LOCUS       sequence-183609-       13978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in 
            zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Ki DH, He S, Rodig S, Look AT
  TITLE     Overexpression of PDGFRA cooperates with loss of NF1 and p53 to 
            accelerate the molecular pathogenesis of malignant peripheral nerve 
            sheath tumors.
  JOURNAL   Oncogene. 2017 Feb 23;36(8):1058-1068. doi: 10.1038/onc.2016.269. 
            Epub 2016 Aug 1.
  PUBMED    27477693
REFERENCE   2  (bases 1 to 13978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2016.269"; journalName: "Oncogene"; date: "2017-02-23- 
            23"; volume: "36"; issue: "8"; pages: "1058-1068"
COMMENT     Sequence Label: sox10:PDGFRA wild-type
FEATURES             Location/Qualifiers
     source          1..13978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2847..2871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6148..6172)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6174..6308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6371..6391)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6399..6415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6431..6565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6699..6717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6750..6766)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6759..6781)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6908..7363
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6995..7014)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7205..7226
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7389..7493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7494..7562,7563..8354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7712..7731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8525..9113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9014..9033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9267..9284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9401..9422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9437..9442,9443..9460,9461..9467)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9475..9491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9480..9502
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9499..9515
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9557..9577
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9559..9577
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9614..9630
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9614..9630
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9664..9680)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9683..9699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9719..9739
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aatgtaaggc aaaggaggat tatttaaata gaatgtcata cacaatggaa aatcaaagtg
       61 ttttacataa acaacaataa aagaaacata aataacaagc gtaaaagcaa attcaaactg
      121 aataataaaa aaataataaa gttgaagaca caaatgtata tttaaaaaag tttaagatct
      181 gaattaaaag taagatgccc ctgtgagagg ccaaatatta ctgtcgttga tttgcagccg
      241 aaatcaccgc cctctggggc aaaactcagc agccaatcag agcgggaccc gacagggctc
      301 tgttgctcct ttaagagatt tttttccatg aattgatttt tttctcgctc ttcacacaac
      361 ggggctcttt aagcctcgac gcgcgacaca gagcaggcat tcagagcgcg agcgaggggg
      421 ctgaaccgac ggactctcgt gctgggcggg cgacctgctg cactgtaaag taagtgcatt
      481 actggctttc tctctatcgc ttactctgtg tgggaatata tacgccattg cactgtctaa
      541 cggagaaact tttacatttc ttattagtaa taatactact aataataagc ggtgcattta
      601 taaacgacat ctacttattt tggatttttc ggtttatgta ttgatgggtt tggtcgtgga
