LOCUS       sequence-18335-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Bennett MJ, Huey-Tubman KE, Herr AB, West AP, Ross SA, Bjorkman PJ
  TITLE     Inaugural Article: A linear lattice model for polyglutamine in 
            CAG-expansion diseases.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9.
  PUBMED    12193654
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Sep 3. 99(18):11634-9."
COMMENT     Sequence Label: pET32a-TRXtag
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..365
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             417..431
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             432..449
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             499..516
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(569..587)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      583..630
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(754..773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagataatt ttgttaactt taagaaggag atatacatat gagcgataaa attattcacc
       61 tgactgacga cagttttgac acggatgtac tcaaagcgga cggggcgatc ctcgtcgatt
      121 tctgggcaga gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat gaaatcgctg
      181 acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcaaaac cctggcactg
      241 cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac ggtgaagtgg
      301 cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc gacgctaacc
      361 tggccggttc tggttctggc gaacgccagc acatggacag cccagatctg ggtaccgacg
      421 acgacgacaa gcatcatcat catcatcatt gaggatccga attcgagctc cgtcgacaag
      481 cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      541 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      601 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
      661 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      721 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      781 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      841 gatttagtgc tttacggcac ctcg
//
