LOCUS       sequence-183493-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for inducible knockdown of LacZ (control 
            hairpin).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: TET-pLKO.1 PURO shLacZ
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    163..736
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(330..347)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     557..576
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             756..1355
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(756..775)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1252..1272
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1483..1716
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1788..1909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1825..1844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1879..1898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1949..2084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2105..2123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2133..2150)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2133..2149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2142..2164)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2291..2746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2378..2397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2588..2609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2772..2876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2877..2945,2946..3737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3095..3114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3908..4496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4397..4416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4650..4667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4784..4805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4820..4825,4826..4843,4844..4850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4858..4874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4863..4885
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4882..4898
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4882..4898
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4917..4937
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4919..4937
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4965..5191
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5192..5372
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5419..5544
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6037..6270
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6455..6499
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6788..6882
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     6799..6818
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    6836..6854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6864..6882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    6983..7100
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7149..7659
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7578..7596
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          7668..8240
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     8248..8267
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        8295..8313
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(8324..8816,1..128)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(8397..8416)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
       61 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
      121 gtgggtccgc gtacagcggc tcccgggagt tctagggatc tgcccctctc cctccccccc
      181 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
      241 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
      301 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
      361 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
      421 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
      481 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
      541 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
      601 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
      661 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
      721 aaaaacacga tgataaggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc
      781 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
      841 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
      901 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
      961 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     1021 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     1081 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     1141 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     1201 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     1261 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     1321 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     1381 cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg acttacaagg
     1441 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     1501 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     1561 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1621 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1681 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     1741 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     1801 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1861 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     1921 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1981 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2041 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     2101 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     2161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2281 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2341 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2401 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2461 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2521 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2581 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2641 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2701 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     2761 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2821 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2881 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2941 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3001 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3061 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3121 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3181 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3241 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3301 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3361 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3421 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3481 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3541 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3601 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3661 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3721 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3781 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3841 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3901 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3961 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4021 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4081 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4141 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4201 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4261 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4321 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4381 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4441 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4501 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4561 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4621 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4681 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4741 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     4801 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     4861 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     4921 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     4981 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     5041 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     5101 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     5161 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     5221 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5281 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5341 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     5401 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     5461 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     5521 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     5581 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     5641 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     5701 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     5761 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     5821 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     5881 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     5941 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     6001 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     6061 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     6121 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     6181 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     6241 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     6301 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     6361 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     6421 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     6481 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     6541 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     6601 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     6661 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     6721 cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact agcctcgagc
     6781 ggccgcaata tttgcatgtc gctatgtgtt ctgggaaatc accataaacg tgaaatccct
     6841 atcagtgata gagacttata agttccctat cagtgataga gacaccgggt tccgtcatag
     6901 cgataacgac tgcagtcgtt atcgctatga cggaactttt tgaattctcg acctcgagac
     6961 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     7021 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     7081 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc
     7141 ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     7201 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
     7261 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     7321 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     7381 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     7441 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     7501 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     7561 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     7621 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatctgtg agtttgggga
     7681 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag
     7741 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
     7801 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
     7861 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
     7921 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
     7981 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
     8041 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
     8101 acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt
     8161 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
     8221 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     8281 ttggcaaaga attgtaatac gactcactat agggcgaatt gatatgtcta gattagataa
     8341 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
     8401 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
     8461 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
     8521 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
     8581 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
     8641 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
     8701 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
     8761 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgata
//