      661 taattgtaat ggagaaggtt tttaattgaa atggattaaa ggacgtcttt aaaagtgcaa
      721 cttatctaat cttttggcga aaggaacact taaagttggt gtcacggggc ataaatttcg
      781 agatttgcgt gaatgtgtaa atcagagacg tgttgggtta atctgtctta attggaaaga
      841 acggcttaat ctatctgtgc acggaccact tgggatgtac atacactttt agatctttta
      901 gtaaatgttt acagtccgat aaattataca tttgaacact ttaattcgaa gtgaatcagt
      961 ttttaaatag ttttataaga aataattttt ttaaatttaa tcttctgaga agttttactt
     1021 gcgtaaggct atttgtatat atcgccgtct ctcgccaaat tgctcatgta accaacgcaa
     1081 agattaacaa agagaccaaa ctttaaagta cacaatttat aatattgttt atctacacat
     1141 cctttcacaa caaatataac aggatcagaa aacttaagta aagtaaaact aactgaaatg
     1201 cacatttgtg taagtttgaa tcaaatgttt gatacaataa taatctccgc agattagttt
     1261 gaaaggtttc attcgaatga gtttgtgtca tttatgagtt ttgtgttttg gcatgagtga
     1321 attaggggca aattattgcc aaaccaggcg ttatgatccg ccctacagca gccaaagatg
     1381 aacccaatca aatctgaaca aataaagttt acagcggagg aaactcaaat aagcaagagt
     1441 tgcgagcaaa gttaaaatga atgagcataa agtttataaa agtcctcctg acagaaaaca
     1501 gccgctgaaa tcttttcctt aaaacaaaat aaacccagca gcgcaattac cgaaattgac
     1561 ggatacattt gttagctact aaaacaaacc aagtcttgtg cgatgctttc tttttcgtcc
     1621 caaataaatt ggacaagtca aaccattaac gctttctgta ggctatacat gtgattaaca
     1681 aatgatcaat gtgttaatgt gttttgtaag gggtctacaa gtagccattc ttggctaact
     1741 ctagcacatt ttacagtaat gtttattaat gtgcattgac cctgtaaaca aataaactaa
     1801 acatggttct gctcatgtga agtcacctgt atcttaatta acactgctgt tggatgctgt
     1861 gtttttgtat tcgggttaat attggatgtc tgtaactcaa gttcaagctg acaggtcgca
     1921 tctcgacagc ttctgtcgca tataaattat ttgatcgatt tttctgtaat tggacctagt
     1981 caaccacatc cactcgttta attcacgatt acttcacatt tcagaaacac actgccatga
     2041 ctctcttgat ttttttcttc ttctgctggt tataagtcgt cgtttgtgct ttcaccaaca
     2101 aacactgtgt aattcagctg cccacggcag cgccgctccg tgtttatcca gcagcgaggc
     2161 ggaatgaagc cggtattatt ccagcacttt ctctcccggg agcttgaaca aaccgccaaa
     2221 ccttcaacag tagcttgtta gcagaagtga agctaaaata acacacacac atatacacac
     2281 accgaggccc actgacgcca ccgtatgatt gcagcgctgc cctggaaaaa ggcgacttct
     2341 aaataaagtc aaagccttag tgtttgtagg tttttcctga acaggtttat cttgttcatg
     2401 aggtgttcgg gtgaacagca aaggggttgt gcggtcatac agccaaccat taaaacgaca
     2461 tattaatgct attttataca ttaggaagac atgctctgca gaagcaagct gtttaataaa
     2521 ataataacaa tgattattat aactaataaa ttacctgtgt gtgtattttt gtaggcttcc
     2581 atcagatcta tatccagagg aagacggcgg aaggattcct tcttacaaac tcgaattata
     2641 taaaaagaac tgttaaggtt tcactggatg atcttaaata ataaacaaaa gcacaattat
     2701 tttacaagaa aaaaacattt gtaaagtata aattaataca tttatattta aaataaaatt
     2761 taagtgagga aattaaacct accgaagtca cctgtggccg cagaactagt tctagaacta
     2821 tagtgagtcg tattacgtag atccagacaa gtttgtacaa aaaagcaggc tccaccatgg
     2881 ggacttccca tccggcgttc ctggtcttag gctgtcttct cacagggctg agcctaatcc
     2941 tctgccagct ttcattaccc tctatccttc caaatgaaaa tgaaaaggtt gtgcagctga
     3001 attcatcctt ttctctgaga tgctttgggg agagtgaagt gagctggcag taccccatgt
     3061 ctgaagaaga gagctccgat gtggaaatca gaaatgaaga aaacaacagc ggcctttttg
     3121 tgacggtctt ggaagtgagc agtgcctcgg cggcccacac agggttgtac acttgctatt
     3181 acaaccacac tcagacagaa gagaatgagc ttgaaggcag gcacatttac atctatgtgc
     3241 cagacccaga tgtagccttt gtacctctag gaatgacgga ttatttagtc atcgtggagg
     3301 atgatgattc tgccattata ccttgtcgca caactgatcc cgagactcct gtaaccttac
     3361 acaacagtga gggggtggta cctgcctcct acgacagcag acagggcttt aatgggacct
     3421 tcactgtagg gccctatatc tgtgaggcca ccgtcaaagg aaagaagttc cagaccatcc
