LOCUS       sequence-183669-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_355 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_355
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    639..775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(663..682)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     719..738
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gggcggttcg gtggcggagg ttcccgaatt cgtcgacgga gatctgatat gagaaataga
       61 atacttacct tgatgcaagc cagtgcgatt tttatgtttc ttacaaaatt gccgatgact
      121 actgtacttg tggttatatt atccttagca acaatttttt ataaacttct tcagaataaa
      181 ctatactttg ataagttaaa taataaaaca atagtggtaa ttatcgcaat gttgctatat
      241 atgttatgtc gaattatgtc gcaatacgtg gtaggtatag atttatatga tgtggacatt
      301 gattatatag tgacaatatc attagcgttt atattgtttg tatctatttt atttcatcga
      361 gatgcatatg caatattgta tgcaattata gcatcttcat tatttactgg aggatgggca
      421 atatttgaat taatatttaa ttttaatact ctatcttcac ggtttagcga tcctgggaat
      481 atttttacat gtcaggtaat agagtgccaa cagcattcta ttataatgaa aatgatatgc
      541 tttatttttt ggttttgttc ttaccaatta cctttttttt ctttaggaga cgcttggtgt
      601 cattgaatcg gtaccctcga gaagcttgcg gccgcgggga tccagacatg ataagataca
      661 ttgatgagtt tggacaaacc acaactagaa tgcagttgaa aaaaatgctt ttatttgtga
      721 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
      781 caacaattgc attcatttta tgtttcaggt tcaggggagg tgtgggaggt tttttcggat
      841 cctctagagt cgatctgcag gcatgctagc ttggcgtaat catggt
//
