LOCUS       sequence-183375-        8646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Silencing Hmga2 in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Kalomoiris S, Cicchetto AC, Lakatos K, Nolta JA, Fierro FA
  TITLE     Fibroblast Growth Factor 2 Regulates High Mobility Group A2 
            Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells.
  JOURNAL   J Cell Biochem. 2016 Sep;117(9):2128-37. doi: 10.1002/jcb.25519. 
            Epub 2016 Mar 8.
  PUBMED    26888666
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jcb.25519";
            journalName: "J Cell Biochem"; date: "2016-09"; volume: "117"; 
            issue: "9"; pages: "2128-37"
COMMENT     Sequence Label: pCCLc-U6-shHMGA2.2-PGK-dTomato
FEATURES             Location/Qualifiers
     source          1..8646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(268..273,274..291,292..298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     311..333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     365..385
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        367..385
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        486..865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        866..1069
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1020..1040
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1084..1264
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1311..1436
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1929..2162
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2347..2391
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2688..2805
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2973..3221)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(3338..3586)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        3644..4143
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3679..3702)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4076..4095
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4164..4173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4170..4871
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(4303..4321)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4571..4590
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4765..4788
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4897..5485
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4950..4970)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5368..5379)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5577..5810
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5882..6003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5919..5938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5973..5992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6043..6178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6198..6217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6199..6217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6227..6244)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6227..6243)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6236..6258)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6385..6840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6472..6491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6682..6703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6866..6970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6971..7039,7040..7831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7189..7208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8002..8590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8491..8510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt ggccattgca
      421 tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc
      481 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      541 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      601 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      661 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      721 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      781 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      841 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      901 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      961 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1021 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     1081 ccggggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     1141 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     1201 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1261 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1321 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1381 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1441 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1501 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1561 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1621 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1681 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1741 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1801 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1861 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1921 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1981 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     2041 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     2101 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     2161 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     2221 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2281 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2341 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2401 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2461 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2521 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2581 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     2641 cgacggtatc gataagctaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa
     2701 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2761 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     2821 gacagcagag atccagtttg ggaattagct tgatcgatta gtccaatttg ttaaagacag
     2881 gatgatcccc gggtaccgag ctcgaattcg cccttaaaaa agggaagagg aggaggaatt
     2941 tttctcttgg taaaattcct cctcctcttc ccggtgtttc gtcctttcca caagatatat
     3001 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
     3061 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
     3121 actaatatct ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat
     3181 cgtatatgca aatatgaagg aatcatggga aataggccct cttcctgccc gaccttggat
     3241 ccccaaactg gatctgttaa ccaagggcga attcgccctt aaaaaaggga agaggaggag
     3301 gaatttttct cttggtaaaa ttcctcctcc tcttcccggt gtttcgtcct ttccacaaga
     3361 tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt
     3421 taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat
     3481 tttgtactaa tatctttgtg tttacagtca aattaattct aattatctct ctaacagcct
     3541 tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctcttcc tgcccgacct
     3601 tggatcccca aactggatct gttaaccaag ggcgaattct accgggtagg ggaggcgctt
     3661 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     3721 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     3781 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     3841 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     3901 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     3961 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     4021 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     4081 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     4141 tcgaccatct agatccaccg gtcgccacca tggtgagcaa gggcgaggag gtcatcaaag
     4201 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
     4261 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     4321 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
     4381 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
     4441 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
     4501 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
     4561 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     4621 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
     4681 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
     4741 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
     4801 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg
     4861 agctgtacaa gtaggcgggg ggtcgactga tccgataatc aacctctgga ttacaaaatt
     4921 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4981 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5041 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5101 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5161 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5221 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5281 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     5341 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5401 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5461 atctcccttt gggccgcctc cccgcatcgg atcaaattcg agctcggtac ctttaagacc
     5521 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5581 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5641 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5701 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5761 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     5821 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     5881 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5941 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6001 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6061 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6121 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6181 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6241 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6301 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6361 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6421 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6481 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6541 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6601 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6661 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6721 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6781 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6841 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6901 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6961 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7021 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7081 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7141 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7201 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7261 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7321 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7381 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7441 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7501 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7561 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7621 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7681 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7741 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7801 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7861 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7921 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7981 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8041 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8101 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8161 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8221 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8281 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8341 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8401 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8461 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8521 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8581 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8641 tgctca
//
