LOCUS       sequence-183402-        1923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An ORF part-containing vector harbouring the minimal SpyCatcher 
            (Delta-N1-Delta-C22) sequence with 4 bp overhangs specific for the 
            'upstream SpyPart' position within an ORF assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Gilbert C, Howarth M, Harwood CR, Ellis T
  TITLE     Extracellular Self-Assembly of Functional and Tunable Protein 
            Conjugates from Bacillus subtilis.
  JOURNAL   ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292.
  PUBMED    28230977
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292."
COMMENT     Sequence Label: pCG-B-mSpyCatcher-C
FEATURES             Location/Qualifiers
     source          1..1923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      43..631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     532..551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1020..1054)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1125..1784)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1699..1718
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
       61 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      121 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      181 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      301 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      361 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      421 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      481 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      541 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      601 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      661 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      721 ggataaccgt agtcggggtc tcaaagcgat tcagctacgc atataaaatt ttctaaacgg
      781 gacgaagacg gcaaggaact ggcgggtgct actatggaac tccgggactc atcgggaaaa
      841 actataagca cgtggatcag cgatggacaa gtcaaggact tttaccttta ccctggcaag
      901 tacacattcg tcgaaacggc tgctccggat ggatatgaag tggcaacagc tattacattc
      961 acggttaacg aacaggggca ggtcaccgtg aacgggggct catgagaccg accagaccaa
     1021 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     1081 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     1141 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     1201 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     1261 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     1321 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
     1381 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     1441 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     1501 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     1561 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     1621 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     1681 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1741 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1801 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1861 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     1921 gtt
//
