LOCUS       sequence-183259-        5136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  It contains ~0.8 Kb of the miR-183-96-182 cluster promoter. Is the 
            minimal region that contains the maximal promoter activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Segura MF, Jubierre L, Li S, Soriano A, Koetz L, Gaziel-Sovran A, 
            Masanas M, Kleffman K, Dankert JF, Walsh MJ, Hernando E
  TITLE     Kruppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster 
            under physiologic and pathologic conditions.
  JOURNAL   Oncotarget. 2017 Apr 18;8(16):26298-26311. doi: 
            10.18632/oncotarget.15459.
  PUBMED    28412746
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget"; journalName: "Oncotarget"; date: "2017-04-18-
            18"; volume: "8"; issue: "16"; pages: "26298-26311"
COMMENT     Sequence Label: pGL4-Cluster Promoter Delta 1.1
FEATURES             Location/Qualifiers
     source          1..5136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..34
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        996..1027
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1054..1063
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1060..2712
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2756..2877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2767..2786)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2821..2840
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3125..3142)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3296..3884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3376..3395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4084..4875,4876..4944))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5049..5097
                     /note="synthetic polyadenylation signal"
     misc_feature    join(5111..5136,1..66)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
       61 ttctctggcc taactggccg gtaccgagct cggatccact agtaacggcc gccagtgtgc
      121 tgggcagtgg ggggaggggg agatgggaac gggtctgggg ggcggctccc ctccccctcc
      181 ctctcttggg acagacagcc tttgttcccg gcggcggcgg cagcggtggc cagacacctg
      241 tgaggccgca gaactggccg agggtgggcg tgtgaggaca tggaggcccc ttgtccccat
      301 tccagccctg cgcccctggg tccgggtgcg ccccctgaga caggtcagcc ttgagcacct
      361 ttctgcgggg agactgcaga gatcgtccag tggccctacg gtgcgcccca gctcccaacg
      421 cacttcccct cccccctccc agggtcggat ttccaagcac gccacgccac acacacctcg
      481 gctagctccg ggctcagagg gcgctggggt ggcagggcgt ccgggcgcac ctcggctagc
      541 ggcaccctag cccgagggca ggaggcgggg atagaaggcg ggcgacggga ccccttgggg
      601 ccagacccgg ccgggttggg caagcaggcg gggctcccgg cgtctccgca gtcccggacg
      661 ggcgtgccct gcgccgcgca ccaaccggag ccacattcaa ggtgaatcat ccccggcccg
      721 ccccctgccc ctccccgcct cgcggcacct gagccaatgg cgctgcgcga ggggcgggcg
      781 gggcccgggg cggggggcgg taaaaccctc ctggagtcat tctctgtgca ctccgtcgag
      841 cccccgcgca gccgtgccgg agccgcaggg cctccgtcca gccgcgcggt ccgggcagcc
      901 cgcgccccct gttcttccgg tcctcgggcc caggtacctg agctcgctag cctcgaggat
      961 atcaagatct ggcctcggcg gccaagctta gacactagag ggtatataat ggaagctcga
     1021 cttccagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
     1081 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
     1141 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
     1201 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
     1261 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
     1321 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
     1381 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
     1441 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
     1501 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
     1561 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
     1621 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
     1681 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
     1741 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
     1801 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
     1861 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
     1921 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1981 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     2041 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     2101 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     2161 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     2221 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     2281 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     2341 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     2401 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     2461 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     2521 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     2581 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     2641 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     2701 atcgccgtgt aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     2761 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2821 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2881 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2941 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
     3001 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     3061 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     3121 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3181 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3241 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3301 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3361 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3421 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3481 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3541 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3601 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3661 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3721 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3781 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3841 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3901 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3961 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4021 tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag
     4081 tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc
     4141 catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg
     4201 ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat
     4261 gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat
     4321 ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg
     4381 aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc
     4441 gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa
     4501 tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc
     4561 gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt
     4621 ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag
     4681 ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt
     4741 gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag
     4801 atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac
     4861 cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc
     4921 gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca
     4981 gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga
     5041 caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     5101 cgatagtact aacatacgct ctccatcaaa acaaaa
//
