LOCUS       sequence-183612-       13735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  I-SceI meganuclease-mediated human PDGFRA mutant transgenesis in 
            zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Ki DH, He S, Rodig S, Look AT
  TITLE     Overexpression of PDGFRA cooperates with loss of NF1 and p53 to 
            accelerate the molecular pathogenesis of malignant peripheral nerve 
            sheath tumors.
  JOURNAL   Oncogene. 2017 Feb 23;36(8):1058-1068. doi: 10.1038/onc.2016.269. 
            Epub 2016 Aug 1.
  PUBMED    27477693
REFERENCE   2  (bases 1 to 13735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2016.269"; journalName: "Oncogene"; date: "2017-02-23- 
            23"; volume: "36"; issue: "8"; pages: "1058-1068"
COMMENT     Sequence Label: sox10:PDGFRA mutant
FEATURES             Location/Qualifiers
     source          1..13735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2847..2871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5905..5929)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5931..6065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6128..6148)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6156..6172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6188..6322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6456..6474)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6507..6523)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6516..6538)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6665..7120
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6752..6771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6962..6983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7146..7250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7251..7319,7320..8111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7469..7488)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8282..8870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8771..8790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9024..9041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9158..9179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9194..9199,9200..9217,9218..9224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9232..9248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9237..9259
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9256..9272
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9314..9334
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9316..9334
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9371..9387
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9371..9387
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9421..9437)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9440..9456
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9476..9496
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aatgtaaggc aaaggaggat tatttaaata gaatgtcata cacaatggaa aatcaaagtg
       61 ttttacataa acaacaataa aagaaacata aataacaagc gtaaaagcaa attcaaactg
      121 aataataaaa aaataataaa gttgaagaca caaatgtata tttaaaaaag tttaagatct
      181 gaattaaaag taagatgccc ctgtgagagg ccaaatatta ctgtcgttga tttgcagccg
      241 aaatcaccgc cctctggggc aaaactcagc agccaatcag agcgggaccc gacagggctc
      301 tgttgctcct ttaagagatt tttttccatg aattgatttt tttctcgctc ttcacacaac
      361 ggggctcttt aagcctcgac gcgcgacaca gagcaggcat tcagagcgcg agcgaggggg
      421 ctgaaccgac ggactctcgt gctgggcggg cgacctgctg cactgtaaag taagtgcatt
      481 actggctttc tctctatcgc ttactctgtg tgggaatata tacgccattg cactgtctaa
      541 cggagaaact tttacatttc ttattagtaa taatactact aataataagc ggtgcattta
      601 taaacgacat ctacttattt tggatttttc ggtttatgta ttgatgggtt tggtcgtgga
      661 taattgtaat ggagaaggtt tttaattgaa atggattaaa ggacgtcttt aaaagtgcaa
      721 cttatctaat cttttggcga aaggaacact taaagttggt gtcacggggc ataaatttcg
