LOCUS       sequence-183306-        8286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Silencing Hmga2 in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Kalomoiris S, Cicchetto AC, Lakatos K, Nolta JA, Fierro FA
  TITLE     Fibroblast Growth Factor 2 Regulates High Mobility Group A2 
            Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells.
  JOURNAL   J Cell Biochem. 2016 Sep;117(9):2128-37. doi: 10.1002/jcb.25519. 
            Epub 2016 Mar 8.
  PUBMED    26888666
REFERENCE   2  (bases 1 to 8286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jcb.25519";
            journalName: "J Cell Biochem"; date: "2016-09"; volume: "117"; 
            issue: "9"; pages: "2128-37"
COMMENT     Sequence Label: pCCLc-U6-shHMGA2.3-PGK-dTomato
FEATURES             Location/Qualifiers
     source          1..8286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    104..125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(140..145,146..163,164..170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    178..194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     183..205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     202..218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     202..218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     237..257
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        239..257
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        358..737
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        738..941
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     892..912
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             956..1136
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1183..1308
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1801..2034
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2219..2263
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2560..2677
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2850..3098)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2908..2927)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3078..3098)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3156..3655
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3191..3214)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3588..3607
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3676..3685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3682..4383
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3815..3833)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4083..4102
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4277..4300
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4409..4997
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4462..4482)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4880..4891)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5089..5322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5394..5515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5431..5450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5485..5504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5555..5690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5710..5729)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5711..5729)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5739..5756)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5739..5755)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5748..5770)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5897..6352
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5984..6003)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6194..6215
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6378..6482
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6483..6551,6552..7343)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6701..6720)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7514..8102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8003..8022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8256..8273
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
       61 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      121 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      181 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
      241 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg catacgttgt
      301 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
      361 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      421 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      481 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      541 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      601 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      661 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      721 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      781 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      841 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      901 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccggggtc
      961 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     1021 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1081 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     1141 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     1201 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1261 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1321 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1381 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1441 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1501 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1561 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1621 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     1681 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1741 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1801 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1861 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1921 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1981 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     2041 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     2101 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2161 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2221 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2281 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2341 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2401 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2461 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     2521 tcgataagct aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2581 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2641 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2701 agatccagtt tgggaattag cttgatcgat tagtccaatt tgttaaagac aggatgatcc
     2761 ccgggtaccg agctcgaatt cgcccttaaa aaagggaaga ggaggaggaa ttttccagtg
     2821 ttgagaggaa aattcctcct cctcttcccg gtgtttcgtc ctttccacaa gatatataaa
     2881 gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat
     2941 aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact
     3001 aatatctttg tgtttacagt caaattaatt ctaattatct ctctaacagc cttgtatcgt
     3061 atatgcaaat atgaaggaat catgggaaat aggccctctt cctgcccgac cttggatccc
     3121 caaactggat ctgttaacca agggcgaatt ctaccgggta ggggaggcgc ttttcccaag
     3181 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     3241 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     3301 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     3361 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     3421 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     3481 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     3541 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     3601 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgaccat
     3661 ctagatccac cggtcgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg
     3721 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     3781 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     3841 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     3901 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     3961 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     4021 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     4081 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     4141 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     4201 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     4261 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     4321 cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac
     4381 aagtaggcgg ggggtcgact gatccgataa tcaacctctg gattacaaaa tttgtgaaag
     4441 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4501 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4561 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4621 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4681 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4741 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4801 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4861 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4921 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4981 ttgggccgcc tccccgcatc ggatcaaatt cgagctcggt acctttaaga ccaatgactt
     5041 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5101 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5161 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5221 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5281 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     5341 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5401 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5461 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5521 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5581 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5641 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     5701 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     5761 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5821 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5881 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5941 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6001 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6061 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6121 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6181 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6241 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6301 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6361 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6421 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6481 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6541 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6601 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6661 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6721 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6781 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6841 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6901 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6961 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7021 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7081 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7141 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7201 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7261 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7321 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7381 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7441 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7501 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7561 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7621 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7681 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7741 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7801 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7861 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7921 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7981 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8041 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8101 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8161 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8221 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8281 aagagc
//
