LOCUS       sequence-183317-        6229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MMTV-LTR promoter/enhancer with rabbit beta-globin intron and bovine
            growth hormone poly-A sequence to enhance transgene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Tepera SB, McCrea PD, Rosen JM
  TITLE     A beta-catenin survival signal is required for normal lobular 
            development in the mammary gland.
  JOURNAL   J Cell Sci. 2003 Mar 15;116(Pt 6):1137-49.
  PUBMED    12584256
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2003-03-15"; volume: "116(Pt 6)"; pages: "1137-49"
COMMENT     Sequence Label: MKbpAII
FEATURES             Location/Qualifiers
     source          1..6229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..21
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3..21
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     58..74
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          2431..3003
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3011..3030
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3079..3096)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3085..3309
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3376..3392)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3377..3393)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3417..3436)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3418..3436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3446..3463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3446..3462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3455..3477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3604..4059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3691..3710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3901..3922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4085..4189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4190..4258,4259..5050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4408..4427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5221..5809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5710..5729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5963..5980
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6097..6118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6133..6138,6139..6156,6157..6163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6171..6187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6176..6198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6195..6211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6195..6211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgctct
       61 agaactagtg gatcccaatg atagagattt tactgctcta gttccccata cagaattgtt
      121 tcgcttagtt gcagcctcaa gatatcttat tctcaaaagg ccaggatttc aagaacatga
      181 catgattcct acatctgcct gtgttactta cccttatgcc atattattag gattacctca
      241 gctaatagat atagagaaaa gaggatctac ttttcatatt tcctgttctt cttgtagatt
      301 gactaattgt ttagattctt ctgcctacga ctatgcagcg atcatagtca agaggccgcc
      361 atacgtgctg ctacctgtag atattggtga tgaaccatgg tttgatgatt ctgccattca
      421 aacctttagg tatgccacag atttaattcg agccaagcga ttcgtcgctg ccattattct
      481 gggcatatct gctttaattg ctattatcac ttcctttgct gtagctacta ctgctttagt
      541 taaggagatg caaactgcta cgtttgttaa taatcttcat aggaatgtta cattagcctt
      601 atctgaacaa agaataatag atttaaaatt agaagctaga cttaatgctt tagaagaagt
      661 agttttagag ttgggacaag atgtggcaaa cttaaagacc agaatgtcca ccaggtgtca
      721 tgcaaattat gattttatct gcgttacacc tttaccatat aatgcttctg agagctggga
      781 aagaaccaaa gctcatttat tgggcatttg gaatgacaat gagatttcat ataacataca
      841 agaattaacc aacctgatta gtgatatgag caaacaacat attgacacag tggacctcag
      901 tggcttggct cagtcctttg ccaatggagt aaaggcttta aatccattag attggacaca
      961 atatttcatt tttataggtg ttggagccct gcttttagtc atagtgctta tgattttccc
     1021 cattgttttc cagtgccttg cgaagagcct tgaccaagtg cagtcagatc ttaacgtgct
     1081 tcttttaaaa aagaaaaaag ggggaaatgc cgcgcctgca gcagaaatgg ttgaactccc
     1141 gagagtgtcc tacacctagg ggagaagcag ccaaggggtt gtttcccacc aaggacgacc
     1201 cgtctgcgca caaacggatg agcccatcag acaaagacat attcattctc tgctgcaaac
     1261 ttggcatagc tctgctttgc ctggggctat tgggggaagt tgcggttcgt gctcgcaggg
     1321 ctctcaccct tgactctttt aatagctctt ctgtgcaaga ttacaatcta aacaattcgg
     1381 agaactcgac cttcctcctg aggcaaggac cacagccaac ttcctcttac aagccgcatc
     1441 gattttgtcc ttcagaaata gaaataagaa tgcttgctaa aaattatatt tttaccaata
     1501 agaccaatcc aataggtaga ttattagtta ctatgttaag aaatgaatca ttatctttta
     1561 gtactatttt tactcaaatt cagaagttag aaatgggaat agaaaataga aagagacgct
     1621 caacctcaat tgaagaacag gtgcaaggac tattgaccac aggcctagaa gtaaaaaagg
     1681 gaaaaaagag tgtttttgtc aaaataggag acaggtggtg gcaaccaggg acttataggg
     1741 gaccttacat ctacagacca acagatgccc ccttaccata tacaggaaga tatgacttaa
     1801 attgggatag gtgggttaca gtcaatggct ataaagtgtt atatagatcc ctcccttttc
     1861 gtgaaagact cgccagagct agacctcctt ggtgtatgtt gtctcaagaa gaaaaagacg
     1921 acatgaaaca acaggtacat gattatattt atctaggaac aggaatgcac ttttggggaa
     1981 agattttcca taccaaggag gggacagtgg ctggactaat agaacattat tctgcaaaaa
     2041 cttatggcat gagttattat gaatagcctt tattggccca accttgcggt tcccagggct
     2101 taagtaagtt tttggttaca aactgttctt aaaacgagga tgtgagacaa gtggtttcct
     2161 gacttggttt ggtatcaaag gttctgatct gagctctgag tgttctattt tcctatgttc
     2221 ttttggaatt tatccaaatc ttatgtaaat gcttatgtaa accaagatat aaaagagtgc
     2281 tgattttttg agtaaacttg caacagtcct aacattcacc tcttgtgtgt ttgtgtctgt
     2341 tcgccatccc gtctccgctc gtcacttatc cttcactttc cagagggtcc ccccgcagac
     2401 cccggcgtag aggatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc
     2461 tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt
     2521 agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac
     2581 tttctactct gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt
     2641 tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca
     2701 gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat attatattgt
     2761 acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg
     2821 catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc
     2881 atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa
     2941 tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta
     3001 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcgat
     3061 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3241 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3301 gctctatggc ttctgaggcg gaaagaacca gctggggctc gagatccact agttctagcc
     3361 tcgaatcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccc aattcgccct
     3421 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3481 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3541 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3601 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3661 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3721 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3781 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3841 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3901 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3961 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4021 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     4081 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4141 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4201 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4261 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4321 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4381 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4441 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4501 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4561 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4621 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4681 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4741 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4801 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4861 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4921 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4981 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5041 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5101 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5161 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5221 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5281 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5341 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5401 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5461 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5521 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5581 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5641 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5701 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5761 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5821 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5881 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5941 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     6001 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     6061 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     6121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     6181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgc
//