      781 agatttgcgt gaatgtgtaa atcagagacg tgttgggtta atctgtctta attggaaaga
      841 acggcttaat ctatctgtgc acggaccact tgggatgtac atacactttt agatctttta
      901 gtaaatgttt acagtccgat aaattataca tttgaacact ttaattcgaa gtgaatcagt
      961 ttttaaatag ttttataaga aataattttt ttaaatttaa tcttctgaga agttttactt
     1021 gcgtaaggct atttgtatat atcgccgtct ctcgccaaat tgctcatgta accaacgcaa
     1081 agattaacaa agagaccaaa ctttaaagta cacaatttat aatattgttt atctacacat
     1141 cctttcacaa caaatataac aggatcagaa aacttaagta aagtaaaact aactgaaatg
     1201 cacatttgtg taagtttgaa tcaaatgttt gatacaataa taatctccgc agattagttt
     1261 gaaaggtttc attcgaatga gtttgtgtca tttatgagtt ttgtgttttg gcatgagtga
     1321 attaggggca aattattgcc aaaccaggcg ttatgatccg ccctacagca gccaaagatg
     1381 aacccaatca aatctgaaca aataaagttt acagcggagg aaactcaaat aagcaagagt
     1441 tgcgagcaaa gttaaaatga atgagcataa agtttataaa agtcctcctg acagaaaaca
     1501 gccgctgaaa tcttttcctt aaaacaaaat aaacccagca gcgcaattac cgaaattgac
     1561 ggatacattt gttagctact aaaacaaacc aagtcttgtg cgatgctttc tttttcgtcc
     1621 caaataaatt ggacaagtca aaccattaac gctttctgta ggctatacat gtgattaaca
     1681 aatgatcaat gtgttaatgt gttttgtaag gggtctacaa gtagccattc ttggctaact
     1741 ctagcacatt ttacagtaat gtttattaat gtgcattgac cctgtaaaca aataaactaa
     1801 acatggttct gctcatgtga agtcacctgt atcttaatta acactgctgt tggatgctgt
     1861 gtttttgtat tcgggttaat attggatgtc tgtaactcaa gttcaagctg acaggtcgca
     1921 tctcgacagc ttctgtcgca tataaattat ttgatcgatt tttctgtaat tggacctagt
     1981 caaccacatc cactcgttta attcacgatt acttcacatt tcagaaacac actgccatga
     2041 ctctcttgat ttttttcttc ttctgctggt tataagtcgt cgtttgtgct ttcaccaaca
     2101 aacactgtgt aattcagctg cccacggcag cgccgctccg tgtttatcca gcagcgaggc
     2161 ggaatgaagc cggtattatt ccagcacttt ctctcccggg agcttgaaca aaccgccaaa
     2221 ccttcaacag tagcttgtta gcagaagtga agctaaaata acacacacac atatacacac
     2281 accgaggccc actgacgcca ccgtatgatt gcagcgctgc cctggaaaaa ggcgacttct
     2341 aaataaagtc aaagccttag tgtttgtagg tttttcctga acaggtttat cttgttcatg
     2401 aggtgttcgg gtgaacagca aaggggttgt gcggtcatac agccaaccat taaaacgaca
     2461 tattaatgct attttataca ttaggaagac atgctctgca gaagcaagct gtttaataaa
     2521 ataataacaa tgattattat aactaataaa ttacctgtgt gtgtattttt gtaggcttcc
     2581 atcagatcta tatccagagg aagacggcgg aaggattcct tcttacaaac tcgaattata
     2641 taaaaagaac tgttaaggtt tcactggatg atcttaaata ataaacaaaa gcacaattat
     2701 tttacaagaa aaaaacattt gtaaagtata aattaataca tttatattta aaataaaatt
     2761 taagtgagga aattaaacct accgaagtca cctgtggccg cagaactagt tctagaacta
     2821 tagtgagtcg tattacgtag atccagacaa gtttgtacaa aaaagcaggc tccaccatgg
     2881 ggacttccca tccggcgttc ctggtcttag gctgtcttct cacagggctg agcctaatcc
     2941 tctgccagct ttcattaccc tctatccttc caaatgaaaa tgaaaaggtt gtgcagctga
     3001 attcatcctt ttctctgaga tgctttgggg agagtgaagt gagctggcag taccccatgt
     3061 ctgaagaaga gagctccgat gtggaaatca gaaatgaaga aaacaacagc ggcctttttg
     3121 tgacggtctt ggaagtgagc agtgcctcgg cggcccacac agggttgtac acttgctatt
     3181 acaaccacac tcagacagaa gagaatgagc ttgaaggcag gcacatttac atctatgtgc
     3241 cagacccaga tgtagccttt gtacctctag gaatgacgga ttatttagtc atcgtggagg
     3301 atgatgattc tgccattata ccttgtcgca caactgatcc cgagactcct gtaaccttac
     3361 acaacagtga gggggtggta cctgcctcct acgacagcag acagggcttt aatgggacct
     3421 tcactgtagg gccctatatc tgtgaggcca ccgtcaaagg aaagaagttc cagaccatcc
     3481 catttaatgt ttatgcttta aaagcaacat cagagctgga tctagaaatg gaagctctta
     3541 aaaccgtgta taagtcaggg gaaacgattg tggtcacctg tgctgttttt aacaatgagg
     3601 tggttgacct tcaatggact taccctggag aagtgaaagg caaaggcatc acaatgctgg
     3661 aagaaatcaa agtcccatcc atcaaattgg tgtacacttt gacggtcccc gaggccacgg
     3721 tgaaagacag tggagattac gaatgtgctg cccgccaggc taccagggag gtcaaagaaa
     3781 tgaagaaagt cactatttct gtccatgaga aaggtttcat tgaaatcaaa cccaccttca
