LOCUS       sequence-183316-       10144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Luciferase in human cells. 3rd generation lentiviral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Beegle J, Lakatos K, Kalomoiris S, Stewart H, Isseroff RR, Nolta JA,
            Fierro FA
  TITLE     Hypoxic preconditioning of mesenchymal stromal cells induces 
            metabolic changes, enhances survival, and promotes cell retention in
            vivo.
  JOURNAL   Stem Cells. 2015 Jun;33(6):1818-28. doi: 10.1002/stem.1976. Epub 
            2015 May 11.
  PUBMED    25702874
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/stem.1976";
            journalName: "Stem Cells"; date: "2015-06"; volume: "33"; issue: 
            "6"; pages: "1818-28"
COMMENT     Sequence Label: pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE
FEATURES             Location/Qualifiers
     source          1..10144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    117..138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(153..158,159..176,177..183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    191..207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     196..218
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     215..231
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     215..231
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     250..270
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        252..270
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        371..750
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        751..954
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     905..925
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             969..1149
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1196..1321
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1814..2047
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2232..2276
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2573..2690
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3326..4978
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3392..3410)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        4996..5495
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5031..5054)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5428..5447
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      5516..5525
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5522..5524,5525..5527,5528..6241)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5522..5524,5525..5527,5528..6238)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5567..5588)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5828..5847)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6175..6196
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6270..6858
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6323..6343)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6741..6752)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6960..7193
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7265..7386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7302..7321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7356..7375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7426..7561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7581..7600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7582..7600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7610..7627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7610..7626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7619..7641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7768..8223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7855..7874)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8065..8086
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8249..8353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8354..8422,8423..9214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8572..8591)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9385..9973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9874..9893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10127..10144
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
       61 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      121 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      181 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
      241 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttggcca
      301 ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta
      361 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      421 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      481 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      541 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      601 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      661 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      721 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      781 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      841 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      901 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      961 gtgaaccggg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     1021 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     1081 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     1141 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1201 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1261 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1321 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1381 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1441 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1501 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1561 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1621 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1681 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1741 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1801 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1861 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1921 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1981 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     2041 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     2101 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     2161 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2221 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2281 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2341 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2401 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2461 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2521 gatctcgacg gtatcgataa gctaattcac aaatggcagt attcatccac aattttaaaa
     2581 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2641 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2701 acagggacag cagagatcca gtttgggaat tagcttgatc gattagtcca atttgttaaa
     2761 gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct
     2821 atagagtacg agccatagat agaataaaag attttattta gtctccagaa aaagggggga
     2881 atgaaagacc ccacctgtag gtttggcaag ctaggatcaa ggttaggaac agagagacag
     2941 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     3001 gaacagttgg aacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     3061 ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag
     3121 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     3181 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     3241 aaaagagccc acaacccctc actcggcgcg atctagatct cgaatcgaat tcgttaacct
     3301 cgagggatct cctaggaagc tttccatgga agacgccaaa aacataaaga aaggcccggc
     3361 gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag
     3421 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac
     3481 ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     3541 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     3601 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     3661 tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg
     3721 gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat
     3781 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     3841 acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat
     3901 tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca
     3961 tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     4021 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     4081 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     4141 gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt
     4201 ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     4261 tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca
     4321 tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac
     4381 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     4441 ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt
     4501 gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     4561 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     4621 cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc
     4681 tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct
     4741 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     4801 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     4861 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     4921 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt
     4981 ctagagaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     5041 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     5101 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     5161 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     5221 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     5281 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     5341 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     5401 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     5461 cgctgttctc ctcttcctca tctccgggcc tttcgaccat ctagatccac cggtcgccac
     5521 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5581 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5641 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5701 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     5761 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5821 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5881 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5941 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     6001 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     6061 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     6121 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     6181 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     6241 aagcggccaa ctcgagggat ccccccgata atcaacctct ggattacaaa atttgtgaaa
     6301 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6361 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6421 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6481 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6541 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6601 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6661 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6721 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6781 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6841 tttgggccgc ctccccgcat cgggggtcga ctgatcaaat tcgagctcgg tacctttaag
     6901 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6961 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7021 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7081 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7141 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7201 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7261 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7321 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7381 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     7441 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7501 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7561 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     7621 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7681 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7741 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     7801 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7861 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7921 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7981 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8041 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8101 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8161 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     8221 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8281 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8341 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8401 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8461 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8521 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8581 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8641 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8701 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8761 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8821 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8881 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8941 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9001 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9061 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9121 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9181 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9241 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9301 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9361 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9421 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9481 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9541 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9601 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9661 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9721 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9781 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9841 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9901 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9961 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10021 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10081 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    10141 cgag
//
