LOCUS       sequence-183369-       13455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below 
            on P2A cleaving efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13455)
  AUTHORS   Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, 
            Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu 
            TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley 
            GQ
  TITLE     Haematopoietic stem and progenitor cells from human pluripotent stem
            cells.
  JOURNAL   Nature. 2017 May 17. doi: 10.1038/nature22370.
  PUBMED    28514439
REFERENCE   2  (bases 1 to 13455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 May 17. doi: 10.1038/nature22370."
COMMENT     Sequence Label: pINDUCER-21-RUNX1-P2A-ERG
FEATURES             Location/Qualifiers
     source          1..13455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(1..19,20..42,43..61,62..84,85..103,104..126,127..145,
                     146..168,169..187,188..210,211..229,230..252,253..271)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        304..342
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     339..363
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    472..496
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3455..3479)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3532..3558
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3778..4956
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4009..4947
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4904..4924
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5026..5059
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5066..5683,5684..5689,5690..5725,5726..5728,5729..5764,
                     5765..5767,5768..5803,5804..5812)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5865..6438
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6032..6049)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6259..6278
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6454..6456,6457..6459,6460..7173)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6454..6456,6457..6459,6460..7170)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6499..6520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6760..6779)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7107..7128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7309..7328
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7616..8204
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7669..7689)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8087..8098)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8279..8512
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8631..8649)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8717..8821
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8822..8890,8891..9682)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9040..9059)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9853..10441
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10342..10361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10595..10612
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10852..10873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10888..10893,10894..10911,10912..10918)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10926..10942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10931..10953
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10950..10966
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10950..10966
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11016..11035
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11227..11860
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11907..12032
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12529..12762
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12947..12991
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13284..13401
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt
      301 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      361 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      421 agcctccgcg gccccgaact agtccagtgt ggtggaattc tgcagatatc aacaagtttg
      481 tacaaaaaag caggctccgc ggccgccccc ttcaccatgg cttcagacag catatttgag
      541 tcatttcctt cgtacccaca gtgcttcatg agagaatgca tacttggaat gaatccttct
      601 agagacgtcc acgatgccag cacgagccgc cgcttcacgc cgccttccac cgcgctgagc
      661 ccaggcaaga tgagcgaggc gttgccgctg ggcgccccgg acgccggcgc tgccctggcc
      721 ggcaagctga ggagcggcga ccgcagcatg gtggaggtgc tggccgacca cccgggcgag
      781 ctggtgcgca ccgacagccc caacttcctc tgctccgtgc tgcctacgca ctggcgctgc
      841 aacaagaccc tgcccatcgc tttcaaggtg gtggccctag gggatgttcc agatggcact
      901 ctggtcactg tgatggctgg caatgatgaa aactactcgg ctgagctgag aaatgctacc
      961 gcagccatga agaaccaggt tgcaagattt aatgacctca ggtttgtcgg tcgaagtgga
     1021 agagggaaaa gcttcactct gaccatcact gtcttcacaa acccaccgca agtcgccacc
     1081 taccacagag ccatcaaaat cacagtggat gggccccgag aacctcgaag acatcggcag
     1141 aaactagatg atcagaccaa gcccgggagc ttgtcctttt ccgagcggct cagtgaactg
     1201 gagcagctgc ggcgcacagc catgagggtc agcccacacc acccagcccc cacgcccaac
     1261 cctcgtgcct ccctgaacca ctccactgcc tttaaccctc agcctcagag tcagatgcag
     1321 gatacaaggc agatccaacc atccccaccg tggtcctacg atcagtccta ccaatacctg
     1381 ggatccattg cctctccttc tgtgcaccca gcaacgccca tttcacctgg acgtgccagc
     1441 ggcatgacaa ccctctctgc agaactttcc agtcgactct caacggcacc cgacctgaca
     1501 gcgttcagcg acccgcgcca gttccccgcg ctgccctcca tctccgaccc ccgcatgcac
     1561 tatccaggcg ccttcaccta ctccccgacg ccggtcacct cgggcatcgg catcggcatg
     1621 tcggccatgg gctcggccac gcgctaccac acctacctgc cgccgcccta ccccggctcg
     1681 tcgcaagcgc agggaggccc gttccaagcc agctcgccct cctaccacct gtactacggc
     1741 gcctcggccg gctcctacca gttctccatg gtgggcggcg agcgctcgcc gccgcgcatc
     1801 ctgccgccct gcaccaacgc ctccaccggc tccgcgctgc tcaaccccag cctcccgaac
     1861 cagagcgacg tggtggaggc cgagggcagc cacagcaact cccccaccaa catggcgccc
     1921 tccgcgcgcc tggaggaggc cgtgtggagg ccctacttgg ccacgaagca agcaggagat
     1981 gttgaagaaa accccgggcc tatggccagc actattaagg aagccttatc agttgtgagt
     2041 gaggaccagt cgttgtttga gtgtgcctac ggaacgccac acctggctaa gacagagatg
     2101 accgcgtcct cctccagcga ctatggacag acttccaaga tgagcccacg cgtccctcag
     2161 caggattggc tgtctcaacc cccagccagg gtcaccatca aaatggaatg taaccctagc
     2221 caggtgaatg gctcaaggaa ctctcctgat gaatgcagtg tggccaaagg cgggaagatg
     2281 gtgggcagcc cagacaccgt tgggatgaac tacggcagct acatggagga gaagcacatg
     2341 ccacccccaa acatgaccac gaacgagcgc agagttatcg tgccagcaga tcctacgcta
     2401 tggagtacag accatgtgcg gcagtggctg gagtgggcgg tgaaagaata tggccttcca
     2461 gacgtcaaca tcttgttatt ccagaacatc gatgggaagg aactgtgcaa gatgaccaag
     2521 gacgacttcc agaggctcac ccccagctac aatgccgaca tccttctctc acatctccac
     2581 tacctcagag agactcctct tccacatttg acttcagatg atgttgataa agccttacaa
     2641 aactctccac ggttaatgca tgctagaaac acagggggtg cagcttttat tttcccaaat
     2701 acttcagtat atcctgaagc tacgcaaaga attacaacta ggccagattt accatatgag
     2761 ccccccagga gatcagcctg gaccggtcac ggccacccca cgccccagtc gaaagctgct
     2821 caaccatctc cttccacagt gcccaaaact gaagaccagc gtcctcagtt agatccttat
     2881 cagattcttg gaccaacaag tagccgcctt gcaaatccag gcagtggcca gatccagctt
     2941 tggcagttcc tcctggagct cctgtcggac agctccaact ccagctgcat cacctgggaa
     3001 ggcaccaacg gggagttcaa gatgacggat cccgacgagg tggcccggcg ctggggagag
     3061 cggaagagca aacccaacat gaactacgat aagctcagcc gcgccctccg ttactactat
     3121 gacaagaaca tcatgaccaa ggtccatggg aagcgctacg cctacaagtt cgacttccac
     3181 gggatcgccc aggccctcca gccccacccc ccggagtcat ctctgtacaa gtacccctca
     3241 gacctcccgt acatgggctc ctatcacgcc cacccacaga agatgaactt tgtggcgccc
     3301 caccctccag ccctccccgt gacatcttcc agtttttttg ctgccccaaa cccatactgg
     3361 aattcaccaa ctgggggtat ataccccaac actaggctcc ccaccagcca tatgccttct
     3421 catctgggca cttactacaa gggtgggcgc gccgacccag ctttcttgta caaagtggtt
     3481 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga atacccatac
     3541 gacgtcccag actacgctta gtaatgatta attaaactag aaattctacc gggtagggga
     3601 ggcgcttttc ccaaggcagt ctggagtaac ccacccaaga tctggcctcc gcgccgggtt
     3661 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcc gcgtaagatc tggcctccgc
     3721 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagccta gtcgtgaggc
     3781 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     3841 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     3901 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     3961 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     4021 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     4081 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     4141 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     4201 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     4261 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     4321 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     4381 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     4441 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     4501 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     4561 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     4621 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     4681 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     4741 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     4801 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     4861 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     4921 gttcaaagtt tttttcttcc atttcaggtg tcgtgaagcg gccgccatgc tagtaaattg
