LOCUS       sequence-183655-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_159 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_159
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             575..586
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttggaggggg gcggttccgg tggcggaggt tcccgaattc gtcgacggag atctgatatg
       61 aaaattaaca agatattatc atctgcaaca ctattgtatg gtatgtcaat ggccatgtcg
      121 gtcgggagtt gtgcaacacc tgtccagact aatcttcctg gttacacccc gggtgcagat
      181 atcattagtg tttcaccgac cagaaaccag gtcgatctca ttggtgatgt tgtttattcc
      241 cagataaaag gaactcgttc tgtcagacag cttcacatgt cagttcttgt cccgcgaaca
      301 aatgatttaa aaccagccat tatttattat cccggcggcg gattcatgtc ttctgaacat
      361 gacaaattta ttgaaatgag aatggctctg gcagaagctg gttttgttgt ggccgctgta
      421 gaatacagaa caattcctga tacatttcca gcaccagttg aggatggaaa agctgcaata
      481 cgttacctga gagagcatgc cagcaattat gggattgatc ctcaaagaat cggagttctg
      541 ggtgactctg ccggtggatg gcttgcccag atgatgggaa ctacaaatgg tgacaaaacc
      601 tttgataaag gtgactttct tcagcaatcc tcagatgttc aggcagttgc cacactttat
      661 gggatttctg acttgttgaa tattggcgag gggttccctg aatcagtgca ggaggttcat
      721 cgctctcctg ccgtaaccga agccttaatg atcaatggcc ctgcattcag aaattttgcg
      781 ggagccccca tcacagcgtc aaaagaaaaa gcgctaaacg ccagtccaat cggacatatg
      841 aaaggagtaa aacccccatt tcttattatg catggtagca agacactctg gtttcacctg
      901 agcaaagcgc caaactattc agatgt
//
