LOCUS       sequence-183289-        9070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  over-expression of VEGF-A(165) in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9070)
  TITLE     Fernando Fierro lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE
FEATURES             Location/Qualifiers
     source          1..9070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..57
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(72..77,78..95,96..102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    110..126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     115..137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     134..150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     134..150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     169..189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        171..189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        290..669
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        670..873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     824..844
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             888..1068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1115..1240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1733..1966
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2151..2195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2492..2609
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3837..4336
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3872..3895)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4269..4288
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4357..4366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4363..4365,4366..4368,4369..5082)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4363..4365,4366..4368,4369..5079)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4408..4429)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4669..4688)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5016..5037
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5125..5713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5178..5198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5596..5607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5805..6038
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6110..6231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6147..6166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6201..6220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6271..6406
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6426..6445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6427..6445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6455..6472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6455..6471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6464..6486)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6613..7068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6700..6719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6910..6931
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7094..7198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7199..7267,7268..8059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7417..7436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8230..8818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8719..8738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8972..8989
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
       61 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      121 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
      181 cactaaaggg aacaaaagct ggagctgcaa gcttggccat tgcatacgtt gtatccatat
      241 cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta
      301 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      361 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      421 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      481 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      541 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      601 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      661 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      721 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      781 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      841 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgggg tctctctggt
      901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     1081 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     1141 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1201 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1261 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1321 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1381 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1441 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1501 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1561 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1621 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1681 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1741 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1801 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1861 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1921 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1981 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     2041 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     2101 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2161 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2221 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2281 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2341 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2401 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag
     2461 ctaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2521 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2581 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2641 tttgggaatt agcttgatcg attagtccaa tttgttaaag acaggatatc agtggtccag
     2701 gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata
     2761 gaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     2821 tttggcaagc taggatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga
     2881 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagttgga acagcagaat
     2941 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     3001 atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag
     3061 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     3121 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     3181 ctcggcgcga tctagatctc gaatcgaatt cgcccttcct gagatcaccg gtaggagggc
     3241 catcatgaac tttctgctgt cttgggtgca ttggagcctt gccttgctgc tctacctcca
     3301 ccatgccaag tggtcccagg ctgcacccat ggcagaagga ggagggcaga atcatcacga
     3361 agtggtgaag ttcatggatg tctatcagcg cagctactgc catccaatcg agaccctggt
     3421 ggacatcttc caggagtacc ctgatgagat cgagtacatc ttcaagccat cctgtgtgcc
     3481 cctgatgcga tgcgggggct gctgcaatga cgagggcctg gagtgtgtgc ccactgagga
     3541 gtccaacatc accatgcaga ttatgcggat caaacctcac caaggccagc acataggaga
     3601 gatgagcttc ctacagcaca acaaatgtga atgcagacca aagaaagata gagcaagaca
     3661 agaaaatccc tgtgggcctt gctcagagcg gagaaagcat ttgtttgtac aagatccgca
     3721 gacgtgtaaa tgttcctgca aaaacacaga ctcgcgttgc aaggcgaggc agcttgagtt
     3781 aaacgaacgt acttgcagat gtgacgagcc gaggcggtga aagggcgaat tctaccgggt
     3841 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     3901 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     3961 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     4021 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     4081 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     4141 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     4201 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     4261 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     4321 atctccgggc ctttcgacca tctagatcca ccggtcgcca ccatggtgag caagggcgag
     4381 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4441 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     4501 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     4561 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     4621 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     4681 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     4741 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     4801 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     4861 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     4921 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     4981 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5041 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggcca actcgaggga
     5101 tcccccgggg tcgactgatc cgataatcaa cctctggatt acaaaatttg tgaaagattg
     5161 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5221 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5281 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5341 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5401 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5461 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5521 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5581 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5641 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5701 gccgcctccc cgcatcggat caaattcgag ctcggtacct ttaagaccaa tgacttacaa
     5761 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5821 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5881 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5941 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6001 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6061 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     6121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6241 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6301 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6361 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     6421 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     6481 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6541 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6601 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6661 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6721 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6781 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6841 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6901 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6961 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7021 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     7081 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7141 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7201 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7261 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7321 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7381 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7441 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7501 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7561 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7621 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7681 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7741 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7801 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7861 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7921 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7981 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8041 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8101 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8161 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8221 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8281 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8341 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8401 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8461 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8521 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8581 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8641 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8701 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8761 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8821 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8881 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8941 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     9001 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     9061 cgacaggttt
//
