LOCUS       sequence-183381-        7851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector encoding Cre-dependent expression of 
            EYFP-P2A-Mafb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Mi D, Li Z, Lim L, Li M, Moissidis M, Yang Y, Gao T, Hu TX, Pratt T,
            Price DJ, Sestan N, Marin O
  TITLE     Early emergence of cortical interneuron diversity in the mouse 
            embryo.
  JOURNAL   Science. 2018 Feb 22. pii: science.aar6821. doi: 
            10.1126/science.aar6821.
  PUBMED    29472441
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 22. pii: science.aar6821. doi: 10.1126/science.aar6821."
COMMENT     Sequence Label: RV-Cag-Dio-EYFP-P2A-Mafb
FEATURES             Location/Qualifiers
     source          1..7851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     321..343
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     405..424
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(467..500)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(551..584)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1064..1081)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1151..1174)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(1574..1630)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(1646..2356,2357..2359,2360..2362))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1688..1709)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2037..2056
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2296..2317
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2359..2368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2380..2413
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    2464..2497
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2507..3095
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2560..2580)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2978..2989)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3290..3759
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3889..3906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4060..4648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4140..4159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4808..5599,5600..5668))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5431..5450
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        5820..6199
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6200..6403
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6354..6374
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6404..6579
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    6642..6841
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7042..7458
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7388..7410
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7426..7442
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        7472..7851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
       61 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
      121 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
      181 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
      241 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
      301 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
      361 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
      421 tgtgctgtct catcattttg gcaaagaatt cgctagcgga tccggaataa cttcgtatag
      481 gatactttat acgaagttat gcagaatggt agctggattg tagctgctat tagcaatatg
      541 aaacctctta ataacttcgt atagcataca ttatacgaag ttatttaatt aatcacagaa
      601 agaactcagg agaggagggg ctgtcgctgg tggagcccgc ctccctgaag ccggagttgg
      661 cgagtttctc gcacttgacc ttgtaggcgt ctctctcgcg ggccagccgg gacacctcct
      721 gcttaagctg ctccacctgc tgaatgagct gcgtcttctc gttctccagg tgatgtttct
      781 gctggacgcg tttatacctg cacgactggg cgtagccccg gttcttcagg gtccgccgct
      841 tctgcttcag gcggatcacc tcgtccttgg tgaagccccg caggtggcgg ttcagctcac
      901 gcaccgacat ggacaccagc tggtcatcag agaagcggtc ctccacacta ccgttgccgc
      961 ccgcagccgt cgccgcggct gttgcgtgcg gtcccggccc cgggtggcta gtgggtagct
     1021 gttgcgcggg actggcagcg ctggacggcg ggggcgacgc ttggtgatga tggtgatggt
     1081 gcgggtgagc gtgctggccc aggtcatcgt gagtcacacc tgctcctggg tacccgtggt
     1141 gcggatgagg gtggtggtgg tggtgatggt ggtgcgcact acggaagccg tcgaagctct
     1201 gcagcggctg gggcactggg tgcgaaccga tgagcgcctc caccgcgtcc tcgggcgtca
     1261 ggttgagtgc ctcggggttc atctgctggt agttgctcgc catccagtac aggtcctcga
     1321 gatgggtctt cggttcagtc ggactgaagc tgggagaaga aggcacggag ctgcacggag
     1381 tgctgagcgg ggtggacgac accgagccag caggctgcag gcgtgtgcat ggccggcccg
     1441 gacgctccgc gcgccccagg ggctccttct tcacgtcgaa cttgagaagg tcgaagtcgt
     1501 tgacgtactc catggccagt gggctggtgg gcagctcttg ccccatgctc agctccgcgg
     1561 ccattaccgg tccaggcccg gggttttctt caacatcacc tgcttgcttt agcagagaga
     1621 agtttgtggc gccggatcct ctgtacttgt acagctcgtc catgccgaga gtgatcccgg
     1681 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     1741 gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     1801 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     1861 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     1921 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     1981 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     2041 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     2101 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cactgcaggc
     2161 cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     2221 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     2281 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     2341 gctcctcgcc cttgctcacc atggtggcgg cgcgccgcga taacttcgta taaagtatcc
     2401 tatacgaagt tatttgcctt aacccagaaa ttatcactgt tattctttag aatggtgcaa
     2461 agaataactt cgtataatgt atgctatacg aagttatgtt taaaccaatc aacctctgga
     2521 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2581 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2641 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2701 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2761 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2821 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2881 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     2941 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     3001 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3061 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc gagcttgtta
     3121 acatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     3181 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     3241 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     3301 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     3361 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     3421 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     3481 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     3541 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     3601 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     3661 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     3721 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt
     3781 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg
     3841 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4741 cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     4801 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4861 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4921 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4981 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5041 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5101 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5161 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5221 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5281 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5341 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5401 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5461 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5521 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5581 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5641 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatgatcc ggccattagc
     5701 catattattc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     5761 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
     5821 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     5881 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     5941 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     6001 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     6061 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     6121 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     6181 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     6241 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     6301 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     6361 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca
     6421 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtattccc
     6481 aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc
     6541 ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg
     6601 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
     6661 ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag
     6721 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc
     6781 ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga
     6841 ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag
     6901 aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc
     6961 gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt
     7021 ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt
     7081 cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt
     7141 tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc
     7201 acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat
     7261 ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct
     7321 ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
     7381 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
     7441 actccttctc taggcgccgg aattcgatgt cgacattgat tattgactag ttattaatag
     7501 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     7561 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     7621 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     7681 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     7741 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     7801 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat g
//