     3481 catttaatgt ttatgcttta aaagcaacat cagagctgga tctagaaatg gaagctctta
     3541 aaaccgtgta taagtcaggg gaaacgattg tggtcacctg tgctgttttt aacaatgagg
     3601 tggttgacct tcaatggact taccctggag aagtgaaagg caaaggcatc acaatactgg
     3661 aagaaatcaa agtcccatcc atcaaattgg tgtacacttt gacggtcccc gaggccacgg
     3721 tgaaagacag tggagattac gaatgtgctg cccgccaggc taccagggag gtcaaagaaa
     3781 tgaagaaagt cactatttct gtccatgaga aaggtttcat tgaaatcaaa cccaccttca
     3841 gccagttgga agctgtcaac ctgcatgaag tcaaacattt tgttgtagag gtgcgggcct
     3901 acccacctcc caggatatcc tggctgaaaa acaatctgac tctgattgaa aatctcactg
     3961 agatcaccac tgatgtggaa aagattcagg aaataaggta tcgaagcaaa ttaaagctga
     4021 tccgtgctaa ggaagaagac agtggccatt atactattgt agctcaaaat gaagatgctg
     4081 tgaagagcta tacttttgaa ctgttaactc aagttccttc atccattctg gacttggtcg
     4141 atgatcacca tggctcaact gggggacaga cggtgaggtg cacagctgaa ggcacgccgc
     4201 ttcctgatat tgagtggatg atatgcaaag atattaagaa atgtaataat gaaacttcct
     4261 ggactatttt ggccaacaat gtctcaaaca tcatcacgga gatccactcc cgagacagga
     4321 gtaccgtgga gggccgtgtg actttcgcca aagtggagga gaccatcgcc gtgcgatgcc
     4381 tggctaagaa tctccttgga gctgagaacc gagagctgaa gctggtggct cccaccctgc
     4441 gttctgaact cacggtggct gctgcagtcc tggtgctgtt ggtgattgtg atcatctcac
     4501 ttattgtcct ggttgtcatt tggaaacaga aaccgaggta tgaaattcgc tggagggtca
     4561 ttgaatcaat cagcccggat ggacatgaat atatttatgt ggacccgatg cagctgcctt
     4621 atgactcaag atgggagttt ccaagagatg gactagtgct tggtcgggtc ttggggtctg
     4681 gagcgtttgg gaaggtggtt gaaggaacag cctatggatt aagccggtcc caacctgtca
     4741 tgaaagttgc agtgaagatg ctaaaaccca cggccagatc cagtgaaaaa caagctctca
     4801 tgtctgaact gaagataatg actcacctgg ggccacattt gaacattgta aacttgctgg
     4861 gagcctgcac caagtcaggc cccatttaca tcatcacaga gtattgcttc tatggagatt
     4921 tggtcaacta tttgcataag aatagggata gcttcctgag ccaccaccca gagaagccaa
     4981 agaaagagct ggatatcttt ggattgaacc ctgctgatga aagcacacgg agctatgtta
     5041 ttttatcttt tgaaaacaat ggtgactaca tggacatgaa gcaggctgat actacacagt
     5101 atgtccccat gctagaaagg aaagaggttt ctaaatattc cgacatccag agatcactct
     5161 atgatcgtcc agcctcatat aagaagaaat ctatgttaga ctcagaagtc aaaaacctcc
     5221 tttcagatga taactcagaa ggccttactt tattggattt gttgagcttc acctatcaag
     5281 ttgcccgagg aatggagttt ttggcttcaa aaaattgtgt ccaccgtgat ctggctgctc
     5341 gcaacgtcct cctggcacaa ggaaaaattg tgaagatctg tgactttggc ctggccagag
     5401 acatcatgca tgattcgaac tatgtgtcga aaggcagtac ctttctgccc gtgaagtgga
     5461 tggctcctga gagcatcttt gacaacctct acaccacact gagtgatgtc tggtcttatg
     5521 gcattctgct ctgggagatc ttttcccttg gtggcacccc ttaccccggc atgatggtgg
     5581 attctacttt ctacaataag atcaagagtg ggtaccggat ggccaagcct gaccacgcta
     5641 ccagtgaagt ctacgagatc atggtgaaat gctggaacag tgagccggag aagagaccct
     5701 ccttttacca cctgagtgag attgtggaga atctgctgcc tggacaatat aaaaagagtt
     5761 atgaaaaaat tcacctggac ttcctgaaga gtgaccatcc tgctgtggca cgcatgcgtg
     5821 tggactcaga caatgcatac attggtgtca cctacaaaaa cgaggaagac aagctgaagg
     5881 actgggaggg tggtctggat gagcagagac tgagcgctga cagtggctac atcattcctc
     5941 tgcctgacat tgaccctgtc cctgaggagg aggacctggg caagaggaac agacacagct
     6001 cgcagacctc tgaagagagt gccattgaga cgggttccag cagttccacc ttcatcaaga
     6061 gagaggacga gaccattgaa gacatcgaca tgatggacga catcggcata gactcttcag
     6121 acctggtgga agacagcttc ctgtgatacc cagctttctt gtacaaagtg ggggatccag
     6181 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     6241 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     6301 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     6361 aggttttttc caactttatt atacatagtt gataattcac tggccgtcgt tttacggtac
