LOCUS       sequence-183664-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_321 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_321
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    730..864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(754..773)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     808..827
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gaggggcggt tccggtggcg gaggttcccg aattcgtcga cggagatctg atatggcaaa
       61 aatattacct tctatatttg gcgcggatat tttacgcctg aaagatgaaa ttcattttct
      121 ggaagaggaa caaactgaaa tattgcacgt tgatttaatg gatggcactt acgtcagtaa
      181 tattgccttt ggcccaaatc aaattgcagc aatgaaaaaa gccagttcga tgatctttga
      241 tgtgcatatg atgctggcaa atcctgagcg acatattgat gatgtaatca ataccggggc
      301 ggagatgatt tctgtacatt atgaatctac gccacatgta cattatatta ttcaaaaaat
      361 caaaaaagcg gggcgcaaag caggtgttgt gcttaatcct gggacacctg aatctgtcat
      421 cgagtattta ttagatgata ttgattatat attgttaatg acaattaacc ccgggcaacc
      481 aggccagaca tttattcata aaagtctgga gaaaatccgt aataccaaaa aaatgatatc
      541 tggcagagat attcaaattg aagtggatgg tggcgttaat cttgataatg tgaaacaagt
      601 taaagaagcg ggggctgacc ttattgttgt tggcggtgca ttatttaaca aacagcctca
      661 tgaaagttat caggcattaa aaatcgctgt tcagtgaatc ggtaccctcg agaagcttgc
      721 ggccgcgggg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
      781 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      841 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      901 caggggaggt gtgggaggtt ttttcggatc ctctagagtc gatctgcagg catgctagct
      961 tggccgt
//
