LOCUS       sequence-18334-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Bennett MJ, Huey-Tubman KE, Herr AB, West AP, Ross SA, Bjorkman PJ
  TITLE     Inaugural Article: A linear lattice model for polyglutamine in 
            CAG-expansion diseases.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9.
  PUBMED    12193654
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Sep 3. 99(18):11634-9."
COMMENT     Sequence Label: pET32a-HD46Q
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..354
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEI
                     ADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
                     ANLA"
     CDS             406..420
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             762..779
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttaacttta agaaggagat atacatatgt agcgataaaa ttattcacct gactgacgac
       61 agttttgaca cggatgtact caaagcggac ggggcgatcc tcgtcgattt ctgggcagag
      121 tggtgcggtc cgtgcaaaat gatcgccccg attctggatg aaatcgctga cgaatatcag
      181 ggcaaactga ccgttgcaaa actgaacatc gatcaaaacc ctggcactgc gccgaaatat
      241 ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa
      301 gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg acgctaacct ggccggttct
      361 ggttctggcg aacgccagca catggacagc ccagatctgg gtaccgacga cgacgacaag
      421 gccatggcga ccctggaaaa gctgatgaag gccttcgagt ccctcaaaag cttccaacag
      481 cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca acagcagcaa
      541 cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag
      601 caacagcaac aaccgccacc acctccccct ccacccccac ctcctcaact tcctcaacct
      661 cctccacagg cacagcctct gctgcctcag ccacaacctc ctccacctcc acctcacctc
      721 ctccaggccc agctgtggct gaggagcctc tgcaccgacc tcatcatcat catcatcatt
      781 agga
//
