LOCUS       sequence-183474-       13732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of Luciferase with 3xFLAG
            N-terminal tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 13732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pSLIK TT 3xFLAG Luciferase neo
FEATURES             Location/Qualifiers
     source          1..13732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        171..550
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        552..750
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             768..948
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    995..1120
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1613..1846
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2031..2075
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2373..2490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2603..2917
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2611..2629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2647..2665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2682..2700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2718..2736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2754..2772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2789..2807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2825..2843
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2890..2914
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2927..2992
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(3059..3077)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(4675..4699)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4749..5960
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5797..5816
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6019..6636,6637..6642,6643..6678,6679..6681,6682..6717,
                     6718..6720,6721..6756,6757..6765)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6803..7375
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6969..6986)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7196..7215
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7391..8185
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7391..8185
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7445..7464)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8055..8074
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8353..8941
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8406..8426)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8824..8835)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8944..8960)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8945..8961)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9470..9650
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9676..9693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9682..9906
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9952..10380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10039..10058)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10249..10270
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10389..10409)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10394..10723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10574..10709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10636..10655
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10771..10818
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10837..11211
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11341..11462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11378..11397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11432..11451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11511..11527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11511..11527)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11524..11546)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11535..11551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11559..11565,11566..11583,11584..11589))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11604..11625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11742..11759)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11913..12501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11993..12012)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12672..13463,13464..13532))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13295..13314
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13533..13637)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13712..13731)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
       61 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      121 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      181 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      241 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      301 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      361 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      421 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      481 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      541 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      601 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      661 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      721 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      781 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      841 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      901 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      961 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     1021 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1081 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1141 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1201 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1261 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1321 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1381 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1441 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1501 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1561 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1621 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1681 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1741 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1801 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1861 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1921 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1981 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2041 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2101 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2161 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2221 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2281 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2341 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2401 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2461 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2521 gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc tttaaaggaa
     2581 ccaattcagt cgactggatc ccgagtttac tccctatcag tgatagagaa cgtatgtcga
     2641 gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca gtgatagaga
     2701 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2761 cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg tatgtcgagt
     2821 ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggaggccta
     2881 tataagcaga gctcgtttag tgaaccgtca gatcgccact agtatggact acaaagacca
     2941 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca agatggaaga
     3001 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
     3061 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     3121 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
     3181 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     3241 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     3301 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
     3361 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
     3421 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     3481 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
     3541 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
     3601 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
     3661 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     3721 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     3781 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     3841 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     3901 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     3961 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     4021 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     4081 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     4141 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     4201 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     4261 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     4321 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     4381 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     4441 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     4501 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     4561 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     4621 gaagggcgga aagatctgag aattggaaga ctaatgcgtt ttcgagatat ctagacccag
     4681 ctttcttgta caaagtggtt gataacatct cgagccataa agatggttaa ttaacccacc
     4741 caagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
     4801 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
     4861 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
     4921 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
     4981 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
     5041 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     5101 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     5161 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     5221 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     5281 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     5341 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     5401 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     5461 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     5521 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     5581 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     5641 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     5701 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     5761 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     5821 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     5881 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     5941 ttttggcttt tttgttagac gaagcttggt accgagctcg gatctccacc ccgtaccggt
     6001 cctgcagtcg aattcaccat gtctagactg gacaagagca aagtcataaa cggagctctg
     6061 gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg
     6121 ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc
     6181 ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca
     6241 tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc
     6301 gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa
     6361 aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg
     6421 tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca
     6481 aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt
     6541 gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata
     6601 tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat
     6661 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
     6721 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatccg
     6781 cggccgcact agaggaattc cgcccctctc cccccccccc cctaacgtta ctggccgaag
     6841 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6901 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     6961 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     7021 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     7081 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     7141 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     7201 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     7261 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     7321 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagctta
     7381 ccggtccacc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     7441 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     7501 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     7561 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     7621 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     7681 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     7741 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     7801 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7861 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     7921 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7981 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     8041 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     8101 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     8161 tcgccttctt gacgagttct tctgatgtac aagtaaagcg gccgcgactc tagatcataa
     8221 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     8281 tgaacctgaa acataaaatg aatgcaattg ttgttgttta gtccctccca attcgatatc
     8341 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8401 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8461 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8521 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8581 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8641 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8701 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8761 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8821 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8881 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8941 catcgatacc gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa
     9001 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     9061 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     9121 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     9181 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     9241 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     9301 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     9361 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     9421 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     9481 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9541 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9601 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     9661 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     9721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     9781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     9841 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     9901 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     9961 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    10021 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    10081 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    10141 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    10201 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    10261 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    10321 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    10381 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    10441 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    10501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    10561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
    10681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
    10741 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
    10801 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
    10861 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
    10921 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
    10981 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
    11041 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
    11101 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
    11161 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
    11221 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    11281 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    11341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11461 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
    11521 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11581 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    11641 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    11701 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    11761 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    11821 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    11881 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    11941 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    12001 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    12061 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    12121 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12181 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12241 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12301 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    12361 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12421 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12481 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12541 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12601 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12661 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12721 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    12781 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    12841 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    12901 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    12961 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    13021 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    13081 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    13141 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    13201 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13261 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    13321 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13381 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13441 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13501 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13561 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13621 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    13681 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gc
//