     6421 catcgatgat gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6481 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6541 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     6601 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     6661 tgattatgat cctctagatc agatcgtacc caattcgccc tatagtgagt cgtattacgc
     6721 gcgcattacc ctgttatccc tacgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     6781 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6841 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6901 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6961 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7021 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7081 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7141 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7201 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7261 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7321 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     7381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7681 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8341 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8641 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9181 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9241 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9301 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9361 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9421 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9481 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgtaggga
     9541 taacagggta atgcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctccaccg
     9601 cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct
     9661 tatcgatacc gtcgacctcg agcaggaaac agctatgacc atgattacgc caagctatca
     9721 actttgtata gaaaagttgt acgccagtcg acggtatcga taagcttgat atcgaattcc
     9781 ccacagcgga caacaggaca aaacctctca ctgctacatg atcaatcctc cacccactgc
     9841 ccgctgcact tcctgtctca cacacacaca cacacacaag cagagccagt gccatcaggt
     9901 gtgtgtgtgt gtgtgtcatg ggacatgcgt gtcctcctat tgaccttctg acctcacaaa
     9961 catctttctc tgacatccca gagaccatct ccaacggcaa ccaaccacat catcactgtc
    10021 acatgctgca tgactgcaca cacacacaca cacactttct cgctcacaca aacatattga
    10081 ttcacattta tacacacatt taaacacaca aacacattta aatacacaca cacacacaca
    10141 cacacacatg atcctataaa ctgcgcacaa cactaagcac cagcagtttt agtttgctga
    10201 agcatcagtt tgtaacagaa taactcagcc tcctcatgat ctcctcttcg ctgctcattc
    10261 aggcaaaact gcagtttaaa agcgctgata aacaaaactc caactatggc acacacactc
    10321 ccttgatcag agatgaattg atgaaattaa ttatgttttt gtgtgcgtat ttctttatta
    10381 aatcacgcag acgcgcgcag ttttatctct gcggtaatgc ggatcataat aaactcgtga
    10441 ttaaaaaact aattacactg tcgcacgaac atgcgcgcat taacaacgaa cacacgagtc
    10501 atttcgataa atatacagct cagaaattac attaaacatt tataagatat caaaaacggt
    10561 caagagaaac gaatttattt atgtttttat aaagcgcatt tgcgagtcgc tcaaaaaact
    10621 gtatagtatg aacataataa tgtttaaaaa acacgaaata ctgatacaaa taatactgtt
    10681 aataataaaa acattgttat ttgtattaaa tagcttgtgg atatccagaa ataatgcgta
    10741 attctaaccg acagatgatc taaaatcatt tcgtcagtct cttatttgat agactacaaa
    10801 tgcataaaaa gtaaaacgcg ttttcgtatt tattaaattg gttttagttt tttttaatta
    10861 aattataata ttataataaa ataatcaatt gtatttttat tactgacatg cacgcataat
    10921 aaacgcgcta tacaaatgcg catacacatt acgcacctgc gcgtttgggt tatttccatt
    10981 taaataaatt caactacaaa accccaaaac aaaaagttta aaaacatttt aatttctgaa
    11041 tttaaaaaat aaacaaaatc aatcatttaa aaattgtagg cagggttgta ttaacccaac
