LOCUS       sequence-183649-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_246 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_246
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(927..946)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg agaatactcg ttgctggcgc aacaggaagt
      121 attggtattc atgttgtgaa tactgctatt gcaatgggcc atcagcccgt gactctggtc
      181 aggaatcggc ggaaaatcaa gttactccct cgtggaacag atattttcta tggcgatgtt
      241 tcaatacctg aaacacttac cgatttaccg aaagatattg atgccatcat ttttacactc
      301 ggctctgatg gtcagggacg tattggcgcc agagcaatag attacggcgg tgtacgtaac
      361 attttgcgaa tattcatgga tacacctgtt cgcatcgccc tgatgacaac gattggcgta
      421 actgagcggc tcagtagctg gaatcaacgt actgaagttc atgactggaa gagacgctct
      481 gaacgcctgg tcagagccag tgggcatatc tacactatcg tcagacccgg ctggtttgat
      541 tacaacaatg atgatgagca cagaatcgtc atgcttcagg gggacagacg ccatgcggga
      601 acgccggaag atggtgttat ctcccgggag caaatcgccc aggttcttgt aacagctctg
      661 agcaacgatg cggcaaaaaa taaaacgttc gaactggtcg cagagcgagg agaagcacag
      721 caggatctca ctcctctgtt tgcagagctg cggaatgata atccacaaaa aaatgatggg
      781 gttttcgata tagacaatat gccccttact gaagaacctg agtgcgttat taacgacctg
      841 aatctgtatt cgaaaaattc aaaaatctga atcggtaccc tcgagaagct tgcggccgcg
      901 gggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
      961 gaaaaaaatg ctttatttgt gaaatttgtg atgctanngc tttatttnta nccattanna
     1021 gctgcnataa a
//
