LOCUS       sequence-183617-        9985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector forSRF with Dam-V5 using Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label:  pLgw EcoDam-V5-SRF
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1329..1353)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             1884..2064
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2090..2107)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2096..2320
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2366..2794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2453..2472)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2663..2684
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2803..2823)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2808..3137
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2988..3123
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3050..3069
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3185..3232
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3251..3625
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3755..3876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3792..3811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3846..3865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3925..3941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3925..3941)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3938..3960)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3949..3965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3973..3979,3980..3997,3998..4003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4018..4039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4156..4173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4327..4915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4407..4426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5086..5877,5878..5946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5709..5728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5947..6051)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6126..6145)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6317..6696
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6698..6896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6851..6871
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6914..7094
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7141..7266
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7759..7992
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8177..8221
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8683..8921
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     8868..8889
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             9816..9857
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    9873..9897
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caccgccggg gccctctaca gcggcagcga gggcgactcc gagtccggcg aggaggagga
       61 gctgggcgcc gagcggcgcg gcctcaagcg gagcctgagc gagatggagc tcggcgtggt
      121 ggtcggtggg cctgaggcgg cggcggccgc cgggggctac gggccggtga gcggcgcggt
      181 gagcggggcc aagccgggga agaagacccg gggccgcgtg aagatcaaga tggagttcat
      241 cgacaacaag ctgcggcgct acacgacctt cagcaagagg aagacgggca tcatgaagaa
      301 ggcctatgag ctgtccacgc tgacagggac acaggtgctg ttgctggtgg ccagtgagac
      361 aggccatgtg tatacctttg ccacccgcaa actgcagccc atgatcacca gtgagaccgg
      421 caaggcgctg attcagacct gcctcaactc gccagactct ccgccccgct cagaccccac
      481 cacagaccag agaatgagtg ccactggctt tgaagagcca gatctcacct accaggtgtc
      541 ggaatctgac agcagtgggg aaaccaagga cacactgaag ccagcattca cagtcaccaa
      601 cctgccgggt accacctcca caatccagac agcacccagc acctctacca ccatgcaagt
      661 cagcagcggc ccctccttcc ccatcaccaa ctacctggca ccagtgtctg ctagtgtcag
      721 ccccagcgct gtcagcagtg ccaacgggac tgtgctcaag agtacaggca gcggccctgt
      781 ctcctctggg ggccttatgc agctgcctac cagcttcacc ctcatgcctg gtggggcagt
      841 ggcccagcag gtccctgtgc aggccattca tgtgcaccag gccccacagc aagcgtctcc
      901 ctctcgtgac agcagcacag acctcacgca gacctcctcc agcgggacag tgacgttgcc
      961 cgccaccatc atgacgtcgt ctgtacccac aactgtgggt ggccacatga tgtaccctag
     1021 tccccatgca gtgatgtatg cccccacctc aggcctggct gatggcagcc tcaccgtgct
     1081 caatgccttc tctcaggcac catccaccat gcaggtgtcc cacagccagg tccaggagcc
     1141 aggtggtgtc cctcaggtgt tcctgacagc accgtctggg accgtgcaga tccctgtctc
     1201 tgcagttcag cttcaccaga tggctgtgat agggcagcaa gctgggagca gcagcaacct
     1261 caccgagcta caggtggtga acctggatgc cacccacagc accaagagtg aatgaaaggg
     1321 cgaattcgac ccagctttct tgtacaaagt ggttcgatcg aaccgcggtc tagactcgag
     1381 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     1441 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     1501 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     1561 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     1621 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     1681 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     1741 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     1801 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     1861 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     1921 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     1981 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2041 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     2101 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     2161 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     2221 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     2281 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     2341 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2401 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2461 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2521 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2581 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2641 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2701 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2761 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     2821 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2881 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2941 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3001 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3061 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3121 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     3181 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     3241 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     3301 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     3361 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     3421 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     3481 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     3541 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     3601 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     3661 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     3721 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     3781 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3841 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     3901 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3961 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4021 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4081 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4141 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4201 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4261 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4321 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4381 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4441 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4501 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4561 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4621 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4681 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4741 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4801 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4861 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5041 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5101 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5161 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5221 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5281 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5341 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5401 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5461 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5521 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5581 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5641 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5701 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5761 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5821 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5881 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5941 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6001 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6061 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     6121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     6181 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     6241 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     6301 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     6361 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     6421 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     6481 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     6541 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     6601 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     6661 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     6721 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     6781 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     6841 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     6901 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6961 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7021 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7081 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7141 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7201 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7261 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7321 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7381 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7441 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7501 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7561 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7621 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7681 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7741 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7801 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7861 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7921 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7981 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8041 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8101 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8161 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8221 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8281 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8341 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     8401 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     8461 gaacggatcc cccgggctgc aggaattcag atctcggccg catattaagt gcattgttct
     8521 cgataccgct aagtgcattg ttctcgttag ctcgatggac aagtgcattg ttctcttgct
     8581 gaaagctcga tggacaagtg cattgttctc ttgctgaaag ctcgatggac aagtgcattg
     8641 ttctcttgct gaaagctcag tacccgggag taccctcgac cgccggagta taaatagagg
     8701 cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg
     8761 aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa
     8821 gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa
     8881 tctgccaaga agtaattatt gaatacaaga agagaactct gaatactttc aacaagttac
     8941 cgagaaagaa gaactcacac acagctagcg ccaccatgaa gaaaaatcgc gcttttttga
     9001 agtgggcagg gggcaagtat cccctgcttg atgatattaa acggcatttg cccaagggcg
     9061 aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc gacttttctc
     9121 gttatatcct tgccgatatc aatagcgacc tgatcagtct ctataacatt gtgaagatgc
     9181 gtactgatga gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca aattgcgccg
     9241 aggtttacta tcagttccgc gaagagttca acaaaagcca ggatccgttc cgtcgggcgg
     9301 tactgttttt atatttgaac cgctacggtt acaacggcct gtgtcgttac aatctgcgcg
     9361 gtgagtttaa cgtgccgttc ggccgctaca aaaaacccta tttcccggaa gcagagttgt
     9421 atcacttcgc tgaaaaagcg cagaatgcct ttttctattg tgagtcttac gccgatagca
     9481 tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc gccttatgca ccgctgtctg
     9541 cgaccgccaa ctttacggcg tatcacacaa acagttttac gcttgaacaa caagcgcatc
     9601 tggcggagat cgccgaaggt ctggttgagc gccatattcc agtgctgatc tccaatcacg
     9661 atacgatgtt aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc aaagttcgac
     9721 gcagtataag cagcaacggc ggcacacgta aaaaggtgga cgaactgctg gctttgtaca
     9781 aaccaggagt cgtttcaccc gcgaaaaaaa ccggtggtaa gcctatccct aaccctctcc
     9841 tcggtctcga ttctacgatc gatttcgatc aaacaagttt gtacaaaaaa gcaggctccg
     9901 aattcgccct tatgttaccg agccaagctg gggccgcggc ggctctgggc cggggctcgg
     9961 ccctgggggg caacctgaac cggac
//
