LOCUS       sequence-183258-        7030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  It contains ~2.7 Kb of the miR-183-96-182 cluster promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Segura MF, Jubierre L, Li S, Soriano A, Koetz L, Gaziel-Sovran A, 
            Masanas M, Kleffman K, Dankert JF, Walsh MJ, Hernando E
  TITLE     Kruppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster 
            under physiologic and pathologic conditions.
  JOURNAL   Oncotarget. 2017 Apr 18;8(16):26298-26311. doi: 
            10.18632/oncotarget.15459.
  PUBMED    28412746
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget"; journalName: "Oncotarget"; date: "2017-04-18-
            18"; volume: "8"; issue: "16"; pages: "26298-26311"
COMMENT     Sequence Label: pGL4-Cluster Promoter Delta 3
FEATURES             Location/Qualifiers
     source          1..7030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..143
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     92..111
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2967..2998
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3025..3034
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3031..4683
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4727..4848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4738..4757)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4792..4811
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5096..5113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5267..5855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5347..5366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6055..6846,6847..6915))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    join(7020..7030,1..38)
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
       61 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
      121 tgcaggtgcc agaacatttc tctggcctaa ctggccggta cctgagctcg ctagcctcga
      181 ggataacggt cttcaagtgc aagggttagg tttggtggga gcataggcta agcaccaccc
      241 tcattttaca ggggcttcta gggtgccaga tctgcggggg tcgattggga agaactagaa
      301 gctaaaaagg agaagggcgg attaacgttt tattttaatc tggttttgag ctgccgttgc
      361 cgtctggggc ggccgggcgg ggcggagctg ggactgccaa gggcagccaa agcagcaggg
      421 agggaggggg cgctccggtg ctcaggatgc tggggaagaa ggaagaggcc accccaacgc
      481 tgacagcttg gaggacctag cccctgcccc ccggtccctt ctgaatgctc cgggtggtgt
      541 cagaccaaag gacggttgag aaggatctaa tccgcgtccg cgccccagcc ccggctggcc
      601 ccgccccctc ccgagggagc cactgtcgca gtggggggag ggggagatgg gaacgggtct
      661 ggggggcggc tcccctcccc ctccctctct tgggacagac agcctttgtt cccggcggcg
      721 gcggcagcgg tggccagaca cctgtgaggc cgcagaactg gccgagggtg ggcgtgtgag
      781 gacatggagg ccccttgtcc ccattccagc cctgcgcccc tgggtccggg tgcgccccct
      841 gagacaggtc agccttgagc acctttctgc ggggagactg cagagatcgt ccagtggccc
      901 tacggtgcgc cccagctccc aacgcacttc ccctcccccc tcccagggtc ggatttccaa
      961 gcacgccacg ccacacacac ctcggctagc tccgggctca gagggcgctg gggtggcagg
     1021 gcgtccgggc gcacctcggc tagcggcacc ctagcccgag ggcaggaggc ggggatagaa
     1081 ggcgggcgac gggacccctt ggggccagac ccggccgggt tgggcaagca ggcggggctc
     1141 ccggcgtctc cgcagtcccg gacgggcgtg ccctgcgccg cgcaccaacc ggagccacat
     1201 tcaaggtgaa tcatccccgg cccgccccct gcccctcccc gcctcgcggc acctgagcca
     1261 atggcgctgc gcgaggggcg ggcggggccc ggggcggggg gcggtaaaac cctcctggag
     1321 tcattctctg tgcactccgt cgagcccccg cgcagccgtg ccggagccgc agggcctccg
     1381 tccagccgcg cggtccgggc agcccgcgcc ccctgttctt ccggtcctcg ggcccaggta
     1441 cccctctgga cctcccccgc ctgcagcgcg gaggcggctc ctcttcaggt ggccgggccg
     1501 gggccttccc cggcagacct gtcacccgac acccaggaag cgcgcggccg ggtcccggct
     1561 cccctttgtt cccccgctgg gctcggcgct ccggcgccgc cccctctcgc ggggcagcgg
     1621 aggtttgggc ccgggccggg cgcgcttcaa gctccgtcca tgcggctctt tccaaaccgg
     1681 ggccgttttt gcctggcttc tctggtgtgg actccgcgcg gagctgagag gcggctgtgc
     1741 gcccagcctg gagccggcgc aggtgagtgt ctggggccgg gagcgggggg gcgggccggg
     1801 cggagccaga gcggggctca gtagaggagg cggaggcggg cgcccgggac cggccgcact
     1861 agaacccggg tgcagggcag aagtcccggc gcagctggct ttcggggatg acgcggcagt
     1921 gcctactcgc tcttccgcgc cttcaggcgg aggcgcccac ggggcgcctg ggtcccgggt
     1981 ccgccagtgg cgtctgccca gcgcccagcg ctgttcccgg gcgggggcgg gggcgggagc
     2041 ggctgcggtc cgagtgcggc cggtgcttgc gaggtgccta cccgcgagga gtttggctgg
     2101 ggtcgggctg cgtttcgccg ccctggtggg ctcaggcgga agcgccaggc tgggaggggg
     2161 ctgcggagag cgccggggcc ccggagctgg cccctccagc gtcttgtcag ggctgcgcgg
     2221 ctggttcgag gctgcttccg tggcccggag ccgccccgcc cagtctctgg gggaaagcgg