    11101 gctgggtcaa atatggactc aaagtttttg catggcattt aaatatttaa tccgtctgct
    11161 gaatttgtcc atatttgaca ataatacatc ccagctattt tagagcgcat ttctgatctc
    11221 ttctttacca taaaaacgaa accccttgat aaagcagaat ggcattaaac tggaaattaa
    11281 taataatgta aacaattaac taataacata tataaaacaa aacgatttac acaaatagcg
    11341 caggacacat ttccgcggta taaaagaagc ctccctgttc tgacaggcga tgcgacacac
    11401 ataacaaatc actcttctca agcagccaca ttaaataaca gaagaaacaa ttaacatgca
    11461 cgcgcatact aaacagcaca cattaacaaa tgtccctcac tgacaaacac cccccagctt
    11521 gatgtttatg tcctataaat gctcggagac cgagtgtcac tttctccaaa gcggggtctc
    11581 ctccttccca aagcctccct aagagcaaca gggcaaaact cacatcaaag tgtgcagcag
    11641 gcctcagatc cgcgcgcttt cccgtgtaca tccacagacc cgcgagaacc gagacatgtg
    11701 tgcgtgtacg tttgaaggga caccgagaaa aagacgctct aaagagagac acacgtcttt
    11761 tctcttttct tttctttttt tatatacaca gaactgcttt ttgttcctca ttttttcatt
    11821 taagagggta atggaagggg ggtgttcgtt tgatcacatg attacaattc acctgagata
    11881 aaacaaagga cgcaaagaaa ggagccgagc gccggagacc gagacagaga gatgctctca
    11941 tggtcaggat ccccttatca gagtcaacat tcatggataa tgaaaaaggc aatgattttt
    12001 ggcttgggtg ggggggccgt cgtgagatgg aaatacaaga ggttattgtt gaccagaaca
    12061 gcgcgcgtgt ttgtgtgcgt ttggcccgtc gtttgcctct cttctcgtct ttctatattt
    12121 tctactaaag ctgtttaaac gcgttaaatg cgctctctgt gtgtgtttat gcgcgctgat
    12181 gcaattataa cactaacaca catagcgcga cacgatgcag gagttcattc cagtgctgcg
    12241 ggcctgcgcg cgcagcatga acacaccact gcgagcaaat ctgcaatatt aatgaatata
    12301 tataactaaa gtgtgcactt tctggtttat aactgcagca ccccgcatac accacagtat
    12361 aaagtaatct acaatattaa tgaatataat aaacaagaat gttcactttt aggtttaaga
    12421 atgcaggcct gcgcgcgcag catcccgcga gcacaacact agaaactaaa ctccaatatt
    12481 agttaatata ttcaattaaa atgtacactt tctggtttaa aactgcaagt atgcgtgcgc
    12541 agcatccgac atacccaaca ctagaaacta atctacaata tttatgaaag tattcaacta
    12601 aaatgttcac tttctggttt aaaactgcaa gtatacgcgt gcgcagcatc cgacataccc
    12661 aacactagaa attaatctaa attattaatt aaagtattca actgaattgt tcactttctg
    12721 gtttaaaact gcaagtatgg gtgcgcagca taaaacatac ccaacactag aaactaatct
    12781 acaatattaa tggatgtatt caactaaaat gttcactttc tggtttaaaa ctgcaggaat
    12841 gcgcgcgcaa caccctgcgg ataactttaa aaacaattcc gcaatcagct aaaactgaaa
    12901 agttcagttg attaacgaat gtaggcatgc gcaaaatatt ataaatgcgc acacacatct
    12961 atttaataca ttaaaactaa ggctaaatgt tcactttcac cattttgggg aaaatgaaaa
    13021 ttcagtgtcc cccttaagtg gccagcttta gggcaatagg cgaccctcca taagtagcat
    13081 aaaatcgtag gctattctgg gcaccctttg gcatcgccac gcgttaaaca gggaatggag
    13141 aggttattgc gcacagcagg tgcgcagagt cgcgtccgct aatggcgctg gagctgctga
    13201 caggtccgct ggcgctctgc ggggtggaag cgctcgaaac cccgcacaac ccctgattca
    13261 ccctgaggca cagccaggcc aacaacgggc cagtgtgatc ggaaaagagt tgaaagaatc
    13321 tccgtgacac caatgaggag ccaaagacca cctgtgaaat acatcacatg tcagacaaaa
    13381 gcatattaag ctgatcaaat attgcgtgca taaccgcatt acatcacaaa gaaaaacatt
    13441 agctagctat gggtaactta ataagtgttc tacatttgca ctgcagccat ggttgtgagc
    13501 cgaaaaggtt tgcgcaataa tattcacccc ccgagattta catttttttt ttgcggttct
    13561 ctgatgtcga ttaatggcca cgtggccttc aggtaaacac atttaatatt taacttatgt
    13621 gttttaatta gtgtgttccg aattattcgg ttatttttat ttaacatttt tattaattag
    13681 cttaattctg atccactccc aactcttatt aacgaatcac aaatcaaatg gatagtttta
    13741 attgcacaat tagcctgtca aaacttaact taaagctgtt aatgcactgc ttttaagttt
    13801 gaaaaataaa gaaaaattat tagaaaactt aagattagat tttaacatat gttgaaatta
    13861 tcaaatcggc atgtttatga taaaacaact ttacctaaaa ctaaaattca gatcaacaaa
    13921 aacctaaatg aagttacata tatatatata tgtgtgtgtg tgtgtgtata tatatata
//