     3841 gccagttgga agctgtcaac ctgcatgaag tcaaacattt tgttgtagag gtgcgggcct
     3901 acccacctcc caggatatcc tggctgaaaa acaatctgac tctgattgaa aatctcactg
     3961 agatcaccac tgatgtggaa aagattcagg aaataagatg taataatgaa acttcctgga
     4021 ctattttggc caacaatgtc tcaaacatca tcacggagat ccactcccga gacaggagta
     4081 ccgtggaggg ccgtgtgact ttcgccaaag tggaggagac catcgccgtg cgatgcctgg
     4141 ctaagaatct ccttggagct gagaaccgag agctgaagct ggtggctccc accctgcgtt
     4201 ctgaactcac ggtggctgct gcagtcctgg tgctgttggt gattgtgatc atctcactta
     4261 ttgtcctggt tgtcatttgg aaacagaaac cgaggtatga aattcgctgg agggtcattg
     4321 aatcaatcag cccggatgga catgaatata tttatgtgga cccgatgcag ctgccttatg
     4381 actcaagatg ggagtttcca agagatggac tagtgcttgg tcgggtcttg gggtctggag
     4441 cgtttgggaa ggtggttgaa ggaacagcct atggattaag ccggtcccaa cctgtcatga
     4501 aagttgcagt gaagatgcta aaacccacgg ccagatccag tgaaaaacaa gctctcatgt
     4561 ctgaactgaa gataatgact cacctggggc cacatttgaa cattgtaaac ttgctgggag
     4621 cctgcaccaa gtcaggcccc atttacatca tcacagagta ttgcttctat ggagatttgg
     4681 tcaactattt gcataagaat agggatagct tcctgagcca ccacccagag aagccaaaga
     4741 aagagctgga tatctttgga ttgaaccctg ctgatgaaag cacacggagc tatgttattt
     4801 tatcttttga aaacaatggt gactacatgg acatgaagca ggctgatact acacagtatg
     4861 tccccatgct agaaaggaaa gaggtttcta aatattccga catccagaga tcactctatg
     4921 atcgtccagc ctcatataag aagaaatcta tgttagactc agaagtcaaa aacctccttt
     4981 cagatgataa ctcagaaggc cttactttat tggatttgtt gagcttcacc tatcaagttg
     5041 cccgaggaat ggagtttttg gcttcaaaaa attgtgtcca ccgtgatctg gctgctcgca
     5101 acgtcctcct ggcacaagga aaaattgtga agatctgtga ctttggcctg gccagagaca
     5161 tcatgcatga ttcgaactat gtgtcgaaag gcagtacctt tctgcccgtg aagtggatgg
     5221 ctcctgagag catctttgac aacctctaca ccacactgag tgatgtctgg tcttatggca
     5281 ttctgctctg ggagatcttt tcccttggtg gcacccctta ccccggcatg atggtggatt
     5341 ctactttcta caataagatc aagagtgggt accggatggc caagcctgac cacgctacca
     5401 gtgaagtcta cgagatcatg gtgaaatgct ggaacagtga gccggagaag agaccctcct
     5461 tttaccacct gagtgagatt gtggagaatc tgctgcctgg acaatataaa aagagttatg
     5521 aaaaaattca cctggacttc ctgaagagtg accatcctgc tgtggcacgc atgcgtgtgg
     5581 actcagacaa tgcatacatt ggtgtcacct acaaaaacga ggaagacaag ctgaaggact
     5641 gggagggtgg tctggatgag cagagactga gcgctgacag tggctacatc attcctctgc
     5701 ctgacattga ccctgtccct gaggaggagg acctgggcaa gaggaacaga cacagctcgc
     5761 agacctctga agagagtgcc attgagacgg gttccagcag ttccaccttc atcaagagag
     5821 aggacgagac cattgaagac atcgacatga tggacgacat cggcatagac tcttcagacc
     5881 tggtggaaga cagcttcctg tgatacccag ctttcttgta caaagtgggg gatccagaca
     5941 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     6001 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     6061 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     6121 ttttttccaa ctttattata catagttgat aattcactgg ccgtcgtttt acggtaccat
     6181 cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     6241 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     6301 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     6361 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     6421 ttatgatcct ctagatcaga tcgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     6481 cattaccctg ttatccctac gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     6541 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     6601 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     6661 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6721 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6781 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6841 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6901 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6961 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7021 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7081 