LOCUS       sequence-183395-        1956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An ORF part-containing vector harbouring the minimal SpyCatcher 
            (Delta-N1-Delta-C22) sequence with a His6 tag at its C-terminus and 
            4 bp overhangs specific for the 'downstream SpyPart' position within
            an ORF ass.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Gilbert C, Howarth M, Harwood CR, Ellis T
  TITLE     Extracellular Self-Assembly of Functional and Tunable Protein 
            Conjugates from Bacillus subtilis.
  JOURNAL   ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292.
  PUBMED    28230977
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292."
COMMENT     Sequence Label: pCG-D-mSpyCatcher-His6-E
FEATURES             Location/Qualifiers
     source          1..1956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     248..267
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      515..1103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1004..1023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1477..1494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(1525..1559)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1630..1956,1..333))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
       61 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
      121 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
      181 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
      241 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
      301 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
      361 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
      421 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
      481 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
      541 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      601 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      661 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      721 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      781 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      841 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      901 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      961 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1021 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1081 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1141 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1201 gtagtcgggg tctcaggcag cgactccgcg acacacatca aatttagcaa gcgtgatgag
     1261 gatgggaaag aattagccgg cgcaacgatg gaacttcggg attcgagcgg aaagacgatt
     1321 tcgacgtgga tttcagatgg ccaagtcaaa gatttttacc tgtatccggg aaaatataca
     1381 tttgtcgaaa cggcagcacc agatggatat gaggtcgcaa cagctattac atttacggtg
     1441 aatgagcaag gacaagtcac ggttaatggc ggaagccatc atcatcatca tcacggcagc
     1501 taagttctga gaccgaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1561 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     1621 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     1681 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1741 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1801 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
     1861 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     1921 atcttgcgaa tatatgtgta gaaactgccg gaaatc
//
