LOCUS       sequence-18355-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Kim J, Adam RM, Freeman MR
  TITLE     Trafficking of nuclear heparin-binding epidermal growth factor-like 
            growth factor into an epidermal growth factor receptor-dependent 
            autocrine loop in response to oxidative stress.
  JOURNAL   Cancer Res. 2005 Sep 15. 65(18):8242-9.
  PUBMED    16166300
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2005 Sep 15. 65(18):8242-9."
COMMENT     Sequence Label: pcDNA3 proHB-EGF NC
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..59)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(722..741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(723..741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(786..885)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(815..835)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgatggtgat gatgaccggt atgcatattc agatcctctt ctgagatgag tttttgttcg
       61 aaccgcgggc ccgtgggaat tagtcatgcc caacttcact ttctcttcat tttccacatc
      121 ataacctcct ctcctatggt acctaaacat gagaagcccc acgatgacca gcagacagac
      181 agatgacagc accacagcca ccacggccag gatggttggc tgcaccgggg tggaccatgt
      241 gggcagaacc ttgaggctca gcccatgaca cctctctcca tggtaacccg ggtggcagat
      301 gcaggaggga gcccggagct ccttcacata tttgcattct ccatggatgc agaagtcctt
      361 gtatttccga agacatgggt ccctcttctt ccctagcccc ttgcctttct tctttctttt
      421 cccgtgctcc tccttgtttg gtgtggccag tgcttgtggc ttggaggata aagtgactct
      481 caaaaggtcc agatctgcct cttgcaagtc acggactttc cggtcccggc cgcctcctag
      541 gggtagcagc tggtccgtgg atacagtggg agggtccggg ttgctggttc cagcagctag
      601 ccctctccga agccgctcca ggctctcgcc agtcaccagt gccgagagaa ctgcagccag
      661 aaagagcttc agcaccaccg acggcagcag cttcataagc ttaactagcc agcttgggtc
      721 tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc
      781 cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
      841 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
      901 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctat
//
