LOCUS       sequence-183648-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_102 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_102
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    527..661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(551..570)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     605..624
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(772..788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(772..788)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(785..807)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    796..812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(820..826,827..844,845..850))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    865..886
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggagggggcg gttcggtggc ggaggttccc gaattcgtcg acggagatct gatatggatt
       61 cagatttaga gccagagatg attgctgaca caatagaagg gatggaagga gaatttaccg
      121 ataaaataga gcaacttctc gccattatta aaaatgaatc tggttatgct gaacgcctca
      181 aggaagaggc aaagtcacta aatgagcgag ccgcagtaat tcaaaataag attgacagca
      241 ttatggcgta tatagcgtca tcgcttgaaa tggttggcaa gaaaaagatt cgagcaggta
      301 ttcaccaggt aacaatccgc aaaccgtcag aaactgtaga aatcatcgac tcaagcgccc
      361 ttcctcctga atacgttgag tttgaaacga caattaaagc cgacaaactg gcaatcaaac
      421 accaactaaa agcaggaata aatatccccg gcgctcaact caaagttggg aaaccttcac
      481 ttcttatcaa atgaatcggt accctcgaga agcttgcggc cgcggggatc cagacatgat
      541 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
      601 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      661 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
      721 ttcggatcct ctagagtcga tctgcaggca tgctagcttg gcgtaatcat ggtcatagct
      781 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      841 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      901 actgccgctt tccagtcggg aaacc
//
