LOCUS       sequence-183640-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_104 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_104
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    570..704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(594..613)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     648..667
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(815..831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(815..831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(828..850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    839..855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(863..869,870..887,888..893))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    908..929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gaagaacaag caaacaagat tctcgtagaa
      121 ctactgcaaa aagccagtaa tggaatagac gcggctgttt catttagcca ggcacagatt
      181 cctgatgttg ttcatcagtt gctgctatgg aatatggttg acagtctgat taaaacatta
      241 atagccattc taacaatccc actggttttc tggtttatga agaagcagtg ccaaagagtt
      301 gagacaggta aaatcggtga tgaaggatac tcatgggaga ggggaaatcc caaatacagg
      361 ccgacaatgg tttgggatag caaaggagat attaaccttc ttatcatgcc attggttgga
      421 gttttgactc tgtgggggtt ttttattatt ggtgtagtaa ccaatatgac ttggttaaaa
      481 atttggctgg ccccaaaact ttaccttatc gaatatgcag catcattggt taagtgaatc
      541 ggtaccctcg agaagcttgc ggccgcgggg atccagacat gataagatac attgatgagt
      601 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      661 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      721 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcggat cctctagagt
      781 cgatctgcag gcatgctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      841 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      901 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      961 cggnaaanct gtcgtgncag ctgcattnat gaatcggnca acgcg
//
