LOCUS       sequence-183261-        7414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7414)
  TITLE     Deisseroth AAV plasmids
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)
FEATURES             Location/Qualifiers
     source          1..7414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     promoter        231..1409
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          462..1400
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1357..1377
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(1440..1473)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1524..1557)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1569..2276)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1847..1866)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2116..2134
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    3388..3421
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3472..3505
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3530..4118
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3583..3603)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4001..4012)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4150..4626
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4349..4368)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4666..4806
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4666..4795
                     /label=AAV2 ITR
     rep_origin      4881..5336
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4968..4987)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5178..5199
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5353..5372)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5472..5494
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5532..5550)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5618..5722
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5723..5791,5792..6583)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5941..5960)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6754..7342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7243..7262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(7404..7414,1..130)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga
      181 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      241 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      301 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      361 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      421 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      481 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      541 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      601 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      661 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      721 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      781 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      841 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      901 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      961 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1021 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1081 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1141 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1201 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1261 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1321 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1381 gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc gactccggaa
     1441 taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga ttgtagctgc
     1501 tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg aagttatggc
     1561 gcgccctatt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
     1621 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
     1681 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
     1741 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
     1801 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
     1861 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
     1921 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
     1981 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
     2041 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
     2101 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
     2161 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
     2221 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccacg
     2281 ttgatgtcga tctggtccag ggggatgtac tcgccctcgc tggtgatcct gctcttggcg
     2341 gccgcgtcct cctcttcagc caccagtgtc tcaacctcca tctcttggcc ggcgatcgta
     2401 attttctgct tctttctgat gtcgccatac aacaggatgt gctcgtggat tttgacgcgc
     2461 aggtaattcc ccagcacacc ccacatgttc tttgcaatca ggtcgaggat ggagtgccca
     2521 atggcagatc cataagggct gatatgaccg aatccttccg tgcccagcag aaacaggact
     2581 ggaaacattc cccaagccac aaagaatgtc caggccatca ctcggaccag ttctcgacaa
     2641 atcccttttg gaactgtgtg gaatgcttcg atataaactt tagcagcatg aaaataggta
     2701 tacataccat agctcaatga gatgaggaag aacaggatct tcgtccaccc cgtacacatg
     2761 gcgctggttg cgccccacac gatgcatccc acatcagaaa ccaacaggcc catagtcctt
     2821 ttgctataat cgtccttcag gcctgtgagg ttgctcagat ggatcagaag gaccgggcag
     2881 gtgagcagcc aggtcgcgta acgaagccac acggtcttgt tgccgttgga tgagtagatc
     2941 accgcaggtt cgtcaaactc atggaaatac tcgatgatga acttgatcat ctcgatcgtg
     3001 gccacgtaaa tggtctccca gccgcaagta gacttccagg tctggtagcc gtagaacatc
     3061 aggcagagcg ctgaaagcgc aaaagtaatc cactgcagaa tgttggcagc caacttctcg
     3121 gcatttgttc cgttggattt aagccaagcc aagcagaagc actggccgtt gttcgggatg
     3181 caaataacag aaccattgtt ctcaagagtg tatgaggttt ggaagagcat gcgctcgtgg
     3241 gcacggtgga aaacgtagtc ggggccatcc tgagtcgcga ccggcaccgt cgcgtcactg
     3301 ccagtcgagg ctcctgcgct gccggccgcc agcgccactg ccagcgctag ggcaagaagc
     3361 catggcctcc gcgacatggt ggctagcata acttcgtata aagtatccta tacgaagtta
     3421 tttgccttaa cccagaaatt atcactgtta ttctttagaa tggtgcaaag aataacttcg
     3481 tataatgtat gctatacgaa gttatgaatt cgatatcaag cttatcgata atcaacctct
     3541 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3601 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3661 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3721 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3781 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3841 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3901 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc
     3961 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4021 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4081 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga
     4141 gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     4201 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     4261 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     4321 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     4381 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     4441 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     4501 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     4561 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     4621 gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga
     4681 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     4741 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     4801 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4861 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4921 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4981 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5041 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     5101 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5161 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5221 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5281 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     5341 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     5401 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     5461 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     5521 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5581 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5641 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5701 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5761 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5821 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5881 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5941 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6001 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6061 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6121 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6181 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6241 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6301 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6361 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6421 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6481 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6541 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6601 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6661 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6721 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6781 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6841 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6901 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6961 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7021 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7081 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7141 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7201 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7261 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7321 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7381 ttgctggcct tttgctcaca tgtcctgcag gcag
//
