LOCUS       sequence-183657-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_583 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_583
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    414..548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(438..457)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     492..511
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(659..675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(659..675)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(672..694)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    683..699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(707..713,714..731,732..737))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    753..774
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(891..908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg agtgatgaaa tttacctgac cataaccgga
      121 gaacaacaag gatgtatttc ctctcgttgc ggaacgagcg catcaatagg taatcgctgg
      181 cagattggcc atgaagatga aatcttcgcg ttctcgctat ctaatagtat tacgaataca
      241 ggcaaagggt cacaattaca tggccttagt ttctgtaagt tgattgacaa aagctccccg
      301 ttacttatta acgctattaa caataacgag cagttgttca tggagtttga cttctatcgg
      361 atcaatagat ttggtcgatg aatcggtacc ctcgagaagc ttgcggccgc ggggatccag
      421 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      481 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      541 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      601 aggttttttc ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt
      661 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      721 gaagcataaa gtgtaaagcc tgggggtgcc taatgagtga gctaactcac attaattgcg
      781 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      841 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      901 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aanggcggta
      961 ntacgntnnt ccacagaatc nnggatnacg cnnnaagaac atgtgagcaa aa
//