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7141 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7201 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7261 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7321 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7381 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7441 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7501 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7561 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7621 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7681 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7741 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7801 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7861 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7921 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7981 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8041 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8101 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8161 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8221 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8281 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8341 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8401 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8461 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8521 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8581 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8641 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8701 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8761 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8821 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8881 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8941 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9001 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9061 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9121 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9181 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     9241 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gtagggataa
     9301 cagggtaatg cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg
     9361 tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata tcaagcttat
     9421 cgataccgtc gacctcgagc aggaaacagc tatgaccatg attacgccaa gctatcaact
     9481 ttgtatagaa aagttgtacg ccagtcgacg gtatcgataa gcttgatatc gaattcccca
     9541 cagcggacaa caggacaaaa cctctcactg ctacatgatc aatcctccac ccactgcccg
     9601 ctgcacttcc tgtctcacac acacacacac acacaagcag agccagtgcc atcaggtgtg
     9661 tgtgtgtgtg tgtcatggga catgcgtgtc ctcctattga ccttctgacc tcacaaacat
     9721 ctttctctga catcccagag accatctcca acggcaacca accacatcat cactgtcaca
     9781 tgctgcatga ctgcacacac acacacacac actttctcgc tcacacaaac atattgattc
     9841 acatttatac acacatttaa acacacaaac acatttaaat acacacacac acacacacac
     9901 acacatgatc ctataaactg cgcacaacac taagcaccag cagttttagt ttgctgaagc
     9961 atcagtttgt aacagaataa ctcagcctcc tcatgatctc ctcttcgctg ctcattcagg
    10021 caaaactgca gtttaaaagc gctgataaac aaaactccaa ctatggcaca cacactccct
    10081 tgatcagaga tgaattgatg aaattaatta tgtttttgtg tgcgtatttc tttattaaat
    10141 cacgcagacg cgcgcagttt tatctctgcg gtaatgcgga tcataataaa ctcgtgatta
    10201 aaaaactaat tacactgtcg cacgaacatg cgcgcattaa caacgaacac acgagtcatt
    10261 tcgataaata tacagctcag aaattacatt aaacatttat aagatatcaa aaacggtcaa
    10321 gagaaacgaa tttatttatg tttttataaa gcgcatttgc gagtcgctca aaaaactgta
    10381 tagtatgaac ataataatgt ttaaaaaaca cgaaatactg atacaaataa tactgttaat
    10441 aataaaaaca ttgttatttg tattaaatag cttgtggata tccagaaata atgcgtaatt
    10501 ctaaccgaca gatgatctaa aatcatttcg tcagtctctt atttgataga ctacaaatgc
    10561 ataaaaagta aaacgcgttt tcgtatttat taaattggtt ttagtttttt ttaattaaat
    10621 tataatatta taataaaata atcaattgta tttttattac tgacatgcac gcataataaa
