LOCUS       sequence-183663-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_314 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_314
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    556..690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(580..599)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     634..653
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tggagggggc ggttccggtg gcggaggttc ccgaattcgt cgacggagat ctgatatggc
       61 tacgatcccc actcagacac acccgttact tgctgttccc ttcaatgctg ccacagattt
      121 taccgtactg gctgaccact gtgaaaactt cgccgaaacc ctgatcgaaa gcgacgatcc
      181 gatgctgcgg ctggcgcttt gcgggcggct tgccgcctgt ctgactctgc tgcaacccac
      241 gctgaacgac cctatcccgt cccatctgac tgacagcctg accgttgatg ctctacctgc
      301 cgatatgccc cgatttgtcc ccgatgctga attactttgt gattactcac tgacgctggc
      361 gcaacttctt atcgggcagg ggatgcagcc aaaagtagag cagacgataa caggcttatt
      421 atgtgaactg gtctggtatt ttgccgccga aatgaaagcc ccgcgttggc tgcgtactgc
      481 cggtggcgtg aagtttatcg gcggtgtgga ggccaaagca tgaatcggta ccctcgagaa
      541 gcttgcggcc gcggggatcc agacatgata agatacattg atgagtttgg acaaaccaca
      601 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
      661 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
      721 caggttcagg ggagtgtggg g
//
