LOCUS       sequence-183618-       10111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector forSRF with Dam-V5 using Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de 
            Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA,
            Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, 
            Harvey RP
  TITLE     NKX2-5 mutations causative for congenital heart disease retain 
            functionality and are directed to hundreds of targets.
  JOURNAL   Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942.
  PUBMED    26146939
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-07-6"; volume: "4. doi"; pages: " 10.7554/eLife.06942"
COMMENT     Sequence Label:  pLgw EcoDam-V5-SRF
FEATURES             Location/Qualifiers
     source          1..10111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2367..2605
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2552..2573
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3500..3541
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3557..3581
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5124..5148)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             5679..5859
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5885..5902)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5891..6115
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6161..6589
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6248..6267)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6458..6479
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6598..6618)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6603..6932
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6783..6918
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6845..6864
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6980..7027
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7046..7420
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7550..7671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7587..7606)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7641..7660
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7720..7736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7720..7736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7733..7755)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7744..7760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7768..7774,7775..7792,7793..7798))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7813..7834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7951..7968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8122..8710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8202..8221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8881..9672,9673..9741))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9504..9523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9742..9846)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9921..9940)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcccccggg
     2161 ctgcaggaat tcagatctcg gccgcatatt aagtgcattg ttctcgatac cgctaagtgc
     2221 attgttctcg ttagctcgat ggacaagtgc attgttctct tgctgaaagc tcgatggaca
     2281 agtgcattgt tctcttgctg aaagctcgat ggacaagtgc attgttctct tgctgaaagc
     2341 tcagtacccg ggagtaccct cgaccgccgg agtataaata gaggcgcttc gtctacggag
     2401 cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa
     2461 acaagcgcag ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat
     2521 taaaagtaac cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc aagaagtaat
     2581 tattgaatac aagaagagaa ctctgaatac tttcaacaag ttaccgagaa agaagaactc
     2641 acacacagct agcgccacca tgaagaaaaa tcgcgctttt ttgaagtggg cagggggcaa
     2701 gtatcccctg cttgatgata ttaaacggca tttgcccaag ggcgaatgtc tggttgagcc
     2761 ttttgtaggt gccgggtcgg tgtttctcaa caccgacttt tctcgttata tccttgccga
     2821 tatcaatagc gacctgatca gtctctataa cattgtgaag atgcgtactg atgagtacgt
     2881 acaggccgca cgcgagctgt ttgttcccga aacaaattgc gccgaggttt actatcagtt
     2941 ccgcgaagag ttcaacaaaa gccaggatcc gttccgtcgg gcggtactgt ttttatattt
     3001 gaaccgctac ggttacaacg gcctgtgtcg ttacaatctg cgcggtgagt ttaacgtgcc
     3061 gttcggccgc tacaaaaaac cctatttccc ggaagcagag ttgtatcact tcgctgaaaa
     3121 agcgcagaat gcctttttct attgtgagtc ttacgccgat agcatggcgc gcgcagatga
     3181 tgcatccgtc gtctattgcg atccgcctta tgcaccgctg tctgcgaccg ccaactttac
     3241 ggcgtatcac acaaacagtt ttacgcttga acaacaagcg catctggcgg agatcgccga
     3301 aggtctggtt gagcgccata ttccagtgct gatctccaat cacgatacga tgttaacgcg
     3361 tgagtggtat cagcgcgcaa aattgcatgt cgtcaaagtt cgacgcagta taagcagcaa
     3421 cggcggcaca cgtaaaaagg tggacgaact gctggctttg tacaaaccag gagtcgtttc
     3481 acccgcgaaa aaaaccggtg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     3541 gatcgatttc gatcaaacaa gtttgtacaa aaaagcaggc tccgaattcg cccttatgtt
     3601 accgagccaa gctggggccg cggcggctct gggccggggc tcggccctgg ggggcaacct
     3661 gaaccggacc ccgacggggc ggccgggcgg cggcggcggg actcgcgggg cgaacggggg
     3721 ccgggtcccc gggaacggcg cggggctcgg ccagagtcgt ctggagcggg aggctgcagc
     3781 ggcagcggcg cccaccgccg gggccctcta cagcggcagc gagggcgact ccgagtccgg
     3841 cgaggaggag gagctgggcg ccgagcggcg cggcctcaag cggagcctga gcgagatgga
     3901 gctcggcgtg gtggtcggtg ggcctgaggc ggcggcggcg gccgccgggg gctacgggcc
     3961 ggtgagcggc gcggtgagcg gggccaagcc ggggaagaag acccggggcc gcgtgaagat
     4021 caagatggag ttcatcgaca acaagctgcg gcgctacacg accttcagca agaggaagac
     4081 gggcatcatg aagaaggcct atgagctgtc cacgctgaca gggacacagg tgctgttgct
     4141 ggtggccagt gagacaggcc atgtgtatac ctttgccacc cgcaaactgc agcccatgat
     4201 caccagtgag accggcaagg cgctgattca gacctgcctc aactcgccag actctccgcc
     4261 ccgctcagac cccaccacag accagagaat gagtgccact ggctttgaag agccagatct
     4321 cacctaccag gtgtcggaat ctgacagcag tggggaaacc aaggacacac tgaagccagc
     4381 attcacagtc accaacctgc cgggtaccac ctccacaatc cagacagcac ccagcacctc
     4441 taccaccatg caagtcagca gcggcccctc cttccccatc accaactacc tggcaccagt
     4501 gtctgctagt gtcagcccca gcgctgtcag cagtgccaac gggactgtgc tcaagagtac
     4561 aggcagcggc cctgtctcct ctgggggcct tatgcagctg cctaccagct tcaccctcat
     4621 gcctggtggg gcagtggccc agcaggtccc tgtgcaggcc attcatgtgc accaggcccc
     4681 acagcaagcg tctccctctc gtgacagcag cacagacctc acgcagacct cctccagcgg
     4741 gacagtgacg ttgcccgcca ccatcatgac gtcgtctgta cccacaactg tgggtggcca
     4801 catgatgtac cctagtcccc atgcagtgat gtatgccccc acctcaggcc tggctgatgg
     4861 cagcctcacc gtgctcaatg ccttctctca ggcaccatcc accatgcagg tgtcccacag
     4921 ccaggtccag gagccaggtg gtgtccctca ggtgttcctg acagcaccgt ctgggaccgt
     4981 gcagatccct gtctctgcag ttcagcttca ccagatggct gtgatagggc agcaagctgg
     5041 gagcagcagc aacctcaccg agctacaggt ggtgaacctg gatgccaccc acagcaccaa
     5101 gagtgaatga aagggcgaat tcgacccagc tttcttgtac aaagtggttc gatcgaaccg
     5161 cggtctagac tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     5221 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     5281 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5341 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     5401 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     5461 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     5521 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     5581 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     5641 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     5701 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5761 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5821 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     5881 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5941 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6001 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6061 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6121 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6181 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6241 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6301 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6361 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6421 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6481 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6541 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     6601 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6661 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6721 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6781 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6841 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6901 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     6961 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7021 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     7081 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7141 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     7201 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     7261 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     7321 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     7381 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     7441 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7501 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7561 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7621 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7681 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7741 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7801 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7861 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7921 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8521 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9481 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9841 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     9901 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     9961 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    10021 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    10081 cttcgcgatg tacgggccag atatacgcgt t
//