     2281 ctctcagacc tcccgtgtgc ctccacttcg cggccactca tcccgggaac cccggcgcac
     2341 gcgaatgaga caccgttccc cgccctcaac tgccccattc ctgttaatag cgctagtggc
     2401 acccaggttc ccagcccttc cccaagatcg gcggctggac ggcttgcctc tggcgcctca
     2461 ccggtcagcg catcccagga ctatgggcag tgagggtgtc acccggggtc aggggtcgcc
     2521 cgattccacg gtgtcctcca ggctgagcca ttcgtttgct cttccaaccc gggctcccgg
     2581 acaccgtgta cagtaatacc cgccgggggc aggggcactg aggccggctc tgagcgatta
     2641 gggccgaggg cgggcggtgc cgtaatcgac ctccccaccc ccacaggcaa ggtgcggcgg
     2701 cgcctcccag gtttgcgccc gtcttcgcaa agctcctgag ggcgaaaatc ctgcgagcca
     2761 ggtggcggat tctcgggccg ggtcactcag ctctacgggt gcgcatcacc ctctacctgt
     2821 cacccagtga gcccgaccga tgggcaccga gtcggcggat ctctgatccg aggccgagcc
     2881 ttcggagaaa tctgtcctag tggcctcatc gtaagggcga attctgcaga tatcaagatc
     2941 tggcctcggc ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct
     3001 tggcaatccg gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc
     3061 ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg
     3121 aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac
     3181 attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat
     3241 gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg
     3301 cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac
     3361 gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag
     3421 aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc
     3481 atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc
     3541 catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa
     3601 accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta
     3661 ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag
     3721 atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc
     3781 accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag
     3841 gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca
     3901 ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac
     3961 gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc
     4021 ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg
     4081 atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag
     4141 gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg
     4201 tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct
     4261 ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag
     4321 cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc
     4381 ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc
     4441 ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt
     4501 aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag
     4561 aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg
     4621 gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg
     4681 taataattct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     4741 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4801 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4861 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     4921 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
     4981 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     5041 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
     5101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5701 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6001 tatatgagta aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca
     6061 gtgcttgatc agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc
     6121 ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc
     6181 agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc
     6241 agcagggagg gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat
     6301 gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt
     6361 ggccattgct actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc
     6421 tggttcccag cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag
     6481 ctccttaggg cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt
     6541 aatggcagca ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac
     6601 cggcgagtac tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg
     6661 cccggcgtct atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat
     6721 cgggaatcgt tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc
     6781 gatatagccc actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc
     6841 ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa
     6901 atgttggatg ctcatactcg tcctttttca atattattga agcatttatc agggttacta
     6961 gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca
     7021 ataaaatatc
//
