LOCUS       sequence-18350-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Adam RM, Kim J, Lin J, Orsola A, Zhuang L, Rice DC, Freeman MR
  TITLE     Heparin-binding epidermal growth factor-like growth factor 
            stimulates androgen-independent prostate tumor growth and 
            antagonizes androgen receptor function.
  JOURNAL   Endocrinology. 2002 Dec . 143(12):4599-608.
  PUBMED    12446587
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology. 2002 Dec . 143(12):4599-608."
COMMENT     Sequence Label: pcDNA3 proHB-EGF CS
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             475..504
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             520..537
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(560..577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    566..677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgagctcgga tcctgaagct gctgccgtcg gtggtgctga agctctttct ggctgcagtt
       61 ctctcggcac tggtgactgg cgagagcctg gagcggcttc ggagagggct agctgctgga
      121 accagcaacc cggaccctcc cactgtatcc acggaccagc tgctacccct aggaggcggc
      181 cgggaccgga aagtccgtga cttgcaagag gcagatctgg accttttgag agtcacttta
      241 tcctccaagc cacaagcact ggccacacca aacaaggagg agcacgggaa aagaaagaag
      301 aaaggcaagg ggctagggaa gaagagggac ccatgtcttc ggaaatacaa ggacttctgc
      361 atccatggag aatgcaaata tgtgaaggag ctccgggctc cctcctgcat ctgccacccg
      421 ggttaccatg gagagaggtg tcatgggctg agcctcccag tggaagggcc cttcgaacaa
      481 aaactcatct cagaagagga tctgaatatg cataccggtc atcatcacca tcaccattga
      541 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
      661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctatctgggg g
//
