgcagcgta ctcctacatg
     7501 aatagagtct tccttatgcc acgaagacca cgcctcgcca ccgagtagac ccttagagag
     7561 catgtcagcc tcgacaactt gcataaatgc tttcttgtag acgtgcccta cgcgcttgtt
     7621 gagttgttcc tcaacgtttt tcttgaagtg cttagcttca aggtcacgga tacgaccgaa
     7681 gcgagcctcg tcctcaatgg cccgaccgat tgcgcttgct acagcctgaa cggttgtatt
     7741 gtcagcactg gttaggcaag ccagagtggt cttaattgtg atgtacgcta cggcttccgg
     7801 cttgatttct tgcaggaact ggaaggctgt cgggcgcttg ccgcgcttag ctttcacttc
     7861 ctcaaaccag tcgttgatgc gtgcaatcat cttagggagt agggtagtga tgagaggctt
     7921 agcggcagcg ttatccgcaa cctcaccagc tttaagttga cgctcaaaca tcttgcggaa
     7981 gcgtgcttca cccatctcgt aagactcatg ctcaagggcc aactgttcgc gagctaaacg
     8041 ctcaccgtaa tggtcagcca gagtgttgaa cgggatagca gccagttcga tgtcagagaa
     8101 gtcgttctta gcgatgttaa tcgtgttacc gcccgctcca ctgcctgcgc taccgcctgc
     8161 catgtgacca accagggggt gacccgcacc accactcgca ccaccgccgc taccacggtc
     8221 agaagtgacc agttcgttct tctcccacca acgcttaagg tcgaacgaag gggaagcctt
     8281 cggcgccacc tcatgatggg cgcgaagcac agcgccttcg tacttagcca gcagtgtgac
     8341 aagcagtgag cgaagggatt gcatttgggc tggcgtaaag ttagcgtcga acttaccttt
     8401 atcgtcgata ccaccaacaa ggcagacgcc gatagagttg tggttgtaac ccttagcgtg
     8461 agagcctaca gccatctcat ctcgtcctgc ctccacagta ccgtctcgct tgatgataaa
     8521 gtggtatccc acatcgagcc aaccctgctc tttgtgccac tggcgaatct cacggacacc
     8581 aacattctga cttggcttgg tagccgagca gtgaacaaag attgcgtcag tagattcacg
     8641 ttgtttaaac tgtacacgag ccattttttt tttcctttcg aggaaggaac tctagtaaaa
     8701 gttaaacaaa attatttgta gagggaaacc gttgtggtct ccctgaatat atattacgag
     8761 ccttatgcat gcccgtaaag ttatccagca accactcata gacctagggc agcagatagg
     8821 gacgacgtgg tgttagctgt gctctagaat aaaacgaaag gcccagtctt tcgactgagc
     8881 ctttcgtttt atttgaccgg aaatcagaat tggttaattg gttgtaacac tggcagagca
     8941 ttacgttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9241 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9541 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9901 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9961 atgacattaa cctataaaaa taggcgtatc acgaggccct taggccaaat aggccgt
//