     4981 tccgctaaat tctggccgtt tttggctttt ttgttagacg gatccataac ttcgtataat
     5041 gtatgctata cgaagttatg ccaccatgtc tagactggac aagagcaaag tcataaacgg
     5101 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
     5161 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     5221 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     5281 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     5341 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     5401 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     5461 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     5521 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     5581 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     5641 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
     5701 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
     5761 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa
     5821 ggatccgcgg ccgcactaga ggaattccgc ccctctcccc cccccccctc tccctccccc
     5881 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     5941 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     6001 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     6061 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     6121 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     6181 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     6241 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     6301 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     6361 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     6421 tgaaaaacac gatgataagc ttaccggtcc accatggtga gcaagggcga ggagctgttc
     6481 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     6541 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     6601 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     6661 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     6721 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     6781 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     6841 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     6901 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     6961 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     7021 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     7081 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     7141 atcactctcg gcatggacga gctgtacaag taagcatgcc cgacggcgag gatctcgtcg
     7201 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7261 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7321 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7381 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgat
     7441 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     7501 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7561 attgttgttg tttagtccct cccaattcga tatcaagctt atcgcgatag atcctaatca
     7621 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7681 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7741 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7801 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7861 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7921 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7981 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     8041 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8101 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     8161 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     8221 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8281 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8341 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8401 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8461 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8521 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8581 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     8641 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     8701 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8761 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8821 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8881 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8941 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     9001 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9061 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9121 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9181 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9241 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9301 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9361 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9421 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9481 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9541 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9601 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9661 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9721 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9781 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9841 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9901 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9961 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    10021 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10081 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10141 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10201 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10261 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10321 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10381 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10441 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    10501 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    10561 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10621 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
    10681 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    10741 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
    10801 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10861 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10921 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
    10981 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11041 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    11101 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    11161 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    11221 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
    11281 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
    11341 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
    11401 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
    11461 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
    11521 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
    11581 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
    11641 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
    11701 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    11761 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    11821 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
    11881 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
    11941 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
    12001 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
    12061 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
    12121 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
    12181 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
    12241 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
    12301 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
    12361 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
    12421 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
    12481 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
    12541 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
    12601 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
    12661 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
    12721 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    12781 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    12841 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    12901 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    12961 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    13021 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    13081 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    13141 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
    13201 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca
    13261 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
    13321 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    13381 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact
    13441 aggatccttt accac
//
