LOCUS       sequence-183393-        1761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An ORF part-containing vector harbouring the signal peptide from 
            CelA with 4 bp overhangs specific for the 'signal peptide' position 
            within an ORF assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Gilbert C, Howarth M, Harwood CR, Ellis T
  TITLE     Extracellular Self-Assembly of Functional and Tunable Protein 
            Conjugates from Bacillus subtilis.
  JOURNAL   ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292.
  PUBMED    28230977
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292."
COMMENT     Sequence Label: pCG-A-celA(SP)-B
FEATURES             Location/Qualifiers
     source          1..1761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(116..704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(196..215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             886..1545
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(952..971)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1616..1650
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 tcctgagacc ccgactacgg ttatccacag aatcagggga taacgcagga aagaacatgt
       61 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      301 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      541 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      781 ttgatcgggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
      841 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      901 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      961 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     1021 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     1081 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
     1141 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     1201 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     1261 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtt
     1321 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     1381 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     1441 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     1501 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatttga tatcgagctc
     1561 gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag
     1621 aacgctcggt tgccgccggg cgttttttat tggtctggtc ggtctcagct tcctgctgat
     1681 gccggcgaag ctatcatgcc gcccattgtc aataacgtaa tcaataaaca cgtaataaaa
     1741 atagagattg accgtttcat a
//