    10681 cgcgctatac aaatgcgcat acacattacg cacctgcgcg tttgggttat ttccatttaa
    10741 ataaattcaa ctacaaaacc ccaaaacaaa aagtttaaaa acattttaat ttctgaattt
    10801 aaaaaataaa caaaatcaat catttaaaaa ttgtaggcag ggttgtatta acccaacgct
    10861 gggtcaaata tggactcaaa gtttttgcat ggcatttaaa tatttaatcc gtctgctgaa
    10921 tttgtccata tttgacaata atacatccca gctattttag agcgcatttc tgatctcttc
    10981 tttaccataa aaacgaaacc ccttgataaa gcagaatggc attaaactgg aaattaataa
    11041 taatgtaaac aattaactaa taacatatat aaaacaaaac gatttacaca aatagcgcag
    11101 gacacatttc cgcggtataa aagaagcctc cctgttctga caggcgatgc gacacacata
    11161 acaaatcact cttctcaagc agccacatta aataacagaa gaaacaatta acatgcacgc
    11221 gcatactaaa cagcacacat taacaaatgt ccctcactga caaacacccc ccagcttgat
    11281 gtttatgtcc tataaatgct cggagaccga gtgtcacttt ctccaaagcg gggtctcctc
    11341 cttcccaaag cctccctaag agcaacaggg caaaactcac atcaaagtgt gcagcaggcc
    11401 tcagatccgc gcgctttccc gtgtacatcc acagacccgc gagaaccgag acatgtgtgc
    11461 gtgtacgttt gaagggacac cgagaaaaag acgctctaaa gagagacaca cgtcttttct
    11521 cttttctttt ctttttttat atacacagaa ctgctttttg ttcctcattt tttcatttaa
    11581 gagggtaatg gaaggggggt gttcgtttga tcacatgatt acaattcacc tgagataaaa
    11641 caaaggacgc aaagaaagga gccgagcgcc ggagaccgag acagagagat gctctcatgg
    11701 tcaggatccc cttatcagag tcaacattca tggataatga aaaaggcaat gatttttggc
    11761 ttgggtgggg gggccgtcgt gagatggaaa tacaagaggt tattgttgac cagaacagcg
    11821 cgcgtgtttg tgtgcgtttg gcccgtcgtt tgcctctctt ctcgtctttc tatattttct
    11881 actaaagctg tttaaacgcg ttaaatgcgc tctctgtgtg tgtttatgcg cgctgatgca
    11941 attataacac taacacacat agcgcgacac gatgcaggag ttcattccag tgctgcgggc
    12001 ctgcgcgcgc agcatgaaca caccactgcg agcaaatctg caatattaat gaatatatat
    12061 aactaaagtg tgcactttct ggtttataac tgcagcaccc cgcatacacc acagtataaa
    12121 gtaatctaca atattaatga atataataaa caagaatgtt cacttttagg tttaagaatg
    12181 caggcctgcg cgcgcagcat cccgcgagca caacactaga aactaaactc caatattagt
    12241 taatatattc aattaaaatg tacactttct ggtttaaaac tgcaagtatg cgtgcgcagc
    12301 atccgacata cccaacacta gaaactaatc tacaatattt atgaaagtat tcaactaaaa
    12361 tgttcacttt ctggtttaaa actgcaagta tacgcgtgcg cagcatccga catacccaac
    12421 actagaaatt aatctaaatt attaattaaa gtattcaact gaattgttca ctttctggtt
    12481 taaaactgca agtatgggtg cgcagcataa aacataccca acactagaaa ctaatctaca
    12541 atattaatgg atgtattcaa ctaaaatgtt cactttctgg tttaaaactg caggaatgcg
    12601 cgcgcaacac cctgcggata actttaaaaa caattccgca atcagctaaa actgaaaagt
    12661 tcagttgatt aacgaatgta ggcatgcgca aaatattata aatgcgcaca cacatctatt
    12721 taatacatta aaactaaggc taaatgttca ctttcaccat tttggggaaa atgaaaattc
    12781 agtgtccccc ttaagtggcc agctttaggg caataggcga ccctccataa gtagcataaa
    12841 atcgtaggct attctgggca ccctttggca tcgccacgcg ttaaacaggg aatggagagg
    12901 ttattgcgca cagcaggtgc gcagagtcgc gtccgctaat ggcgctggag ctgctgacag
    12961 gtccgctggc gctctgcggg gtggaagcgc tcgaaacccc gcacaacccc tgattcaccc
    13021 tgaggcacag ccaggccaac aacgggccag tgtgatcgga aaagagttga aagaatctcc
    13081 gtgacaccaa tgaggagcca aagaccacct gtgaaataca tcacatgtca gacaaaagca
    13141 tattaagctg atcaaatatt gcgtgcataa ccgcattaca tcacaaagaa aaacattagc
    13201 tagctatggg taacttaata agtgttctac atttgcactg cagccatggt tgtgagccga
    13261 aaaggtttgc gcaataatat tcaccccccg agatttacat tttttttttg cggttctctg
    13321 atgtcgatta atggccacgt ggccttcagg taaacacatt taatatttaa cttatgtgtt
    13381 ttaattagtg tgttccgaat tattcggtta tttttattta acatttttat taattagctt
    13441 aattctgatc cactcccaac tcttattaac gaatcacaaa tcaaatggat agttttaatt
    13501 gcacaattag cctgtcaaaa cttaacttaa agctgttaat gcactgcttt taagtttgaa
    13561 aaataaagaa aaattattag aaaacttaag attagatttt aacatatgtt gaaattatca
    13621 aatcggcatg tttatgataa aacaacttta cctaaaacta aaattcagat caacaaaaac
    13681 ctaaatgaag ttacatatat atatatatgt gtgtgtgtgt gtgtatatat atata
//
