LOCUS       sequence-18352-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Adam RM, Kim J, Lin J, Orsola A, Zhuang L, Rice DC, Freeman MR
  TITLE     Heparin-binding epidermal growth factor-like growth factor 
            stimulates androgen-independent prostate tumor growth and 
            antagonizes androgen receptor function.
  JOURNAL   Endocrinology. 2002 Dec . 143(12):4599-608.
  PUBMED    12446587
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology. 2002 Dec . 143(12):4599-608."
COMMENT     Sequence Label: pIRES proHB-EGF CS
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             564..593
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(603..620)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctcggatc catgaagctg ctgccgtcgg
      121 tggtgctgaa gctctttctg gctgcagttc tctcggcact ggtgactggc gagagcctgg
      181 agcggcttcg gagagggcta gctgctggaa ccagcaaccc ggaccctccc actgtatcca
      241 cggaccagct gctaccccta ggaggcggcc gggaccggaa agtccgtgac ttgcaagagg
      301 cagatctgga ccttttgaga gtcactttat cctccaagcc acaagcactg gccacaccaa
      361 acaaggagga gcacgggaaa agaaagaaga aaggcaaggg gctagggaag aagagggacc
      421 catgtcttcg gaaatacaag gacttctgca tccatggaga atgcaaatat gtgaaggagc
      481 tccgggctcc ctcctgcatc tgccacccgg gttaccatgg agagaggtgt catgggctga
      541 gcctcccagt ggaagggccc ttcgaacaaa aactcatctc agaagaggat ctgaatatgc
      601 atatggtgat ggtgatgatg accggtatgc atctagggcg gccgcactag agg
//
