LOCUS       sequence-183321-        9126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of LIN28A in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Kalomoiris S, Cicchetto AC, Lakatos K, Nolta JA, Fierro FA
  TITLE     Fibroblast Growth Factor 2 Regulates High Mobility Group A2 
            Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells.
  JOURNAL   J Cell Biochem. 2016 Sep;117(9):2128-37. doi: 10.1002/jcb.25519. 
            Epub 2016 Mar 8.
  PUBMED    26888666
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jcb.25519";
            journalName: "J Cell Biochem"; date: "2016-09"; volume: "117"; 
            issue: "9"; pages: "2128-37"
COMMENT     Sequence Label: pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE
FEATURES             Location/Qualifiers
     source          1..9126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    131..152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(167..172,173..190,191..197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     210..232
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     229..245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     229..245
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     264..284
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        266..284
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        385..764
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        765..968
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     919..939
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             983..1163
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1210..1335
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1828..2061
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2246..2290
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2587..2704
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3988..4487
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4023..4046)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4420..4439
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4508..4517
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4514..4516,4517..4519,4520..5233)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4514..4516,4517..4519,4520..5230)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4559..4580)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4820..4839)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5167..5188
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5276..5864
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5329..5349)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5747..5758)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5956..6189
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6261..6382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6298..6317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6352..6371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6422..6557
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6577..6596)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6578..6596)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6606..6623)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6606..6622)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6615..6637)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6764..7219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6851..6870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7061..7082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7245..7349
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7350..7418,7419..8210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7568..7587)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8381..8969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8870..8889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(9123..9126,1..14)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
       61 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      121 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      181 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      241 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
      301 tgcaagcttg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
      361 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
      421 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      481 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      541 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      601 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      661 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      721 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      781 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      841 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      901 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      961 gcagagctcg tttagtgaac cggggtctct ctggttagac cagatctgag cctgggagct
     1021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     1081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1141 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     1201 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     1261 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1321 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1381 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1441 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1501 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1561 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1621 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1681 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1741 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1801 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1861 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1921 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1981 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     2041 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     2101 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     2161 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     2221 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     2281 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2341 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2401 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2461 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2521 tcgattagtg aacggatctc gacggtatcg ataagctaat tcacaaatgg cagtattcat
     2581 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2641 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2701 ttttcgggtt tattacaggg acagcagaga tccagtttgg gaattagctt gatcgattag
     2761 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
     2821 accagctgaa gcctatagag tacgagccat agatagaata aaagatttta tttagtctcc
     2881 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagga tcaaggttag
     2941 gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     3001 cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt
     3061 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc
     3121 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     3181 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     3241 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgatctag atctcgaatc
     3301 gaattaattc gattatcgcc atgggctccg tgtccaacca gcagtttgca ggtggctgcg
     3361 ccaaggcggc agaagaggcg cccgaggagg cgccggagga cgcggcccgg gcggcggacg
     3421 agcctcagct gctgcacggt gcgggcatct gtaagtggtt caacgtgcgc atggggttcg
     3481 gcttcctgtc catgaccgcc cgcgccgggg tcgcgctcga ccccccagtg gatgtctttg
     3541 tgcaccagag taagctgcac atggaagggt tccggagctt gaaggagggt gaggcagtgg
     3601 agttcacctt taagaagtca gccaagggtc tggaatccat ccgtgtcacc ggacctggtg
     3661 gagtattctg tattgggagt gagaggcggc caaaaggaaa gagcatgcag aagcgcagat
     3721 caaaaggaga caggtgctac aactgtggag gtctagatca tcatgccaag gaatgcaagc
     3781 tgccacccca gcccaagaag tgccacttct gccagagcat cagccatatg gtagcctcat
     3841 gtccgctgaa ggcccagcag ggccctagtg cacagggaaa gccaacctac tttcgagagg
     3901 aagaagaaga aatccacagc cctaccctgc tcccggaggc acagaattga gtttaaacaa
     3961 tcactagttc gaaggatcaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     4021 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     4081 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     4141 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     4201 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     4261 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     4321 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     4381 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     4441 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc atctagatcc
     4501 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4561 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4621 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4681 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4741 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4801 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4861 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4921 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4981 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     5041 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5101 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     5161 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5221 gctgtacaag taaagcggcc aactcgaggg atcccccggg gtcgactgat ccgataatca
     5281 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5341 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5401 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5461 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5521 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5581 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5641 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5701 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5761 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5821 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgga tcaaattcga
     5881 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5941 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     6001 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6061 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6121 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6181 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6241 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6301 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6361 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     6421 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6481 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6541 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6601 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6661 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6721 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6841 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6901 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6961 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7021 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7081 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7141 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7201 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8521 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8761 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9121 gcagcg
//
